ABA mediates development-dependent anthocyanin biosynthesis and fruit coloration in Lycium plants

Li, Gen; Zhao, Jianhua; Qin, Beibei; Yin, Yue; An, Wei; Mu, Zixin; Cao, Yang

doi:10.1186/s12870-019-1931-7

Cited by 69 publications

(36 citation statements)

References 46 publications

(70 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different fruits have different optimized conditions for VIGS. For Lycium plants, the optimized conditions included an OD600 of 1.0, with the cells incubated at room temperature for 3-4 h. The pTRV1 and pTRV2 solutions were mixed at a ratio of 1:1 for injection [30]. In tomato, the OD600 was adjusted to 0.5 or 1.0 for all of the vectors, and the resuspended cells with pTRV1 and pTRV2 were then combined at a ratio of 1:1 and kept for more than 10 d in a plastic tray [8,31].…”

Section: Discussionmentioning

confidence: 99%

Optimization of a virus-induced gene silencing system and functional elucidation of MaBAM9b in postharvest banana fruits

liu

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

Abstract Background: The genetic basis of metabolic pathways that operate during fruit ripening needs to be understood before the nutritional value of the banana can be improved. The banana is a typical starch conversion fruit, and β-amylase is a key enzyme that may play an important role in starch degradation during the ripening process. Musa acuminata β-amylase 9b (MaBAM9b) is closely related to starch degradation. However, its exact function in starch degradation has not been demonstrated in banana. Stable genetic transformation to identify gene function is time- and energy-consuming. Thus, an efficient and rapid method is needed for functional identification. Virus-induced gene silencing (VIGS) is a reverse-genetics method based on RNA-mediated antiviral plant defense that has been used to rapidly identify gene functions in plants. The aim of this study is to optimize a VIGS system and functional elucidation of MaBAM9b in postharvest banana fruits. Results: Using 0.5% iodine-potassium-iodide (I2-KI) staining for 150 s, we found that 1:3 TRV1:TRV2-MaBAM9b cultivated at 30 mmHg for 30 s to an optical density (OD) of 0.8 at 600 nm, and kept on Murashige & Skoog (MS) media for 5 days produced the best silencing results. Under these conditions, the total starch content was greatly increased, whereas the β-amylase activity, soluble sugar content, and expression of endogenous MaBAM9b greatly decreased. Conclusions: The system described here is particularly useful for studying genes and networks involved in starch conversion in fruit, which alone would not produce a visual phenotype. This system will provide a platform for functional genomics and fruit quality improvement in the banana.

show abstract

Section: Discussionmentioning

confidence: 99%

Optimization of a virus-induced gene silencing system and functional elucidation of MaBAM9b in postharvest banana fruits

liu

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The amounts of anthocyanins in Lycium fruit extracts were determined spectrophotometrically as described by Li G. et al. (2019) .…”

Section: Methodsmentioning

confidence: 99%

“…Endogenous ABA was extracted from Lycium fruits according to the method described by Li G. et al. (2019) , and ABA content was assayed using a Phytodetek Immunoassay Kit (PDK 09347/0096, Agdia, USA) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

“…However, basic research on the mechanisms behind the regulation of Lycium fruit ripening is very scarce. We recently demonstrated that phytohormone ABA enhanced Lycium fruit coloration/ripening by promoting anthocyanin biosynthesis ( Li G. et al., 2019 ), at both genetic and pharmacological levels. In contrast, in the present work, we further found that LbNR -derived NO inhibited Lycium fruit coloration by suppressing anthocyanin de novo biosynthesis as well as by redirecting the flavonoid biosynthetic pathway from anthocyanin to proanthocyanidin (PA) production.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

LbNR-Derived Nitric Oxide Delays Lycium Fruit Coloration by Transcriptionally Modifying Flavonoid Biosynthetic Pathway

