ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas

Marques‐Santos, Luis Fernando; Hégaret, Hélène; Lima-Santos, Leonardo; Queiroga, Fernando Ramos; Silva, Patrícia Mirella da

doi:10.1016/j.fsi.2017.09.014

Cited by 12 publications

(5 citation statements)

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“…In the same way, antimicrobial peptides were found within granular haemocytes in the scallop N. subnodosus (97). ATPbinding cassette (ABC) proteins associated with the multixenobiotic resistance were observed expressed differently between haemocyte types in mussels and oysters (281,282). Cathepsin L gene expression, involved in the inflammatory response, was strongly associated with the number of circulating granulocytes in C. gigas (283).…”

Section: Immune Molecules and Pathwaysmentioning

confidence: 88%

Bivalve Haemocyte Subpopulations: A Review

et al. 2022

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Bivalve molluscs stand out for their ecological success and their key role in the functioning of aquatic ecosystems, while also constituting a very valuable commercial resource. Both ecological success and production of bivalves depend on their effective immune defence function, in which haemocytes play a central role acting as both the undertaker of the cellular immunity and supplier of the humoral immunity. Bivalves have different types of haemocytes, which perform different functions. Hence, identification of cell subpopulations and their functional characterisation in immune responses is essential to fully understand the immune system in bivalves. Nowadays, there is not a unified nomenclature that applies to all bivalves. Characterisation of bivalve haemocyte subpopulations is often combined with 1) other multiple parameter assays to determine differences between cell types in immune-related physiological activities, such as phagocytosis, oxidative stress and apoptosis; and 2) immune response to different stressors such as pathogens, temperature, acidification and pollution. This review summarises the major and most recent findings in classification and functional characterisation of the main haemocyte types of bivalve molluscs.

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Section: Immune Molecules and Pathwaysmentioning

confidence: 88%

Bivalve Haemocyte Subpopulations: A Review

et al. 2022

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“…It has notably been detected in numerous marine species, including bivalves. In oysters, the involvement of Abcb1 has been hypothesised in a molecular detoxification system through the transport of a variety of molecules and xenobiotics across the cell membrane [ 39 ]. Interestingly, in C. gigas , ATP-binding cassette members might be responsible for the transportation of pigment-related substrate that influences pigmentation [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

Tracing key genes associated with the Pinctada margaritifera albino phenotype from juvenile to cultured pearl harvest stages using multiple whole transcriptome sequencing

et al. 2020

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Background Albino mutations are commonly observed in the animal kingdom, including in bivalves. In the black-lipped pearl oyster Pinctada margaritifera, albino specimens are characterized by total or partial absence of colouration resulting in typical white shell phenotype expression. The relationship of shell colour with resulting cultured pearl colour is of great economic interest in P. margaritifera, on which a pearl industry is based. Hence, the albino phenotype provides a useful way to examine the molecular mechanisms underlying pigmentation. Results Whole transcriptome RNA-sequencing analysis comparing albino and black wild-type phenotypes at three stages over the culture cycle of P. margaritifera revealed a total of 1606, 798 and 187 differentially expressed genes in whole juvenile, adult mantle and pearl sac tissue, respectively. These genes were found to be involved in five main molecular pathways, tightly linked to known pigmentation pathways: melanogenesis, calcium signalling pathway, Notch signalling pathway, pigment transport and biomineralization. Additionally, significant phenotype-associated SNPs were selected (N = 159), including two located in the Pif biomineralization gene, which codes for nacre formation. Interestingly, significantly different transcript splicing was detected between juvenile (N = 1366) and adult mantle tissue (N = 313) in, e.g., the tyrosinase Tyr-1 gene, which showed more complex regulation in mantle, and the Notch1 encoding gene, which was upregulated in albino juveniles. Conclusion This multiple RNA-seq approach provided new knowledge about genes associated with the P. margaritifera albino phenotype, highlighting: 1) new molecular pathways, such as the Notch signalling pathway in pigmentation, 2) associated SNP markers with biomineraliszation gene of interest like Pif for marker-assisted selection and prevention of inbreeding, and 3) alternative gene splicing for melanin biosynthesis implicating tyrosinase.

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“…Calcein-AM crosses the cell plasma membrane and accumulates in the cytoplasm as fluorescent calcein after undergoing the action of esterases that remove the AM group. Calcein-AM is a substrate for ABC transporters, which expel it from the cell; therefore, its accumulation in the cytoplasm is inversely proportional to the activity of the transporters (Marques-Santos et al, 2017).…”

Section: Hemocyte Responses To O Cf Ovatamentioning

confidence: 99%

“…These proteins were associated to MXR phenotype, conferring resistance to several pollutants, from anthropogenic contaminants to biological toxins (Corsi and Marques-Santos, 2018). The activity of these transporters was characterized in the hemocytes of C. gasar (Marques-Santos et al, 2017), but little is known about the role of phycotoxin in the activity of ABC transporters in hemocytes.…”

Section: Studies On Hemograms Of Bivalves Exposed To Microalgae Of Th...mentioning

confidence: 99%

“…This phenotype (MXR) acts by decreasing intracellular concentrations of xenobiotics below their toxic level (Bard, 2000). The presence of ABC transporters has been demonstrated in several bivalves, such as the oysters C. virginica (Keppler and Ringwood, 2001), C. gigas and C. gasar (Marques-Santos et al, 2017), mussels Mytilus californianus, M. edulis and M. galloprovincialis (Della Torre et al, 2014;Eufemia and Epel, 2000;Minier et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

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