Abstract:Abscisic acid (ABA) inhibited embryogenesis in anther culture of Brussels sprouts. This was accompanied by enhanced ethylene production during the first half of the anther culture period followed by a reduction in ethylene during the latter half, when compared to anthers not treated with ABA. The enhancement of ethylene production by ABA 6 h and 48 h after the start of the culture period was counteracted by the ethylene biosynthesis inhibitor aminoethoxyvinylglycine (AVG). Both AVG and the ethylene antagonist … Show more
“…Our experience in using colchicine in rice anther culture (Chu and Crough an 1989) has indicated that more diploid plants can be produced by expending the same effort on plating additional anthers, rather than utilizing colchicine treatments. Several factors affect the success of anther culture, especially the genotype of the plant from which anthers are obtained (Bajaj 1983;Chu and Croughan 1989) and the composition of the nutrient media (Gaillard et al 1991;Biddington et al 1993;Hoekstra et al 1993). Other factors include the condition of the donor plant, developmental stage of the pollen, and thermal shock pretreatment of the anthers (Bajaj 1983).…”
“…Our experience in using colchicine in rice anther culture (Chu and Crough an 1989) has indicated that more diploid plants can be produced by expending the same effort on plating additional anthers, rather than utilizing colchicine treatments. Several factors affect the success of anther culture, especially the genotype of the plant from which anthers are obtained (Bajaj 1983;Chu and Croughan 1989) and the composition of the nutrient media (Gaillard et al 1991;Biddington et al 1993;Hoekstra et al 1993). Other factors include the condition of the donor plant, developmental stage of the pollen, and thermal shock pretreatment of the anthers (Bajaj 1983).…”
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