Two responses to MeJA induction of R2R3-MYB transcription factors regulate flavonoid accumulation in Glycyrrhiza uralensis Fisch

Li, Yali; Chen, Xiuli; Wang, Jiaqi; Guang-ping, Zhang; Wang, Lu; Li, Xueshuang

doi:10.1371/journal.pone.0236565

Cited by 24 publications

(13 citation statements)

References 61 publications

(74 reference statements)

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“…In Salvia miltiorrhiza , SmMYB1 enhances the biosynthesis of phenolic acid by increasing the expression of CYP98A14 , CHI , and ANS genes [ 38 ]. In Glycyrrhiza uralensis Fisch., two R2R3-MYB TFs, GlMYB4 and GlMYB88, positively regulate the biosynthesis of flavonoids in licorice cells [ 39 ]. Our previous study has identified an R2R3-type TmMYB3 that is involved in the phloem-specific accumulation of taxol by regulating the expression of the TBT and TS genes [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Role of female-predominant MYB39-bHLH13 complex in sexually dimorphic accumulation of taxol in Taxus media

Huang

et al. 2022

Horticulture Research

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Taxus trees are major natural sources for the extraction of taxol, a worldwide used anti-cancer agent. Taxus media is a dioecious woody tree with high taxol yield. However, the sexually dimorphic accumulation of taxoids in T. media is largely unknown. Our study revealed high accumulation of taxoids in the female T. media trees using a UPLC-MS/MS method. Thereafter, many differential metabolites and genes between female and male T. media trees were identified using metabolomic and transcriptomic analyses, respectively. Most of the taxol-related genes predominantly expressed in the female trees. A female-specific R2R3-MYB transcription factor, TmMYB39, was identified. Furthermore, BiFC and Y2H assays suggested the potential interaction between TmMYB39 and TmbHLH13. Several taxol biosynthesis-related promoter sequences were isolated and used for the screening of MYB recognition elements. EMSA analysis indicated that TmMYB39 could bind to the promoters of the GGPPS, T10OH, T13OH, and TBT genes. Interaction between TmMYB39 and TmbHLH13 trans-activated the expression of the GGPPS and T10OH genes. TmMYB39 might function in the transcriptional regulation of taxol biosynthesis through a “MYB-bHLH” module. Our results gave a potential explanation for the sexually dimorphic biosynthesis of taxol in T. media.

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Section: Discussionmentioning

confidence: 99%

Role of female-predominant MYB39-bHLH13 complex in sexually dimorphic accumulation of taxol in Taxus media

Huang

et al. 2022

Horticulture Research

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“…Flavonoids are a large category of secondary metabolites ubiquitous in medicinal plants. Flavonoids biosynthesize through the phenylpropanoid pathway from substrate phenylalanine initially which is then converted to cinnamic acid through PAL catalysis ( Ferrer et al, 2008 ; Li et al, 2020 ). We found that the expression of PAL genes was higher in ACS vs. AC.…”

Section: Discussionmentioning

confidence: 99%

Integrated transcriptome and metabolome analyses revealed regulatory mechanisms of flavonoid biosynthesis in Radix Ardisia

Liu

Pan

Yin

et al. 2022

PeerJ

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Background Radix Ardisia (Jab Bik Lik Jib) is a common Miao medicine and is widely distributed in the Guizhou region of southern China. The botanical origin of Radix Ardisia includes the dry root and rhizome of Ardisia Crenata Sims (ACS) or Ardisia Crispa (Thunb.) A.DC. (AC), which are closely related species morphologically. However, the secondary metabolites in their roots are different from one another, especially the flavonoids, and these differences have not been thoroughly explored at the molecular level. This project preliminarily identified regulatory molecular mechanisms in the biosynthetic pathways of the flavonoids between ACS and AC using a multi-omics association analysis. Methods In this study, we determined the total levels of saponin, flavonoid, and phenolic in Radix Ardisia from different origins. Integrated transcriptome and metabolome analyses were used to identify the differentially expressed genes (DEGs) and differentially expressed metabolites (DEM). We also performed conjoint analyses on DEGs and DEMs to ascertain the degree pathways, and explore the regulation of flavonoid biosynthesis. Results The total flavonoid and phenolic levels in ACS were significantly higher than in AC (P < 0.05). There were 17,685 DEGs between ACS vs. AC, 8,854 were upregulated and 8,831 were downregulated. Based on this, we continued to study the gene changes in the flavonoid biosynthesis pathway, and 100 DEGs involving flavonoid biosynthesis were differentially expressed in ACS and AC. We validated the accuracy of the RNA-seq data using qRT-PCR. Metabolomic analyses showed that 11 metabolites were involved in flavonoid biosynthesis including: Naringenin, Luteolin, Catechin, and Quercetin. A conjoint analysis of the genome-wide connection network revealed the differences in the types and levels of flavonoid compounds between ACS and AC. The correlation analysis showed that Naringenin, Luteolin, Catechin, and Quercetin were more likely to be key compounds in the flavonoid biosynthesis pathway also including 4CL, AOMT, CHS, CHI, DFR, F3’5’H, FLS, and LAR. Conclusions This study provides useful information for revealing the regulation of flavonoid biosynthesis and the regulatory relationship between metabolites and genes in the flavonoid biosynthesis pathway in Radix Ardisia from different origins.

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“…Jasmonic acid (JA) is a phytohormone ubiquitous in the plant kingdom [ 43 ] that regulates biological processes and secondary metabolite pathways in plants, including flavonoid biosynthesis [ 43 , 44 ]. Active jasmonate (JA-Ile) can induce the degradation of transcriptional repressors called the jasmonate ZIM-domain (JAZ) mediated by the SCF-COI1 complex, thus releasing the transcription factor MYC2 to play a regulatory role in gene expression [ 45 ].…”

Section: Hormones and The Gravitropic Response In Gymnospermsmentioning

confidence: 99%

A Talk between Flavonoids and Hormones to Reorient the Growth of Gymnosperms

Morales-Quintana

Ramos

2021

IJMS

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Plants reorient the growth of affected organs in response to the loss of gravity vector. In trees, this phenomenon has received special attention due to its importance for the forestry industry of conifer species. Sustainable management is a key factor in improving wood quality. It is of paramount importance to understand the molecular and genetic mechanisms underlying wood formation, together with the hormonal and environmental factors that affect wood formation and quality. Hormones are related to the modulation of vertical growth rectification. Many studies have resulted in a model that proposes differential growth in the stem due to unequal auxin and jasmonate allocation. Furthermore, many studies have suggested that in auxin distribution, flavonoids act as molecular controllers. It is well known that flavonoids affect auxin flux, and this is a new area of study to understand the intracellular concentrations and how these compounds can control the gravitropic response. In this review, we focused on different molecular aspects related to the hormonal role in flavonoid homeostasis and what has been done in conifer trees to identify molecular players that could take part during the gravitropic response and reduce low-quality wood formation.

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Two responses to MeJA induction of R2R3-MYB transcription factors regulate flavonoid accumulation in Glycyrrhiza uralensis Fisch

Cited by 24 publications

References 61 publications

Role of female-predominant MYB39-bHLH13 complex in sexually dimorphic accumulation of taxol in Taxus media

Role of female-predominant MYB39-bHLH13 complex in sexually dimorphic accumulation of taxol in Taxus media

Integrated transcriptome and metabolome analyses revealed regulatory mechanisms of flavonoid biosynthesis in Radix Ardisia

A Talk between Flavonoids and Hormones to Reorient the Growth of Gymnosperms

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