ABI5 modulates seed germination via feedback regulation of the expression of the <i>PYR/PYL/RCAR</i> ABA receptor genes

Zhao, Hongyun; Nie, Kaili; Zhou, Huapeng; Yan, Xiaojing; Zhan, Qidi; Zheng, Yuan; Song, Chun‐Peng

doi:10.1111/nph.16713

Cited by 115 publications

(82 citation statements)

References 46 publications

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“…Thus far, MED25 has been implicated in performing various regulatory functions in the hormone signaling pathways (jasmonic acid (JA), abscisic acid (ABA), auxin, and ethylene), biotic and abiotic stress responses and developmental processes (Kazan, 2017). Extensive evidence indicates that MED25 is a master regulator of JA signaling due to the findings of the attenuated expression of JA responsive genes in med25 mutant as well as MED25's direct interaction with various JA‐related TFs, activators, and repressors (Kidd et al, 2009, 2010; Ou et al, 2011; Cevik et al, 2012; Chen et al, 2012; Yang et al, 2015; Wang et al, 2019; You et al, 2019; Zhai and Li, 2019). In addition to playing a role in the JA signaling, MED25 also positively contributes to auxin and ethylene signaling (Yang et al, 2014; Ito et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Mediator tail module subunits MED16 and MED25 differentially regulate abscisic acid signaling in Arabidopsis

Guo

Chong

et al. 2021

JIPB

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MED25 has been implicated as a negative regulator of the abscisic acid (ABA) signaling pathway. However, it is unclear whether other Mediator subunits could associate with MED25 to participate in the ABA response. Here, we used affinity purification followed by mass spectrometry to uncover Mediator subunits that associate with MED25 in transgenic plants. We found that at least 26 Mediator subunits, belonging to the head, middle, tail, and CDK8 kinase modules, were co‐purified with MED25 in vivo. Interestingly, the tail module subunit MED16 was identified to associate with MED25 under both mock and ABA treatments. We further showed that the disruption of MED16 led to reduced ABA sensitivity compared to the wild type. Transcriptomic analysis revealed that the expression of several ABA‐responsive genes was significantly lower in med16 than those in wild type. Furthermore, we discovered that MED16 may possibly compete with MED25 to interact with the key transcription factor ABA INSENSITIVE 5 (ABI5) to positively regulate ABA signaling. Consistently, med16 and med25 mutants displayed opposite phenotypes in ABA response, cuticle permeability, and differential ABI5‐mediated EM1 and EM6 expression. Together, our data indicate that MED16 and MED25 differentially regulate ABA signaling by antagonistically affecting ABI5‐mediated transcription in Arabidopsis.

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Section: Introductionmentioning

confidence: 99%

Mediator tail module subunits MED16 and MED25 differentially regulate abscisic acid signaling in Arabidopsis

Guo

Chong

et al. 2021

JIPB

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“…The ABA signaling pathway mainly relies on OsbZIP transcription factors, including AREBs, ABFs, and ABI5. Among these, ABI5 plays a key role in suppressing ABA-mediated seed germination and post-germination growth in a number of plant species, such as Arabidopsis [13,40,41], rice [42], wheat [43,44], barley [45], and Sorghum [46]. In addition to the roles of these classical bZIP transcriptional factors in the ABA pathway, several other bZIP members are also involved in the ABAmediated regulation of seed dormancy and germination in rice.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination

