ABC transporters involved in the transport of plant secondary metabolites

Yazaki, Kazufumi

doi:10.1016/j.febslet.2005.12.009

Cited by 341 publications

(201 citation statements)

References 111 publications

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“…For example, cluster 27 (septum) contained genes encoding transporters of mineral nutrients, organic macromolecules, and the hormone auxin. There was a particular abundance of ATP-binding cassette transporters in cluster 28 (pericarp), a class of transporter associated with the transport of secondary metabolites (Yazaki, 2006). Their presence in the pericarp, therefore, may be related to secondary metabolite production in this tissue (see cluster 30 above).…”

Section: Stage-dependent Expression Clustersmentioning

confidence: 99%

Comprehensive Tissue-Specific Transcriptome Analysis Reveals Distinct Regulatory Programs during Early Tomato Fruit Development

Pattison

Csukasi²,

Zheng³

et al. 2015

Plant Physiol.

124

135

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Fruit formation and early development involve a range of physiological and morphological transformations of the various constituent tissues of the ovary. These developmental changes vary considerably according to tissue type, but molecular analyses at an organ-wide level inevitably obscure many tissue-specific phenomena. We used laser-capture microdissection coupled to high-throughput RNA sequencing to analyze the transcriptome of ovaries and fruit tissues of the wild tomato species Solanum pimpinellifolium. This laser-capture microdissection-high-throughput RNA sequencing approach allowed quantitative global profiling of gene expression at previously unobtainable levels of spatial resolution, revealing numerous contrasting transcriptome profiles and uncovering rare and cell type-specific transcripts. Coexpressed gene clusters linked specific tissues and stages to major transcriptional changes underlying the ovary-to-fruit transition and provided evidence of regulatory modules related to cell division, photosynthesis, and auxin transport in internal fruit tissues, together with parallel specialization of the pericarp transcriptome in stress responses and secondary metabolism. Analysis of transcription factor expression and regulatory motifs indicated putative gene regulatory modules that may regulate the development of different tissues and hormonal processes. Major alterations in the expression of hormone metabolic and signaling components illustrate the complex hormonal control underpinning fruit formation, with intricate spatiotemporal variations suggesting separate regulatory programs.

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Section: Stage-dependent Expression Clustersmentioning

confidence: 99%

Comprehensive Tissue-Specific Transcriptome Analysis Reveals Distinct Regulatory Programs during Early Tomato Fruit Development

Pattison

Csukasi²,

Zheng³

et al. 2015

Plant Physiol.

124

135

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“…A homologous gene encoding a protein of the pleiotropic drug resistance (PDR) subfamily of ATP-binding cassette (ABC) transporters was found to be induced in grapevine by P. viticola. These ABC transporters mediate the translocation of a diverse array of compounds across biological membranes, including toxins, drugs, glutathione conjugates, peptides and secondary metabolites (Yazaki 2006). There is also increasing evidence for the involvement of ABC transporters in defence.…”

Section: Defence-related Genesmentioning

confidence: 99%

Identification of genes expressed during the compatible interaction of grapevine with Plasmopara viticola through suppression subtractive hybridization (SSH)

Legay¹,

Marouf²,

Berger³

et al. 2010

The Downy Mildews - Biology, Mechanisms of Resistance and Population Ecology

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Grapevine (Vitis vinifera) is the most widely cultivated and economically important fruit crop, but is susceptible to a large number of diseases. Downy mildew, caused by the obligate biotrophic oomycete pathogen Plasmopara viticola, is a common disease present in all regions where vines are cultivated. We used suppression subtractive hybridization (SSH) to generate two cDNA libraries enriched for transcripts induced and repressed, respectively, in the susceptible grapevine cultivar Chasselas 24 h after inoculation with P. viticola. Differential screening on glass slide microarrays yielded over 800 putative genes that were upregulated in response to P. viticola infection and over 200 that were down-regulated. One hundred and ninety four of these, were sequenced, identified and functionally categorised. Transcript abundance of twelve genes over a 48 h time course was examined by reverse transcriptase quantitative real-time PCR (RT-qPCR). Ten of these genes were induced/enhanced by P. viticola challenge, confirming the results of the SSH. The vast majority of the genes identified are related to defence. Interestingly, many genes involved in photosynthesis were down-regulated.

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“…The intracellular trafficking system is responsible for the transport of most macromolecules within the cell and may be essential for the transport of secondary metabolites between cell compartments and the delivery of newly synthesized secondary metabolites to their final destinations and/or secretion out of the cell (Grotewold, 2004;Yazaki, 2006). In addition, the intracellular vesicle trafficking network of plants seems to be linked to many signal transduction pathways and may be involved in the general response to environmental stresses (e.g.…”

Section: Gene Expression Patternsmentioning

confidence: 99%

Herbivore-induced defence response in the brown seaweedFucus vesiculosus(Phaeophyceae): temporal pattern and gene expression

Flöthe

Molis

Kruse

et al. 2014

European Journal of Phycology

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This study aimed to reveal the dynamics of inducible anti-herbivory traits in the brown seaweed Fucus vesiculosus, in response to grazing by the isopod Idotea baltica. As the molecular basis of defence induction in seaweeds is poorly understood, a transcriptomic approach was used to gain insight into cellular processes underlying defence induction and thus promote the mechanistic understanding of anti-herbivory responses in seaweeds. In a 27 day feeding-assayed induction experiment, temporal patterns of the induced anti-herbivory resistance of F. vesiculosus were assessed under laboratory conditions. Feeding assays were performed at three day intervals, using fresh and reconstituted food. Microarray hybridizations investigating the expression of genes 3 days before, as well as at the same time as, feeding assays revealed a decreased palatability of previously grazed F. vesiculosus pieces compared with non-grazed control pieces. Despite permanent exposure to grazers, F. vesiculosus palatability varied over time. Non-grazed F. vesiculosus pieces were significantly preferred to grazed pieces after 18 and again after 27 days of previous grazing, while their relative palatability for isopods was comparable at all other times during the experiment. Relative to controls, 562/402 genes were ≥ 1.5-fold up-/down-regulated in seaweed pieces that were grazed for 18 days, i.e. when induction of defences was detected in feeding assays. Massive reprogramming of the regulatory expression orchestra (translation, transcription) as well as up-regulation of genes involved in lipid and carbohydrate metabolism, intracellular trafficking, defence and stress response was found. At the same time, down-regulation of photosynthesis was observed in grazed seaweed pieces. This study suggests a high level of temporal variability in induced anti-herbivory traits of F. vesiculosus and reveals increased expression of genes with putative defensive functions in conjunction with the reallocation of resources from primary to secondary metabolism.

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ABC transporters involved in the transport of plant secondary metabolites

Cited by 341 publications

References 111 publications

Comprehensive Tissue-Specific Transcriptome Analysis Reveals Distinct Regulatory Programs during Early Tomato Fruit Development

Comprehensive Tissue-Specific Transcriptome Analysis Reveals Distinct Regulatory Programs during Early Tomato Fruit Development

Identification of genes expressed during the compatible interaction of grapevine with Plasmopara viticola through suppression subtractive hybridization (SSH)

Herbivore-induced defence response in the brown seaweedFucus vesiculosus(Phaeophyceae): temporal pattern and gene expression

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