Abiotic Environmental Factors Affecting the Formation of Microbial Biofilms

Пономарева, А. Л.; Бузолева, Л. С.; Богатыренко, Е. А.

doi:10.1134/s106235901805014x

Cited by 23 publications

(16 citation statements)

References 41 publications

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“…We note that the reference strains of C. pseudotuberculosis were able to form biofilm on an abiotic surface. In general, the bacterial characteristics that determine the degree of attachment to such surfaces are of a physical–chemical nature ( 49 ), such as temperature, pH, and ionic strength, and most notably hydrophobicity, which is determined by the general composition of the bacterial surface ( 50 ). In C. pseudotuberculosis , N-acetylmuramoyl-L-alanine amidase and galactose-1-phosphate uridylyltransferase are involved in biofilm formation and exopolysaccharide biosynthesis ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

“…When an S. epidermidis biofilm was treated with ethanolic and ethyl acetate extracts of propolis, the decrease in survival was only 10%. On the other hand, the action of chitosan-propolis nanoparticles reduced survival by 70%, resulting in a breakdown of the biofilm and a decrease in the number of bacteria ( 49 ). Bryan et al ( 70 ) observed that the biofilm of S. aureus was more susceptible to the ethanolic extract of Russian propolis (3% w/v) when compared to the E. coli biofilm, and only the treatment with propolis at 20% w/v resulted in a complete inactivation of the bacterial biofilms, indicating that the antibiofilm activity is dose-dependent.…”

Section: Discussionmentioning

confidence: 99%

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Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

et al. 2021

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Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis in small ruminants, a chronic disease characterized by the development of granulomas in superficial and visceral lymph nodes as well as in several organs. An important characteristic of the infection with this bacterium is the formation of a biofilm and the absence of effective antibiotic therapy against the disease. From this scenario, the objective of this study was to evaluate the susceptibility of C. pseudotuberculosis to conventional antibiotics and to red, green, and brown propolis extracts obtained by the supercritical and ethanolic extraction methods as well as its activity in the bacterial biofilm. The results of the sensitivity test using antibiotics indicated a sensitivity of C. pseudotuberculosis strains to the antimicrobial agents. The ethanolic extract of green propolis and the supercritical red propolis extract showed the best antibacterial activities against planktonic C. pseudotuberculosis. A lower antimicrobial activity of the brown propolis extract was identified. Propolis extracts were effective in interfering with the formation of the C. pseudotuberculosis biofilm but had little activity on the consolidated biofilm. In conclusion, propolis extracts are more effective against C. pseudotuberculosis in the planktonic stage, being able to interfere with the formation of bacterial biofilm. However, the action of propolis extracts in a sessile and structured microbial biofilm is reduced.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

et al. 2021

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“…However, biofilm formation is a fairly complex process that cannot be restricted to merely these three main components. Many other factors such as the type of organism, surface, genetic variations, and other environmental factors serve a purpose in biofilm formation ( Steenackers et al, 2016 ; Ponomareva et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Understanding How Staphylococcal Autolysin Domains Interact With Polystyrene Surfaces

et al. 2021

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Biofilms, when formed on medical devices, can cause malfunctions and reduce the efficiency of these devices, thus complicating treatments and serving as a source of infection. The autolysin protein of Staphylococcus epidermidis contributes to its biofilm forming ability, especially on polystyrene surfaces. R2ab and amidase are autolysin protein domains thought to have high affinity to polystyrene surfaces, and they are involved in initial bacterial attachment in S. epidermidis biofilm formation. However, the structural details of R2ab and amidase binding to surfaces are poorly understood. In this study, we have investigated how R2ab and amidase influence biofilm formation on polystyrene surfaces. We have also studied how these proteins interact with polystyrene nanoparticles (PSNPs) using biophysical techniques. Pretreating polystyrene plates with R2ab and amidase domains inhibits biofilm growth relative to a control protein, indicating that these domains bind tightly to polystyrene surfaces and can block bacterial attachment. Correspondingly, we find that both domains interact strongly with anionic, carboxylate-functionalized as well as neutral, non-functionalized PSNPs, suggesting a similar binding interaction for nanoparticles and macroscopic surfaces. Both anionic and neutral PSNPs induce changes to the secondary structure of both R2ab and amidase as monitored by circular dichroism (CD) spectroscopy. These changes are very similar, though not identical, for both types of PSNPs, suggesting that carboxylate functionalization is only a small perturbation for R2ab and amidase binding. This structural change is also seen in limited proteolysis experiments, which exhibit substantial differences for both proteins when in the presence of carboxylate PSNPs. Overall, our results demonstrate that the R2ab and amidase domains strongly favor adsorption to polystyrene surfaces, and that surface adsorption destabilizes the secondary structure of these domains. Bacterial attachment to polystyrene surfaces during the initial phases of biofilm formation, therefore, may be mediated by aromatic residues, since these residues are known to drive adsorption to PSNPs. Together, these experiments can be used to develop new strategies for biofilm eradication, ensuring the proper long-lived functioning of medical devices.

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“…Several environmental factors can affect the biofilm development and its dynamic (Toyofuku et al, 2016); however, the combined effect of multiple environmental factors in biofilm development is poorly addressed (Ponomareva et al, 2018). Most of the in vitro biofilm models are usually restricted to biofilm formation of single species bacteria and in a specific environment (Lebeaux et al, 2013).…”

Section: Factors Affecting Biofilm Formationmentioning

confidence: 99%

Biofilms as a microbial hazard in the food industry: A scoping review

Sharan

Vijay

Dhaka

et al. 2022

Journal of Applied Microbiology

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Biofilms pose a serious public health hazard with a significant economic impact on the food industry. The present scoping review is designed to analyse the literature published during 2001–2020 on biofilm formation of microbes, their detection methods, and association with antimicrobial resistance (if any). The peer‐reviewed articles retrieved from 04 electronic databases were assessed using PRISMA‐ScR guidelines. From the 978 preliminary search results, a total of 88 publications were included in the study. On analysis, the commonly isolated pathogens were Listeria monocytogenes, Staphylococcus aureus, Salmonella spp., Escherichia coli, Bacillus spp., Vibrio spp., Campylobacter jejuni and Clostridium perfringens. The biofilm‐forming ability of microbes was found to be influenced by various factors such as attachment surfaces, temperature, presence of other species, nutrient availability etc. A total of 18 studies characterized the biofilm‐forming genes, particularly for S. aureus, Salmonella spp., and E. coli. In most studies, polystyrene plate and/or stainless‐steel coupons were used for biofilm formation, and the detection was carried out by crystal violet assays and/or by plate counting method. The strain‐specific significant differences in biofilm formation were observed in many studies, and few studies carried out analysis of multi‐species biofilms. The association between biofilm formation and antimicrobial resistance was not clearly defined. Further, viable but non‐culturable form of the foodborne pathogens is posing an unseen (by conventional cultivation techniques) but potent threat to the food safety. The present review recommends the need for carrying out systematic surveys and risk analysis of biofilms in food chain to highlight the evidence‐based public health concerns, especially in regions where microbiological food hazards are quite prevalent.

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Abiotic Environmental Factors Affecting the Formation of Microbial Biofilms

Cited by 23 publications

References 41 publications

Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

Understanding How Staphylococcal Autolysin Domains Interact With Polystyrene Surfaces

Biofilms as a microbial hazard in the food industry: A scoping review

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