Aberrantly elevated suprabasin in the bone marrow as a candidate biomarker of advanced disease state in myelodysplastic syndromes

Přibyl, Miroslav; Hubáčková, Soňa; Moudrá, Alena; Vancurová, M; Polácková, H; Stopka, Tomáš; Jonášová, Anna; Bokorova, Radka; Fuchs, Ota; Stŕíteský, J; Šalovská, Barbora; Bártek, Jiří; Hodný, Zdeněk

doi:10.1002/1878-0261.12768

Cited by 7 publications

(13 citation statements)

References 52 publications

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“…Multiple studies described aberrant elevation of SBSN in human malignancies [ 19 , 29 , 32 ], but the mechanisms responsible for SBSN upregulation under pathological conditions is not understood. As mentioned, SBSN expression is limited to specific tissues.…”

Section: Regulation Of Sbsn Expressionmentioning

confidence: 99%

“…Importantly, SBSN in ASC-conditioned medium promoted migration of ER-negative breast carcinoma cell line MDA-MB-231 in an estrogen-independent manner [ 30 ]. Note, aberrantly elevated SBSN is observed in the bone marrow, and the peripheral blood of myelodysplastic syndromes (MDS) patients [ 32 ], hence systemic presence of SBSN accompany this disorder.…”

Section: Sbsn In Cancermentioning

confidence: 99%

“…Note, LRG1, a granulocytes-progenitor receptor [ 56 ], was confirmed in neovascularization processes of retinal disease [ 57 ]. Secondly, SBSN expression was shown to be aberrantly elevated by myeloid compartment, predominantly by myeloid-derived suppressor cells (MDSCs) and early-stage MDSCs (eMDSCSs), in bone marrow (BM) of MDS patients [ 32 ]. Importantly, a subgroup of MDS patients with the highest bone marrow (BM) SBSN levels corresponded to a poor prognosis, high-risk group.…”

Section: Sbsn In Cancermentioning

confidence: 99%

“…SBSN expression was therapy-independent, and hence an intrinsic feature of the disease. Importantly, SBSN levels in peripheral blood reflected BM levels of SBSN [ 32 ]. Together with the observation of the presence of SBSN in the pleural fluid of patients suffering from lung adenocarcinoma [ 39 ], MDS represent another human malignancy in which SBSN is proposed as a potential biomarker [ 32 ].…”

Section: Sbsn In Cancermentioning

confidence: 99%

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Suprabasin—A Review

Přibyl

Hodný

Kubíková

2021

Genes

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Among the ~22,000 human genes, very few remain that have unknown functions. One such example is suprabasin (SBSN). Originally described as a component of the cornified envelope, the function of stratified epithelia-expressed SBSN is unknown. Both the lack of knowledge about the gene role under physiological conditions and the emerging link of SBSN to various human diseases, including cancer, attract research interest. The association of SBSN expression with poor prognosis of patients suffering from oesophageal carcinoma, glioblastoma multiforme, and myelodysplastic syndromes suggests that SBSN may play a role in human tumourigenesis. Three SBSN isoforms code for the secreted proteins with putative function as signalling molecules, yet with poorly described effects. In this first review about SBSN, we summarised the current knowledge accumulated since its original description, and we discuss the potential mechanisms and roles of SBSN in both physiology and pathology.

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Section: Regulation Of Sbsn Expressionmentioning

confidence: 99%

Section: Sbsn In Cancermentioning

confidence: 99%

Section: Sbsn In Cancermentioning

confidence: 99%

Section: Sbsn In Cancermentioning

confidence: 99%

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Suprabasin—A Review

Přibyl

Hodný

Kubíková

2021

Genes

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“…Suprabasin, originally identified in mouse and human differentiating keratinocytes 13 , is an epidermal differentiation marker detected in the suprabasal layers of the epithelia of the tongue, stomach and epidermis, indicating a potential role in epidermal differentiation 14 . Suprabasin is highly expressed in several human solid malignancy 15 , 16 , bone marrow cells 17 and tumor endothelium 18 , contributes to cancer progression, invasiveness and metastasis 15 , 16 , 19 , 20 and is hypothesized to be a proto-oncogene candidate of still unknown function 19 . The power of our peptidomic approach for identifying bioactive peptides is illustrated by the discovery of suprabasin-derived bioactive peptides in human plasma.…”

Section: Introductionmentioning

confidence: 99%

Suprabasin-derived bioactive peptides identified by plasma peptidomics

Taguchi

Kodera

Oba

et al. 2021

Sci Rep

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Identification of low-abundance, low-molecular-weight native peptides using non-tryptic plasma has long remained an unmet challenge, leaving potential bioactive/biomarker peptides undiscovered. We have succeeded in efficiently removing high-abundance plasma proteins to enrich and comprehensively identify low-molecular-weight native peptides using mass spectrometry. Native peptide sequences were chemically synthesized and subsequent functional analyses resulted in the discovery of three novel bioactive polypeptides derived from an epidermal differentiation marker protein, suprabasin. SBSN_HUMAN[279–295] potently suppressed food/water intake and induced locomotor activity when injected intraperitoneally, while SBSN_HUMAN[225–237] and SBSN_HUMAN[243–259] stimulated the expression of proinflammatory cytokines via activation of NF-κB signaling in vascular cells. SBSN_HUMAN[225–237] and SBSN_HUMAN[279–295] immunoreactivities were present in almost all human organs analyzed, while immunoreactive SBSN_HUMAN[243–259] was abundant in the liver and pancreas. Human macrophages expressed the three suprabasin-derived peptides. This study illustrates a new approach for discovering unknown bioactive peptides in plasma via the generation of peptide libraries using a novel peptidomic strategy.

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