Flow Cytometry in Hematologic Neoplasia

Tarigo, Jaime L.; Seelig, Davis; Avery, Anne C.

doi:10.1002/9781119500537.ch64

Cited by 1 publication

(2 citation statements)

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“…No antibodies against E. canis were found in this dog. Other causes of lymphocytosis include hypoadrenocorticism, which was ruled out as the basal serum cortisol concentration was >55 nmol/L (>2 μg/dL); or thymoma, which is expected to have a mixed or double‐negative CD4 − /CD8 − T‐cell lymphocytosis 22,23 …”

Section: Discussionmentioning

confidence: 99%

“…Other causes of lymphocytosis include hypoadrenocorticism, which was ruled out as the basal serum cortisol concentration was >55 nmol/L (>2 μg/dL); or thymoma, which is expected to have a mixed or double-negative CD4 − /CD8 − T-cell lymphocytosis. 22,23 When CLL is found incidentally, and no significant clinical signs or severe hematologic abnormalities are identified, the initial recommendation is active surveillance of the dog rather than active treatment. 20 Besides the clinical deterioration of the dog, the presence of an abnormal hematologic result is one of the reasons used at our institution to initiate treatment of CLL.…”

Section: Discussionmentioning

confidence: 99%

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Presumed pseudo‐Pelger–Huët anomaly and basophilia secondary to chronic lymphocytic leukemia in a dog

Martínez‐Caro,

Agulla,

Viñeta

et al. 2024

Veterinary Clinical Pathol

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A 10‐year‐old neutered male Maltese dog was presented for an investigation of lymphocytosis. The dog was up‐to‐date on vaccinations and deworming. Physical examination did not reveal any significant abnormalities. A complete blood cell count (CBC) showed mild leukocytosis with moderate lymphocytosis, basophilia, and moderate neutropenia, but no significant left shift or toxic change. Serum biochemistry and urinalysis were unremarkable. All performed tests for infectious agents common in this geographical region were negative. No significant abnormalities were found on abdominal ultrasound examination. Multiparametric flow cytometry of peripheral blood showed a CD8+ T‐cell lymphocytosis, and PCR for antigen receptor rearrangement revealed a clonal expansion of the T‐cell receptor gamma chain genes. A clinical diagnosis of chronic lymphocytic leukemia (CLL) was made, and follow‐up was recommended. On Day 48 post‐presentation, the CBC showed mild non‐regenerative anemia (NRA), moderate leucocytosis due to moderate to marked lymphocytosis, basophilia, and a marked increase in hyposegmented neutrophils with mild toxic change in the absence of neutrophilia or neutropenia. Treatment with chlorambucil and prednisolone was initiated. On Days 87 and 197 post‐presentation, the CBC showed mild NRA, with progressively decreasing numbers of hyposegmented neutrophils. The dog remained without clinical signs. Basophilia and probable pseudo‐Pelger–Huët anomaly were possibly secondary to CLL. To the authors' knowledge, this is the first report of these two hematologic conditions secondary to CLL in dogs. Recognition of a pseudo‐Pelger–Huët anomaly is clinically relevant to avoid misinterpretation as a marked left shift due to severe inflammation and prevent unnecessary urgent therapeutic actions.

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Section: Discussionmentioning

confidence: 99%