Aberrant promoter methylation of WIF-1 and SFRP1, 2, 4 genes in mesothelioma

Kohno, Hidekazu; Amatya, Vishwa Jeet; Takeshima, Yukio; Kushitani, Kei; Hattori, Noboru; Kohno, Nobuoki; Inai, Kouki

doi:10.3892/or_00000875

Cited by 18 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cheng et al (42) found that ZIC1 was frequently absent in MPM cell lines, while present in normal mesothelial cell lines. The study used decitabine to demethylate samples or a plasmid expression construct to restore ZIC1 expression, and found that doing this had a similar role in the way of inhibition of the Wnt signaling pathway (62). This suggests ZIC1 functions as a TSG in malignant mesothelioma.…”

Section: Dna Methylationmentioning

confidence: 99%

The Use of Immunohistochemistry, Fluorescence in situ Hybridization, and Emerging Epigenetic Markers in the Diagnosis of Malignant Pleural Mesothelioma (MPM): A Review

et al. 2020

View full text Add to dashboard Cite

Malignant pleural mesothelioma (MPM) is an aggressive asbestos related disease that is generally considered to be difficult to diagnose, stage and treat. The diagnostic process is continuing to evolve and requires highly skilled pathology input, and generally an extensive list of biomarkers for definitive diagnosis. Diagnosis of MPM requires histological evidence of invasion by malignant mesothelial cells often confirmed by various immunohistochemical biomarkers in order to separate it from pleural metastatic carcinoma. Often when invasion of neoplastic mesothelial cells into adjacent tissue is not apparent, further immunohistochemical testing-namely BAP1 and MTAP, as well as FISH testing for loss of p16 (CDKN2A) are used to separate reactive mesothelial proliferation due to benign processes, from MPM. Various combinations of these markers, such as BAP1 and/or MTAP immunohistochemistry alongside FISH testing for loss of p16, have shown excellent sensitivity and specificity in the diagnosis of MPM. Additionally, over the recent years, research into epigenetic marker use in the diagnosis of MPM has gained momentum. Although still in their research stages, various markers in DNA methylation, long non-coding RNA, micro RNA, circular RNA, and histone modifications have all been found to support diagnosis of MPM with generally good sensitivity and specificity. Many of these studies are however, limited by small sample sizes or other study limitations and further research into the area would be beneficial. Epigenetic markers show promise for use in the future to facilitate the diagnosis of MPM.

show abstract

Section: Dna Methylationmentioning

confidence: 99%

The Use of Immunohistochemistry, Fluorescence in situ Hybridization, and Emerging Epigenetic Markers in the Diagnosis of Malignant Pleural Mesothelioma (MPM): A Review

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Macrófagos que fagocitan las fibras de asbesto, pero no pueden digerirlas y producen abundantes especies reactivas de oxígeno. 12…”

Section: Alteraciones Moleculares Inducidas Por El Asbestounclassified

Panorama epigenético del mesotelioma pleural maligno

Álvarez-Moran¹,

Ávila-Sánchez²

2019

NCT Neumología Y Cirugía De Tórax

View full text Add to dashboard Cite

RESUMEN. El mesotelioma pleural maligno es un tumor con una incidencia relativamente baja, en donde gran parte de su carcinogénesis involucra factores epigenéticos que mantienen su heterogeneidad; y en ocasiones, son un blanco terapéutico, o bien un obstáculo a la efectividad en los tratamientos actuales. Esta revisión resume los principales mecanismos de desregulación epigenética involucrados en la patogenia del mesotelioma pleural maligno, tales como la hipermetilación mediada por ADNmetiltransferasas de distintos genes supresores de tumores, así como su relación con la exposición a fibras de asbesto que es el principal factor de riesgo. Su génesis se relaciona a inflamación crónica por radicales libres que culmina en alteraciones cromosómicas, inestabilidad genómica y expresión de genes que promueven la invasión tumoral y angiogénesis. Existen otras vías independientes de metilación que producen silenciamiento génico como el complejo Polycomb y la mutación de la vía SWI/ SNF. Por último, se mencionan otros mecanismos epigenéticos como la hipometilación con pérdida de impronta y activación de genes CG que inducen respuestas inmunológicas, así como la acetilación, desacetilación y desmetilación en el contexto de la cromatina e histonas. Todo lo previo con el objetivo de saber el uso clínico en cuanto al diagnóstico y tratamiento actual epigenético.

show abstract

“…PI3-K and AKT, MAPK signalling, ERK signalling and NF-κB signalling and downstream of receptor tyrosine kinases such as EGFR and FGFR. In addition, WNT signalling might contribute by epigenetic alterations to the cellular signalling and contribute to cellular aberrations (Inai, 2010).…”

Section: Overview Of Methylated Genes and Correspondence To The Literaturementioning

confidence: 99%

Global and gene-specific DNA methylation effects of different asbestos fibres on human bronchial epithelial cells

Öner

Ghosh

Moisse

et al. 2018

Environment International

View full text Add to dashboard Cite

Inhalation exposure to asbestos is associated with lung and pleural diseases in humans and remains a major public health issue worldwide. Human bronchial epithelial cells (16HBE) were exposed to UICC amosite, crocidolite and chrysotile. Cytotoxicity, genotoxicity, global DNA methylation on cytosine residues (using LC-MS/MS) were investigated at different doses (2.5-100 μg/ml). Gene-specific DNA methylation alterations at the whole genome were investigated using a microarray that interrogates >450 thousand CpG sites. Subsequently, gene functional analyses (KEGG pathway, Gene Ontology and functional classification) were performed on genes with differentially methylated gene promoters. At non-cytotoxic doses, global DNA methylation was altered after 24 h exposure to amosite and crocidolite (>2.5 μg/ml). Exposure to amosite and crocidolite (amphibole type asbestos) induced both hypomethylation and hypermethylation at single CpG site and gene promoter levels whereas exposure to chrysotile (serpentine type asbestos) induced hypomethylation at the gene promoter level. Gene functional classification analyses revealed that all types of asbestos fibres induce alterations on GO-clusters i.e. on regulation of Rho-protein signal transduction, nucleus, (e.g. homeobox genes), ATP-binding function and extracellular region (e.g. WNT-group of genes). These differentially methylated genes might contribute to asbestos-related diseases in bronchial cells.

show abstract

Aberrant promoter methylation of WIF-1 and SFRP1, 2, 4 genes in mesothelioma

Cited by 18 publications

References 17 publications

The Use of Immunohistochemistry, Fluorescence in situ Hybridization, and Emerging Epigenetic Markers in the Diagnosis of Malignant Pleural Mesothelioma (MPM): A Review

The Use of Immunohistochemistry, Fluorescence in situ Hybridization, and Emerging Epigenetic Markers in the Diagnosis of Malignant Pleural Mesothelioma (MPM): A Review

Panorama epigenético del mesotelioma pleural maligno

Global and gene-specific DNA methylation effects of different asbestos fibres on human bronchial epithelial cells

Contact Info

Product

Resources

About