Abdominal cystic hydatid disease (CHD): Classification of sonographic appearance and response to treatment

Caremani, Marcello; Benci, Antonio; Maestrini, Romeo; Rossi, Gabriella; Menchetti, Donatella

doi:10.1002/(sici)1097-0096(199611/12)24:9<491::aid-jcu1>3.3.co;2-w

Cited by 9 publications

(12 citation statements)

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“…In addition, sera from patients with schistosomiasis, trichinellosis, ®lariasis and healthy donors were included as controls. Hydatid cysts were classi®ed into seven different types according to Caremani et al (1996). In brief, type I are simple cysts, type II are multiple cysts, type III are cysts with detachment of the wall, type IV are cysts with a mixed pattern, type V are heterogeneous cysts, type VI are hyperechoic cysts and type VII are calci®ed cysts.…”

Section: Blood Samplesmentioning

confidence: 99%

Native and recombinant antigens in the immunodiagnosis of human cystic echinococcosis

Ortona

Riganò

Margutti

et al. 2000

Parasite Immunology

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To evaluate the diagnostic sensitivity and specificity of immunoelectrophoresis (IEP), indirect haemagglutination (IHA), enzyme-linked immunosorbent assay (ELISA) and immunoblotting (IB), we compared their ability in detecting IgG antibodies to a hydatid fluid fraction (HFF) and to native and recombinant antigen B of Echinococcus granulosus. We tested sera from patients who had cystic echinococcosis (CE) grouped according to their type of cysts (n = 204), from patients with other parasitic diseases (n = 21), lung or liver carcinomas (n = 6) or serous cysts (n = 26) and from healthy controls (n = 90). HFF-IB gave the highest sensitivity (80%) followed by ELISA (72%), IHA (54%) and IEP (31%), respectively. The diagnostic sensitivity significantly (P < 0.01) decreased as cysts matured from type I-II to type VII. Recombinant and native antigen B-IB yielded similar sensitivity (74%). A large number of clinically or surgically confirmed CE patients (20%) resulted negative. In these patients' sera, IB to assess the usefulness of the recombinant E. granulosus elongation factor-1 beta/delta in detecting IgE antibodies yielded 33% of positivity. Our findings underline the need to standardize techniques and antigenic preparations and to improve the performance of immunodiagnosis by characterizing new antigens and detecting distinct immunoglobulin classes.

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Section: Blood Samplesmentioning

confidence: 99%

Native and recombinant antigens in the immunodiagnosis of human cystic echinococcosis

Ortona

Riganò

Margutti

et al. 2000

Parasite Immunology

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“…CE was clinically, serologically and ecographically diagnosed. Hydatid cysts were classified in seven different types according to Caremani et al (1996). In brief, type I are simple cysts, type II are multiple cysts, type III are cysts with detachment of wall, type IV are cyst with mixed pattern, type V are heterogeneous cysts, type VI are hyperechoic cysts and type VII are calcified cysts.…”

Section: Blood Samplesmentioning

confidence: 99%

Cloning and expression of a cDNA encoding an elongation factor 1β/δ protein from Echinococcus granulosus with immunogenic activity

Margutti

Ortona

Vaccari

et al. 1999

Parasite Immunology

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A cDNA clone (Eg EF-1beta/delta) of Echinococcus granulosus has been isolated by an expression library screened with immunoglobulin (Ig)E of sera from patients with cystic echinococcosis (CE). The Eg EF-1beta/delta was identified on the basis of sequence homology to the subunits beta or delta of the elongation factor-1. The amino acid sequence deduced from this open reading frame is 244 residues long with a predicted molecular mass of 31 kDa. In Southern blot under high stringent condition, Eg EF-1beta/delta hybridized to genomic DNA of E.granulosus at two bands of 4 and 2.5 Kb. In immunoblotting analysis, the Eg EF-1beta/delta protein shows immunological reactivity with sera from CE patients: 51.7% of sera contained IgE, 41.7% IgG and 18.3% IgG4 specific to the recombinant protein. We identify the Eg EF-1beta/delta by immunoblotting with specific monoclonal antibody both in protoscoleces and in sheep hydatid fluid. The higher percentage of humoral immune response against Eg EF-1beta/delta observed in CE patients with calcified cysts than in patients with active cysts indicates the possible release of the protein in the hydatid fluid after protoscoleces degeneration suggesting the possible use of this antigen in the immunosurveillance of CE. Overall, these findings seem to assign to Eg EF-1beta/delta a key role in the allergic disorders and in the complex host-parasite relationship in CE.

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“…CE patients were selected on the basis of cyst morphology before therapy. All patients had type I and II cysts, which indicate active and progressive disease according to the sonographic classification of Caremani et al [8]. In brief, type I cysts are simple cysts (Ia: overall echo-free, without echoes or internal structures; Ib: echofree but with fine and suspended echoes).…”

Section: Blood Samplesmentioning

confidence: 99%

Cytokine gene expression in peripheral blood mononuclear cells (PBMC) from patients with pharmacologically treated cystic echinococcosis

Riganò

Profumo

Buttari

et al. 1999

Clinical and Experimental Immunology

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The influence of pharmacological treatment on the immune response of patients with Echinococcus granulosus infection was evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) to determine mRNA expression for IL-12 p35, IL-12 p40, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha) and IL-4 in PBMC from 12 patients before and after chemotherapy and from seven uninfected controls. Most patients' PBMC showed measurable amounts of IL-12 p35, IL-4, IFN-gamma and TNF-alpha mRNA in parasite antigen-stimulated and unstimulated cultures. Conversely, IL-12 p40 mRNA was detected almost exclusively in successfully treated patients (86%) after therapy. In these patients semiquantitative analysis of RT-PCR products showed a significant difference between IL-12 p40 mRNA mean levels before and after therapy (P = 0.03 in parasite antigen-stimulated cultures; P = 0.001 in unstimulated cultures). IL-4 mRNA was weakly expressed before therapy and more highly so after treatment in both groups of patients and under both culture conditions; IL-4 mRNA reached its highest level in post-therapy PBMC from patients in whom therapy failed (stimulated cultures). IFN-gamma and TNF-alpha mRNA expression increased in patients who responded to therapy and decreased in patients who did not. In contrast to IL-12 p35, IFN-gamma and TNF-alpha mRNAs, IL-12 p40 and IL-4 mRNAs were detected exclusively in patients, suggesting a close relationship between these two cytokines and cystic echinococcosis. Our findings indicate that chemotherapy influences the immune response, thus determining changes in Th1/Th2 cytokine mRNA patterns, predominantly in IL-12 p40 and IL-4 mRNA expression.

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Abdominal cystic hydatid disease (CHD): Classification of sonographic appearance and response to treatment

Cited by 9 publications

References 0 publications

Native and recombinant antigens in the immunodiagnosis of human cystic echinococcosis

Native and recombinant antigens in the immunodiagnosis of human cystic echinococcosis

Cloning and expression of a cDNA encoding an elongation factor 1β/δ protein from Echinococcus granulosus with immunogenic activity

Cytokine gene expression in peripheral blood mononuclear cells (PBMC) from patients with pharmacologically treated cystic echinococcosis

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