Aberrant behavior of mouse embryo development after blastomere biopsy as observed through time-lapse cinematography

Ugajin, Tomohisa; Hasegawa, Hisataka; Velayo, Clarissa; Nabeshima, Hiroshi; Yaegashi, Nobuo

doi:10.1016/j.fertnstert.2009.06.056

Cited by 19 publications

(15 citation statements)

References 20 publications

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“…Time-lapse cinematography imaging has rendered more information of human embryonic development and growth behaviour in comparison with conventional practice or intermittent observation. Basically, this has shed more light on the elementary descriptions of embryo development [12] and enables compression of the entire course of early embryo development into a short movie; hence this technique can potentially progress in morphologic and chronologic analyses of dynamic embryo developmental patterns, making an important contribution to ART [13]. The combination of optimal incubation conditions for early developing embryos and a time-lapse embryo monitoring system are certainly of special meaning in terms of gaining complete information of the development of embryos and assessing their quality for transfer and freezing.…”

Section: Discussionmentioning

confidence: 99%

Embryo quality, blastocyst and ongoing pregnancy rates in oocyte donation patients whose embryos were monitored by time-lapse imaging

Cruz¹,

Gadea²,

Garrido

et al. 2011

J Assist Reprod Genet

178

124

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Section: Discussionmentioning

confidence: 99%

Embryo quality, blastocyst and ongoing pregnancy rates in oocyte donation patients whose embryos were monitored by time-lapse imaging

Cruz¹,

Gadea²,

Garrido

et al. 2011

J Assist Reprod Genet

178

124

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show abstract

“…More recently, time-lapse analysis revealed impaired developmental dynamic in both human and mouse BB-embryos, in terms of timing of blastulation, expansion/contraction movements and hatching (Duncan et al 2009, Terada et al 2009, Ugajin et al 2010, Bar-el et al 2016, Cimadomo et al 2016, Yao et al 2016. No information on developmental dynamic following TB has been published so far.…”

Section: General Overviewmentioning

confidence: 99%

Embryo biopsy and development: the known and the unknown

Zacchini¹,

Arena²,

Abramik³

et al. 2017

Reproduction

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Preimplantation genetic diagnosis (PGD) has been introduced in clinical practice as a tool for selecting 'healthy' embryos before their transfer in utero. PGD protocols include biopsy of cleaving embryos (blastomere biopsy (BB)) or blastocysts (trophectoderm biopsy (TB)), followed by genetic analysis to select 'healthy' embryos for transfer in utero. Currently, TB is replacing the use of BB in the clinical practice. However, based on the European Society of Human Reproduction and Embryology Preimplantation Genetic Diagnosis Consortium reports, BB has been used in >87% of PGD cycles for more than 10 years. An exhaustive evaluation of embryo biopsy (both BB and TB) risks and safety is still missing. The few epidemiological studies available are quite controversial and/or are limited to normalcy at birth or early childhood. On the other hand, studies on animals have shown that BB can be a risk factor for impaired development, during both pre-and postnatal life, while little is known on TB. Thus, there is an urgent need of focused researches on BB, as it has contributed to give birth to children for more than 10 years, and on TB, as its application is significantly growing in clinical practice. In this context, the aim of this review is to provide a complete overview of the current knowledge on the short-, medium-and long-term effects of embryo biopsy in the mouse model. Reproduction (2017) 154 R143-R148

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“…The standard morphologic criteria include the addition of cells following biopsy (the occurrence of cleavage following biopsy), the presence or absence of compaction, the formation of blastocysts, and the grading of the embryo [12]. Studies using static evaluation of mouse embryos following blastomere biopsy showed that the procedure resulted in an earlier compaction [13] and yielded evidence of developmental delay in both mouse and human [14,15] embryos. Scott et al reported a significant reduction in implantation potential following cleavage-stage biopsy [4].…”

Section: % Of All Biopsy Procedures Performed In Europe Betweenmentioning

confidence: 99%

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

Bar-El

Kalma

Malcov

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of the study was to explore the effect of blastomere biopsy for preimplantation genetic diagnosis (PGD) on the embryos' dynamics, further cleavage, development, and implantation. Methods The study group included 366 embryos from all PGD treatments (September 2012 to June 2014) cultured in the EmbryoScope™ time-lapse monitoring system. The control group included all intracytoplasmic sperm injection (ICSI) embryos cultured in EmbryoScope™ until day 5 during the same time period (385 embryos). Time points of key embryonic events were analyzed with an EmbryoViewer™. Results Most (88 %) of the embryos were biopsied at ≥8 cells. These results summarize the further dynamic development of the largest cohort of biopsied embryos and demonstrate that blastomere biopsy of cleavage-stage embryos significantly delayed compaction and blastulation compared to the control non-biopsied embryos. This delay in preimplanation developmental events also affected postimplantation development as observed when the dynamics of non-implanted embryos (known implantation data (KID) negative) were compared to those of implanted embryos (KID positive). Conclusion Analysis of morphokinetic parameters enabled us to explore how blastomere biopsy interferes with the dynamic sequence of developmental events. Our results show that biopsy delays the compaction and the blastulation of the embryos, leading to a decrease in implantation.

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Aberrant behavior of mouse embryo development after blastomere biopsy as observed through time-lapse cinematography

Cited by 19 publications

References 20 publications

Embryo quality, blastocyst and ongoing pregnancy rates in oocyte donation patients whose embryos were monitored by time-lapse imaging

Embryo quality, blastocyst and ongoing pregnancy rates in oocyte donation patients whose embryos were monitored by time-lapse imaging

Embryo biopsy and development: the known and the unknown

Blastomere biopsy for PGD delays embryo compaction and blastulation: a time-lapse microscopic analysis

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