Native and recombinant antigens in the immunodiagnosis of human cystic echinococcosis

Ortona, Elena; Riganò, Rachele; Margutti, Paola; Notargiacomo, S; Ioppolo, S.; Vaccari, Sergio; Barca, Stefano; Buttari, Brigitta; Profumo, Elisabetta; Teggi, Antonella; Siracusano, Alessandra

doi:10.1046/j.1365-3024.2000.00336.x

Cited by 91 publications

(68 citation statements)

References 29 publications

(70 reference statements)

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“…One study (Ortona et al, 2000) highlights the need to standardize techniques and antigenic preparations and to improve the performance of immunodiagnosis by characterizing new antigens and detecting distinct immunoglobulin classes. The diagnostic sensitivity and specificity of IEP, ELISA and IB, in detecting IgG antibodies in patient sera to native and recombinant AgB and a hydatid fluid fraction (HFF) were compared.…”

Section: Methodology In Detecting Antibodiesmentioning

confidence: 99%

Recent Advances in the Immunology and Serological Diagnosis of Echinococcosis

Zhang¹,

Li²,

Lin³

et al. 2012

Serological Diagnosis of Certain Human, Animal and Plant Diseases

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Section: Methodology In Detecting Antibodiesmentioning

confidence: 99%

Recent Advances in the Immunology and Serological Diagnosis of Echinococcosis

Zhang¹,

Li²,

Lin³

et al. 2012

Serological Diagnosis of Certain Human, Animal and Plant Diseases

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“…Antigen B (AgB) was prepared as described elsewhere [24,25], the Em2(G11) antigen was prepared as described by Deplazes et al [15], and the recombinant Em18 antigen was provided by the Institute of Parasitology in Bern, Switzerland [26,27].…”

Section: Defined Antigensmentioning

confidence: 99%

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

et al. 2011

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PURPOSE: Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. METHODS: The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. RESULTS: According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and >99.9% for the diagnosis of CE and AE, respectively. Antigens derived from E. multilocularis metacestodes had sensitivities ranging from 84 to 91% and >99.9% for the diagnosis of CE and AE, respectively. Specificities ranged from 92 to >99.9%. Post-test probabilities for the differential diagnosis of AE from liver neoplasias, CE from cystic liver lesions, and screening for AE in Switzerland were around 95, 86 and 2.2%, respectively. Cross-reactions with antibodies in sera of patients with other parasitic affections (fasciolosis, schistosomosis, amebosis, cysticercosis, and filarioses) did occur at variable frequencies, but could be eliminated through the use of confirmatory testing. CONCLUSIONS: Different metacestode-derived antigens of E. granulosus and E. multilocularis are valuable, widely accessible, and cost-efficient tools for the serological diagnosis of echinococcosis. However, confirmatory testing is necessary, due to the lack of species specificity and the occurrence of cross-reactions to other helminthic diseases. Abstract Purpose Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. Methods The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. Results According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and [99.9% for the diagnosis of CE and AE, respectively. Antigens derived from...

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“…9 Post-treatment monitoring of CE patients with such antigens has been of only limited help in prognostically classifying the status of a CE patient after treatment, and serological results allowed the discrimination of cured and non-cured status of CE only retrospectively. This could be due to the temporally delayed reaction of antibody concentrations to clinical changes, 10 in addition to the persistence of specific antibodies for several years after recovery.…”

Section: Introductionmentioning

confidence: 99%

“…17 Most of these studies preliminarily documented a high diagnostic performance of the recombinant antigens. 9,[14][15][16]18,19 Alternatively, synthetic peptides including p176, 20,21 derived from the antigen B sequence, and the 89-122, which mimics an epitope of Ag5, 22 also provided interesting findings. Unfortunately, these recombinant antigens and synthetic peptides were not (or only poorly) investigated for their prognostic value in the serological follow-up of CE.…”

Section: Introductionmentioning

confidence: 99%

Isolation and molecular characterization of recombinant Echinococcus granulosus P29 protein (recP29) and its assessment for the post-surgical serological follow-up of human cystic echinococcosis in young patients

Nouir

Gianinazzi

Gorcii

et al. 2009

Transactions of the Royal Society of Tropical Medicine and Hygi

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SummaryWe synthesized recombinant Echinococcus granulosus protoscolex recP29 antigen to be preliminarily assessed by ELISA and immunoblotting. RecP29-serology was carried out on 54 young patients with cystic echinococcosis (CE). Patients were classified into either cured (CCE) (n = 40) or non-cured (NCCE) (n = 14) CE patients. RecP29 ELISA showed a gradual decrease of antibody concentrations in all CCE cases that were initially (before treatment) seropositive to this antigen (25 out of 40) or that seroconverted following treatment. A complete seronegativity was reached within 3 years post-surgery in all of these cases. Conventional HCF ELISA yielded seronegativity in only 10% of initially recP29-seropositive CCE patients (P = 0.086). Likewise, recP29 immunoblotting yielded seronegativity in 93% of 29 out of 40 initially recP29-immunoblot-positive CCE patients after 3 years follow-up, compared with 72% in the HCF immunoblotting (P = 0.060). Eleven out of 14 NCCE patients were initially positive by recP29 ELISA, and 10 out of these maintained a marked anti-recP29 antibody reactivity until the endpoint of the follow-up period. All 14 NCCE cases were initially seropositive by recP29 immunoblotting, and 13 cases remained seropositive until the end of the study. Thus, recombinant P29 protein appears prognostically useful for monitoring those post-surgical CE cases with an initial seropositivity to this marker.

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Native and recombinant antigens in the immunodiagnosis of human cystic echinococcosis

Cited by 91 publications

References 29 publications

Recent Advances in the Immunology and Serological Diagnosis of Echinococcosis

Recent Advances in the Immunology and Serological Diagnosis of Echinococcosis

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

Isolation and molecular characterization of recombinant Echinococcus granulosus P29 protein (recP29) and its assessment for the post-surgical serological follow-up of human cystic echinococcosis in young patients

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