Aberrant phenotypes and quantitative antigen expression in different subtypes of canine lymphoma by flow cytometry

Gelain, Maria Elena; Mazzilli, Maria Cristina; Riondato, Fulvio; Marconato, Laura; Comazzi, S.

doi:10.1016/j.vetimm.2007.09.018

Cited by 86 publications

(119 citation statements)

References 16 publications

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“…In the authors' experience, CD4+ T cell lymphoma (TCL) is the most common T‐cell immunophenotype seen in aspirates from canine peripheral lymph nodes and this is consistent with 2 small case series of canine TCL 4, 7. In a very small number of cases with concurrent Kiel cytologic classification, CD4+ TCL were described as small clear cell/T zone, lymphoblastic, and pleomorphic mixed cell/peripheral T‐cell subtypes 3, 4. When lymphoma restricted to the Boxer breed was examined, >80% of cases were T‐cell origin and the majority were of the CD4+ phenotype 5, 8.…”

supporting

confidence: 82%

supporting

confidence: 74%

“…A recent paper examining gene expression profiles in a small number of canine lymphoma cases suggests that both PTCL and LBT have similar gene expression profiles and may represent a continuum of the same disease 26. Work comparing surface phenotype with the Kiel cytologic classification in a small subset of canine T‐cell lymphomas found that 5/8 small clear cell (T zone correlate) and 5/5 pleomorphic mixed small and large (PTCL correlate) tumors expressed surface CD4 4. Interestingly, 5/8 of the small clear cell/T zone cases had dim or absent CD45 expression and one of the small clear cell/T zone cases expressed low levels of CD21.…”

Section: Discussionmentioning

confidence: 99%

“…Despite its expanding diagnostic role in veterinary medicine, there are few flow cytometric descriptions of canine lymphoma 3, 4, 5, 6, 7. In the authors' experience, CD4+ T cell lymphoma (TCL) is the most common T‐cell immunophenotype seen in aspirates from canine peripheral lymph nodes and this is consistent with 2 small case series of canine TCL 4, 7. In a very small number of cases with concurrent Kiel cytologic classification, CD4+ TCL were described as small clear cell/T zone, lymphoblastic, and pleomorphic mixed cell/peripheral T‐cell subtypes 3, 4.…”

mentioning

confidence: 99%

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Flow Cytometric Characterization and Clinical Outcome of CD4+ T‐Cell Lymphoma in Dogs: 67 Cases

Avery

Burton

Bromberek

et al. 2014

Veterinary Internal Medicne

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BackgroundCanine T‐cell lymphoma (TCL) is conventionally considered an aggressive disease, but some forms are histologically and clinically indolent. CD4 TCL is reported to be the most common subtype of TCL. We assessed flow cytometric characteristics, histologic features when available, and clinical outcomes of CD4+ TCL to determine if flow cytometry can be used to subclassify this group of lymphomas.ObjectiveTo test the hypothesis that canine CD4+ T‐cell lymphoma (TCL) is a homogeneous group of lymphomas with an aggressive clinical course.AnimalsSixty‐seven dogs diagnosed with CD4+ TCL by flow cytometry and treated at 1 of 3 oncology referral clinics.MethodsRetrospective multivariable analysis of outcome in canine CD4+ TCL including patient characteristics, treatment, and flow cytometric features.ResultsThe majority of CD4+ TCL were CD45+, expressed low class II MHC, and exhibited an aggressive clinical course independent of treatment regimen (median survival, 159 days). Histologically, CD4+ TCL were classified as lymphoblastic or peripheral T cell. Size of the neoplastic lymphocytes had a modest effect on both PFI and survival in this group. A small number of CD4+ TCL were CD45− and class II MHC high, and exhibited an apparently more indolent clinical course (median survival not yet reached).Conclusions and Clinical ImportanceAlthough the majority of CD4+ TCL in dogs had uniform clinical and flow cytometric features and an aggressive clinical course, a subset had a unique immunophenotype that predicts significantly longer survival. This finding strengthens the utility of flow cytometry to aid in the stratification of canine lymphoma.

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supporting

confidence: 82%

supporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Flow Cytometric Characterization and Clinical Outcome of CD4+ T‐Cell Lymphoma in Dogs: 67 Cases

Avery

Burton

Bromberek

et al. 2014

Veterinary Internal Medicne

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“…CD3 and CD21 coexpression has been detected in dogs with lymphoma. 13 Similarly, a 2008 study 6 also reported a case of lymphoma with the CD3+ CD21+ phenotype, but a similar finding was not reported in other studies of dogs with lymphoid neoplasia. 1,14 In cases of canine lymphoma reported previously, 13 the coexpression of CD3 and CD21 had been shown in nodal lymphocytes from 4 of 59 dogs.…”

Section: Discussionmentioning

confidence: 66%

Immunophenotyping and Gene Rearrangement Analysis in Dogs with Lymphoproliferative Disorders Characterized by Small-Cell Lymphocytosis

Yagihara

Uematsu²,

Koike³

et al. 2009

J VET Diagn Invest

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Abstract. Lymphocytosis caused by neoplastic proliferation of small lymphocytes is occasionally difficult to distinguish by morphological examination from nonneoplastic lymphocytosis. To examine the clinical utility of gene rearrangement analysis for demonstrating neoplastic proliferation of small lymphocytes, gene rearrangement analysis was performed in comparison with immunophenotyping using peripheral lymphocytes in dogs with small lymphocytosis. Thirty-one dogs with small-cell lymphocytosis (8,100-884,300/ml) were enrolled. By immunophenotyping, lymphocytosis of all dogs was suggested to be neoplastic in nature based on the detection of marked expansion of phenotypically homogeneous lymphocytes or the presence of an aberrant antigen-expressing population of lymphocytes. In contrast, gene rearrangement analysis represented clonality in 27 dogs (detection rate of 87%). From the present study, gene rearrangement analysis was considered to be worthwhile to strengthen the evidence of neoplastic proliferation of small lymphocytes when coupled with immunophenotyping and to be a suitable diagnostic substitute if immunophenotyping is not available in clinical practice.

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Flow Cytometry in Hematologic Neoplasia

Tarigo¹,

Seelig²,

Avery³

2022

Schalm's Veterinary Hematology

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Aberrant phenotypes and quantitative antigen expression in different subtypes of canine lymphoma by flow cytometry

Cited by 86 publications

References 16 publications

Flow Cytometric Characterization and Clinical Outcome of CD4+ T‐Cell Lymphoma in Dogs: 67 Cases

Flow Cytometric Characterization and Clinical Outcome of CD4+ T‐Cell Lymphoma in Dogs: 67 Cases

Immunophenotyping and Gene Rearrangement Analysis in Dogs with Lymphoproliferative Disorders Characterized by Small-Cell Lymphocytosis

Flow Cytometry in Hematologic Neoplasia

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