Aberrant expression of imprinted genes and their regulatory network in cloned cattle

Gong, Zhijuan; Zhou, Yan; Xu, Min; Cai, Qing; Li, H.; Yan, Jingbin; Wang, J.; Zhang, H.-J.; Fan, Shao‐Hua; Qiao, Yuan; Huang, Sheng; Zeng, Fanyi

doi:10.1016/j.theriogenology.2012.03.037

Cited by 13 publications

(10 citation statements)

References 27 publications

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“…; Gong et al . ). It is suggested that increased birth weight and pathologic changes in abnormal cloned cattle may be caused by altered expression of imprinted genes and genes from related regulatory networks (Gong et al .…”

Section: Current Efficiency and Problems In Scntmentioning

confidence: 97%

Recent progress in bovine somatic cell nuclear transfer

Akagi

Geshi

Nagai

2013

Animal Science Journal

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Bovine somatic cell nuclear transfer (SCNT) embryos can develop to the blastocyst stage at a rate similar to that of embryos produced by in vitro fertilization. However, the full-term developmental rate of SCNT embryos is very low, owing to the high embryonic and fetal losses after embryo transfer. In addition, increased birth weight and postnatal mortality are observed at high rates in cloned calves. The low efficiency of SCNT is probably attributed to incomplete reprogramming of the donor nucleus and most of the developmental problems of clones are thought to be caused by epigenetic defects. Applications of SCNT will depend on improvement in the efficiency of production of healthy cloned calves. In this review, we discuss problems and recent progress in bovine SCNT.

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Section: Current Efficiency and Problems In Scntmentioning

confidence: 97%

Recent progress in bovine somatic cell nuclear transfer

Akagi

Geshi

Nagai

2013

Animal Science Journal

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“…At d 18, the beginning of the implantation window, developmental asynchrony between the embryonic disk and the extraembryonic tissue (EET) was shown to be one reason for arrest at implantation (19). In addition, relatively few differentially expressed genes are detected in cloned fetuses (20,21), and comparison of gene expression in term placentomes collected from pregnancies produced by SNCT, in vitro fertilization, and AI (17) showed that, at later stages of development and at term, placentomes of SCNT-derived pregnancies had an altered transcriptome, regardless of whether pathological morphology was observed (15,17). These studies demonstrated that the abnormal development of the placenta is the major cause of failure of clones to complete pregnancy after implantation.…”

Section: Significancementioning

confidence: 99%

Massive dysregulation of genes involved in cell signaling and placental development in cloned cattle conceptus and maternal endometrium

Biase

Rabel

Guillomot

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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A major unresolved issue in the cloning of mammals by somatic cell nuclear transfer (SCNT) is the mechanism by which the process fails after embryos are transferred to the uterus of recipients before or during the implantation window. We investigated this problem by using RNA sequencing (RNA-seq) to compare the transcriptomes in cattle conceptuses produced by SCNT and artificial insemination (AI) at day (d) 18 (preimplantation) and d 34 (postimplantation) of gestation. In addition, endometrium was profiled to identify the communication pathways that might be affected by the presence of a cloned conceptus, ultimately leading to mortality before or during the implantation window. At d 18, the effects on the transcriptome associated with SCNT were massive, involving more than 5,000 differentially expressed genes (DEGs). Among them are 121 genes that have embryonic lethal phenotypes in mice, cause defects in trophoblast and placental development, and/or affect conceptus survival in mice. In endometria at d 18, <0.4% of expressed genes were affected by the presence of a cloned conceptus, whereas at d 34, ∼36% and <0.7% of genes were differentially expressed in intercaruncular and caruncular tissues, respectively. Functional analysis of DEGs in placental and endometrial tissues suggests a major disruption of signaling between the cloned conceptus and the endometrium, particularly the intercaruncular tissue. Our results support a "bottleneck" model for cloned conceptus survival during the periimplantation period determined by gene expression levels in extraembryonic tissues and the endometrial response to altered signaling from clones. somatic cell nuclear transfer | conceptus | placentation | conceptus-maternal communication I n cattle, as in other mammals, exquisitely orchestrated physiological changes of the conceptus and uterus are necessary for a successful pregnancy. Synchronization of the complex events at the time of implantation relies on the timed release of molecular signals from the conceptus and the endometrium. Embryo-derived IFN-τ (IFNT) is the major signal of pregnancy in cattle, preventing luteolysis and regulating the expression of genes that are responsible for promoting local changes in the endometrium to accommodate the conceptus (1-3). In females, progesterone is the major driver of endometrial changes that prepare the uterus for conceptus implantation (4, 5). In addition to IFNT and progesterone, signaling between the bovine conceptus and the endometrium is bidirectional, and involves several pathways that work concomitantly (6) for the successful establishment of pregnancy.Independent studies have shown that the majority of embryonic losses in cattle occur during the period that spans embryo cleavage until the attachment of the blastocyst to the endometrium (7). The reasons for these losses remain unclear and likely result from several factors, including embryonic lethal genes (8, 9), environmental stressors (7), and endometrial condition (10). Cloning of cattle by somatic cell nuclear transfer ...

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“…Imprinted genes play important roles in embryonic development, placentation, lipid/glucose metabolism, and maternal behavior (Kaneda 2011). Some studies showed disruption of imprinted gene expression in SCNT animals as a consequence of epigenetic defects (Yang et al 2005;Curchoe et al 2009;Hori et al 2010;Suzuki et al 2011;Gong et al 2012;Zhang et al 2016). Therefore, imprinted genes are good indicators in determining whether proper reprogramming occurs in cloned cattle germ cells.…”

Section: Introductionmentioning

confidence: 99%

Proper reprogramming of imprinted and non‐imprinted genes in cloned cattle gametogenesis

Kaneda

Watanabe

Akagi

et al. 2017

Animal Science Journal

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Epigenetic abnormalities in cloned animals are caused by incomplete reprogramming of the donor nucleus during the nuclear transfer step (first reprogramming). However, during the second reprogramming step that occurs only in the germline cells, epigenetic errors not corrected during the first step are repaired. Consequently, epigenetic abnormalities in the somatic cells of cloned animals should be erased in their spermatozoa or oocytes. This is supported by the fact that offspring from cloned animals do not exhibit defects at birth or during postnatal development. To test this hypothesis in cloned cattle, we compared the DNA methylation level of two imprinted genes (H19 and PEG3) and three non-imprinted genes (XIST, OCT4 and NANOG) and two repetitive elements (Satellite I and Satellite II) in blood and sperm DNAs from cloned and non-cloned bulls. We found no differences between cloned and non-cloned bulls. We also analyzed the DNA methylation levels of four repetitive elements (Satellite I, Satellite II, Alpha-satellite and Art2) in oocytes recovered from cloned and non-cloned cows. Again, no significant differences were observed between clones and non-clones. These results suggested that imprinted and non-imprinted genes and repetitive elements were properly reprogramed during gametogenesis in cloned cattle; therefore, they contributed to the soundness of cloned cattle offspring.

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Aberrant expression of imprinted genes and their regulatory network in cloned cattle

Cited by 13 publications

References 27 publications

Recent progress in bovine somatic cell nuclear transfer

Recent progress in bovine somatic cell nuclear transfer

Massive dysregulation of genes involved in cell signaling and placental development in cloned cattle conceptus and maternal endometrium

Proper reprogramming of imprinted and non‐imprinted genes in cloned cattle gametogenesis

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