Zhu¹,

Wang²,

Lu³

et al. 2021

IJMS

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Seed dormancy and germination are key events in plant development and are critical for crop production, and defects in seed germination or the inappropriate release of seed dormancy cause substantial losses in crop yields. Rice is the staple food for more than half of the world’s population, and preharvest sprouting (PHS) is one of the most severe problems in rice production, due to a low level of seed dormancy, especially under warm and damp conditions. Therefore, PHS leads to yield loss and a decrease in rice quality and vitality. We reveal that mutation of OsbZIP09 inhibited rice PHS. Analysis of the expression of OsbZIP09 and its encoded protein sequence and structure indicated that OsbZIP09 is a typical bZIP transcription factor that contains conserved bZIP domains, and its expression is induced by ABA. Moreover, RNA sequencing (RNA-seq) and DNA affinity purification sequencing (DAP-seq) analyses were performed and 52 key direct targets of OsbZIP09 were identified, including OsLOX2 and Late Embryogenesis Abundant (LEA) family genes, which are involved in controlling seed germination. Most of these key targets showed consistent changes in expression in response to abscisic acid (ABA) treatment and OsbZIP09 mutation. The data characterize a number of key target genes that are directly regulated by OsbZIP09 and contribute to revealing the molecular mechanism that underlies how OsbZIP09 controls rice seed germination.

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“…This family has been reported to be involved in ABA-mediated seed germination [ 32 ]. In Arabidopsis , PYR1-PYL1-PYL2-PYL4 [ 31 ], PYL12 [ 50 ], and PYL13 [ 51 ] act as positive regulators of ABA signaling during seed germination and early seedling development. Among these, PYL12 expression was found to be high in mature dry seeds but dropped sharply after germination [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

“…In Arabidopsis , PYR1-PYL1-PYL2-PYL4 [ 31 ], PYL12 [ 50 ], and PYL13 [ 51 ] act as positive regulators of ABA signaling during seed germination and early seedling development. Among these, PYL12 expression was found to be high in mature dry seeds but dropped sharply after germination [ 50 ]. This is consistent with our results, suggesting that significantly downregulated PYL12-like (evm.TU.scaffold_61.29) prevents ABA signal transduction and thereby breaks the dormancy of seeds.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Reveals Roles of Lignin-Modifying Enzymes and Abscisic Acid in the Symbiosis of Mycena and Gastrodia elata

Ren

Zhao

Liu

et al. 2021

IJMS

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Gastrodia elata is a well-known medicinal and heterotrophic orchid. Its germination, limited by the impermeability of seed coat lignin and inhibition by abscisic acid (ABA), is triggered by symbiosis with fungi such as Mycena spp. However, the molecular mechanisms of lignin degradation by Mycena and ABA biosynthesis and signaling in G. elata remain unclear. In order to gain insights into these two processes, this study analyzed the transcriptomes of these organisms during their dynamic symbiosis. Among the 25 lignin-modifying enzyme genes in Mycena, two ligninolytic class II peroxidases and two laccases were significantly upregulated, most likely enabling Mycena hyphae to break through the lignin seed coats of G. elata. Genes related to reduced virulence and loss of pathogenicity in Mycena accounted for more than half of annotated genes, presumably contributing to symbiosis. After coculture, upregulated genes outnumbered downregulated genes in G. elata seeds, suggesting slightly increased biological activity, while Mycena hyphae had fewer upregulated than downregulated genes, indicating decreased biological activity. ABA biosynthesis in G. elata was reduced by the downregulated expression of 9-cis-epoxycarotenoid dioxygenase (NCED-2), and ABA signaling was blocked by the downregulated expression of a receptor protein (PYL12-like). This is the first report to describe the role of NCED-2 and PYL12-like in breaking G. elata seed dormancy by reducing the synthesis and blocking the signaling of the germination inhibitor ABA. This study provides a theoretical basis for screening germination fungi to identify effective symbionts and for reducing ABA inhibition of G. elata seed germination.

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ABI5 modulates seed germination via feedback regulation of the expression of the PYR/PYL/RCAR ABA receptor genes

Cited by 115 publications

References 46 publications

Mediator tail module subunits MED16 and MED25 differentially regulate abscisic acid signaling in Arabidopsis

Mediator tail module subunits MED16 and MED25 differentially regulate abscisic acid signaling in Arabidopsis

Genome-Wide Identification and Expression Analysis of OsbZIP09 Target Genes in Rice Reveal Its Mechanism of Controlling Seed Germination

Transcriptome Reveals Roles of Lignin-Modifying Enzymes and Abscisic Acid in the Symbiosis of Mycena and Gastrodia elata

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