Abnormal development and transport and increased sister-chromatid exchange in preimplantation embryos following superovulation in mice

Elbling, Leonilla; Colot, M.

doi:10.1016/0165-1161(85)90057-3

Cited by 58 publications

(31 citation statements)

References 27 publications

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“…Treated males were then mated with untreated females and embryos were collected 0.5-3.5 days later by flushing the oviduct or uterus. All matings were natural because superovulation has been reported to increase the rate of sister chromatid exchange (Elbling and Colot 1985a). Exactly as predicted from the normal sperm cycle (Monesi 1962), the label could be detected with the anti-BrdU antibody in some of the embryos derived from mating with males that had been exposed to BrdU for 35 days.…”

Section: Mouse Sperm Dna Can Be Labeled With Brdumentioning

confidence: 84%

Paternal DNA strands segregate to both trophectoderm and inner cell mass of the developing mouse embryo.

Ito¹,

McGhee²,

Schultz³

1988

Genes Dev.

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The localization of sperm DNA strands was examined in preimplantation mouse embryos. Male mice were treated with bromodeoxyuridine (BrdU) to label germ-cell-line DNA and were then mated with unlabeled females. Sperm DNA strands in early embryos derived from these matings could be detected by means of a fluorescent antibody specific to BrdU. The position and number of fluorescent spots detected in the developing embryos are consistent with a model in which paternal DNA strands segregate at random into both the trophectoderm and the inner cell mass. Although we could not follow the segregation of individual paternal chromosomes, we could detect no overall segregation pattern of the sperm DNA strands that could be obviously related to chromosome imprinting.

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Section: Mouse Sperm Dna Can Be Labeled With Brdumentioning

confidence: 84%

Paternal DNA strands segregate to both trophectoderm and inner cell mass of the developing mouse embryo.

Ito¹,

McGhee²,

Schultz³

1988

Genes Dev.

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“…However, more recent studies indicate that exogenous gonadotrophin treatment contributes to increased frequency of chromosomal abnormalities. Mouse embryos originating from stimulated females showed a fourfold increase in sister chromatid exchange frequency than embryos from spontaneous ovulations, which is suggestive of induced-DNA lesion by ovarian stimulation (Elbling & Colot 1985). Moreover, when compared to zygotes derived from spontaneous ovulation, mouse zygotes obtained after ovarian stimulation showed an increased rate of chromosomal aberrations in the female pronucleus and compromised embryo development (Vogel & Spielmann 1992).…”

Section: Does Ovarian Stimulation Disrupt Chromosomal Competence Of Tmentioning

confidence: 93%

The impact of ovarian stimulation for IVF on the developing embryo

Santos¹,

Kuijk²,

Macklon³

2010

Reproduction

174

128

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The use of assisted reproductive technologies (ART) has been increasing over the past three decades, and, in developed countries, ART account for 1-3% of annual births. In an attempt to compensate for inefficiencies in IVF procedures, patients undergo ovarian stimulation using high doses of exogenous gonadotrophins to allow retrieval of multiple oocytes in a single cycle. Although ovarian stimulation has an important role in ART, it may also have detrimental effects on oogenesis, embryo quality, endometrial receptivity and perinatal outcomes. In this review, we consider the evidence for these effects and address possible underlying mechanisms. We conclude that such mechanisms are still poorly understood, and further knowledge is needed in order to increase the safety of ovarian stimulation and to reduce potential effects on embryo development and implantation, which will ultimately be translated into increased pregnancy rates and healthy offspring.

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“…The relationship between the elevated FSH dose used for ovarian stimulation and the embryo quality remains a topic of heated discussion among practitioners of assisted reproduction. There is growing evidence in animal studies to support the hypothesis that elevated FSH is an underlying cause of embryo defects [9,10,39]. However, whereas animal studies seem to support an association between FSH exposure and embryonic defects, human studies have been more inconsistent [25,26,31,37,45].…”

Section: Discussionmentioning

confidence: 99%

Patient selection criteria for blastocyst transfers in extended embryo culture programs

Braga

Setti

Figueira

et al. 2012

J Assist Reprod Genet

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Purpose To identify the correlation between different cycles, patient factors and blastocyst characteristics. Methods The study included 420 patients undergoing ICSI cycles and 2781 graded blastocysts, which took into account the blastocyst quality. The correlations between the blastocyst parameters and the patient and cycle characteristics were assessed. Results The blastocyst development was negatively correlated with the maternal age, BMI and dose of FSH. The ICM was negatively correlated with the FSH dose, whereas the TE quality was influenced by the FSH dose, the maternal age and the number of retrieved oocytes. The embryo morphology on days two and three may predict the blastocyst developmental competence. Conclusions Older patients and patients with high BMI should not be included in extended embryo culture programmes. The extended culture may not favour embryos with poor morphology on days two and three of development. Additionally, a lower ovarian stimulation and decreased oocyte yields may lead to the development of high-quality blastocysts.

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Abnormal development and transport and increased sister-chromatid exchange in preimplantation embryos following superovulation in mice

Cited by 58 publications

References 27 publications

Paternal DNA strands segregate to both trophectoderm and inner cell mass of the developing mouse embryo.

Paternal DNA strands segregate to both trophectoderm and inner cell mass of the developing mouse embryo.

The impact of ovarian stimulation for IVF on the developing embryo

Patient selection criteria for blastocyst transfers in extended embryo culture programs

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