Qin

et al. 2020

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Anthocyanin-derived fleshy fruit pigmentation has become an excellent system for studying the regulatory network underlying fruit ripening and quality. The transcriptional control of anthocyanin biosynthesis by MYB-bHLH-WDR complexes has been well established, but the intermediate signals through which the environmental or developmental cues regulate these transcription factors remain poorly understood. Here we found that nitric oxide (NO) production during Lycium fruit ripening decreased progressively presenting a negative relationship with anthocyanins. After cloning of the nitric reductase (NR) gene from Lycium barbarum (LbNR) plants, we demonstrated that LbNR-derived NO partially inhibited anthocyanin biosynthesis but enhanced proanthocyanidin (PA) accumulation, and delayed fruit coloration. Application of the NO donor, sodium nitroprusside (SNP), produced a similar effect. The endogenous or exogenous NO downregulated the transcripts both of the regulatory genes and the structural genes that related to anthocyanin biosynthesis, while upregulated both of those genes that related to PA biosynthesis. Given there is a significant negative relationship between the levels of anthocyanins and PAs during Lycium fruit ripening, NO not only inhibited anthocyanin de novo biosynthesis but redirected the flavonoid biosynthetic pathway from anthocyanins to PA production. Two types of LrMYB transcription factors of opposite nature, namely anthocyanin-specific and PA-specific, which belong to the R2R3-MYB subfamily and 1R-MYB subfamily, respectively, were identified from L. ruthenicum fruits. It was further found that NO acts by antagonizing the ABA signaling, a phytohormone we have previously shown playing a positive role in Lycium fruit coloration. Our results provided particularly novel information about NO-ABAanthocyanin interplay during Lycium fruit development and ripening, which may fill a gap between the developmental cues and the transcriptional regulation of anthocyanin biosynthesis.

show abstract

“…In the metabolic pathway of flavonoids, including cell wall component synthesis, biosynthesis of glucosinolates, other primary and secondary metabolic reactions, they also play an important role in metabolic regulation [17][18][19][20]. Studies have shown that MYB genes can be induced by environmental factors, such as ABA (abscisic acid), SA (salicylic acid), jasmonate (JA), and others [21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Two responses to MeJA induction of R2R3-MYB transcription factors regulate flavonoid accumulation in Glycyrrhiza uralensis Fisch

Chen

Wang

et al. 2020

PLoS ONE

View full text Add to dashboard Cite

Flavonoids are key components of licorice plant that directly affect its medicinal quality. Importantly, the MYB family of transcription factors serves to regulate the synthesis of flavonoids in plants. The MYB transcription factors represent one of the largest families of transcription factors in plants and play important roles in the process of plant growth and development. MYB gene expression is induced by a number of plant hormones, including the lipid-based hormone jasmonate (JA). Methyl jasmonate (MeJA) is an endogenous plant growth regulator that can induce the JA signaling pathway, which functions to regulate the synthesis of secondary metabolites, including flavonoids. In this study, MeJA was added to licorice cell suspensions, and RNA-seq analysis was performed to identify the differentially expressed genes. As a result, the MYB transcription factors GlMYB4 and GlMYB88 were demonstrated to respond significantly to MeJA induction. Subsequently, the GlMYB4 and GlMYB88 protein were shown to localize to the cell nucleus, and it was verified that GlMYB4 and GlMYB88 could positively regulate the synthesis of flavonoids in licorice cells. Overall, this research helps illustrate the molecular regulation of licorice flavonoid biosynthesis induced by MeJA.

show abstract

ABA mediates development-dependent anthocyanin biosynthesis and fruit coloration in Lycium plants

Cited by 69 publications

References 46 publications

Optimization of a virus-induced gene silencing system and functional elucidation of MaBAM9b in postharvest banana fruits

Optimization of a virus-induced gene silencing system and functional elucidation of MaBAM9b in postharvest banana fruits

LbNR-Derived Nitric Oxide Delays Lycium Fruit Coloration by Transcriptionally Modifying Flavonoid Biosynthetic Pathway

Two responses to MeJA induction of R2R3-MYB transcription factors regulate flavonoid accumulation in Glycyrrhiza uralensis Fisch

Contact Info

Product

Resources

About