Experimental haploidy in Brassica species

Palmer, Constantine E.; Keller, W. A.; Arnison, Paul G.

doi:10.1007/978-94-017-1858-5_9

Cited by 32 publications

(13 citation statements)

References 97 publications

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“…Similar genotype dependence for microspore embryogenesis has been reported by Hiramatsu et al (1995), Lionneton et al (2001) (2005). Genotypic variation for haploid embryo development has been observed in several other Brassica species (Palmer et al 1996) and microspore embryogenic ability has been reported to be highly heritable in B. napus and B. campestris (Zhang and Takahata 2001). The present results also reveal a significant influence of bud size selection against a wider, nonspecific, bud size range of 2-4 mm in B. juncea microspore culture (Hiramatsu et al 1995;Lionneton et al 2001).…”

Section: Discussionsupporting

confidence: 57%

“…The exploitation of microspore culture to produce doubled haploids (DH) may compress the breeding cycle for biotechnological or conventional crop improvement programs. Several Brassica species including Indian mustard have been reported to produce androgenic haploids (Palmer et al 1996). However, the success of this technique as a breeding tool has been limited for B. juncea mainly due to the low frequency of microspore embryogenesis and inefficient regeneration of microspore embryos into doubled haploid plants (Prem et al 2004;Babbar et al 2004).…”

Section: Introductionmentioning

confidence: 98%

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Activated charcoal induced high frequency microspore embryogenesis and efficient doubled haploid production in Brassica juncea

Prem

Gupta

Sarkar³

et al. 2008

Plant Cell Tiss Organ Cult

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Three Indian Brassica juncea cultivars were studied for embryogenic response of microspores, microspore embryo regeneration, ploidy assessment of microspore-derived plants and their diploidization. Genotype dependence for microspore totipotency was observed and a significant effect of genotype by bud size selection was established. The addition of activated charcoal in NLN medium containing 13% (w/v) sucrose and 10 lM silver nitrate resulted in a fourfold increase in microspore embryogenesis, ranging from 100 to 405 embryos per Petri dish corresponding to 2,700-10,935 embryos per 100 buds. Conversion/germination of embryos produced in presence or absence of activated charcoal was similar but air-drying of microspore embryos was essential. Incubation of microspore embryos at 4 ± 1°C for 10 days in dark resulted in 82.3% conversion. The majority of plants produced from these embryos was haploid. Treating microsporederived plants at the 3-4 leaf growth stage with 0.34% colchicine for 2-3 h resulted in greatest survival (70%) and chromosome doubling (75%) frequencies. Doubled haploid plants were self-pollinated and grown to maturity under field conditions.

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Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 98%

Activated charcoal induced high frequency microspore embryogenesis and efficient doubled haploid production in Brassica juncea

Prem

Gupta

Sarkar³

et al. 2008

Plant Cell Tiss Organ Cult

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“…(Palmer et al 1996a). Baillie et al (1992) working with B. campestris and Ferrie et al (1999) studying B. oleracea both demonstrated that culture with 17 % sucrose for 48 h followed by a media change to 10 % sucrose increased the frequency of microspore embryogenesis.…”

Section: Osmoticummentioning

confidence: 93%

“…Here, with the exception of two genotypes (BJ-99 and BJ-7), the decrease from 17 % sucrose during the first 48 h to 10 % thereafter favoured an increase in embryo production. Sucrose in high concentrations can act as an osmotic stress for embryo induction (Palmer et al 1996a). The latter authors suggested that it would be better to discriminate between the osmotic and nutritional roles of sucrose in order to better exploit the carbohydrate role in the embryo formation.…”

Section: Osmoticummentioning

confidence: 99%

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Haploids in the Improvement of Crucifers

Friedt

Zarhloul

Haploids in Crop Improvement II

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Doubled Haploids: A Powerful Biotechnological Tool for Genetic Enhancement in Oilseed Brassicas

Prem

Gupta

Agnihotri³

Plant Biotechnology and Molecular Markers

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Abstract:The review presents the detailed advantages of using doubled haploids for crop improvement programs. It also elaborates the use of the doubled haploid technique in terms of its conjugation with other biotechnological approaches. The hall mark of doubled haploid technique lies in versatile potential of its use in various breeding programs and its ability to compress the time taken for breeding a desired genotype in comparison to conventional breeding methods. The factors affecting the production of haploids/doubled haploids have been discussed in the light of relevant research undertaken towards genetic enhancement for value addition in oilseed brassicas.

show abstract

Experimental haploidy in Brassica species

Cited by 32 publications

References 97 publications

Activated charcoal induced high frequency microspore embryogenesis and efficient doubled haploid production in Brassica juncea

Activated charcoal induced high frequency microspore embryogenesis and efficient doubled haploid production in Brassica juncea

Haploids in the Improvement of Crucifers

Doubled Haploids: A Powerful Biotechnological Tool for Genetic Enhancement in Oilseed Brassicas

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