AB080. Annexin A5 regulates Leydig cell testosterone production via ERK1/2 pathway

Abstract

PubMed, and also report some research progress of acrosome formation in our laboratory. Results: Acrosome formation can be divided into four stages: Golgi-phase, cap-phase, acrosome-phase and maturation-phase. In the past 10 years, with gene targeting technology, more than ten genes were identified to be related acrosome formation in mice. Those genes include Casein kinase II α´ catalytic subunit (Csnk2a2), HIV-1 Revbinding protein (Hrb), Golgi-associated PDZ-and coiledcoil motif-containing protein (Gopc), Beta-glucosidase 2 (Gba2), Zona pellucida binding protein 1 (Zpbp1), protein interacting with C kinase 1 (Pick1), heat shock protein 90kDa beta member 1 (Hsp90β1), autophagy-related gene 7 (Atg7), sperm acrosome associated 1 (Spaca1), Dpy-19-like protein 2 (Dpy19l2) and stromal membraneassociated protein 2 (Smap2). Recently, we generated a Ccdc62 knockout mouse model with CRISPR-Cas9 system. A preliminary data showed that the male mice with Ccdc62 knockout were infertile, and 98% of sperm showed abnormal head with very lower motility, which suggested that Ccdc62 played a very important role in mouse acrosome formation. Globozoospermia is a rare type of teratozoospermia accounting for <0.1% of male infertility. It has reported that the mutation of SPATA16, PICK1 and DPY19L2 were related to clinical globozoospermia. Conclusions: The process of acrosome formation is regulated by multiple genes and its disorder will results in globozoospermia.

In Vitro Analysis of Deoxynivalenol Influence on Steroidogenesis in Prostate

“…To determine the effect of DON on steroidogenesis we evaluated the expression of genes related to steroidogenesis-CYP11A1, CYP17A1, CYP19A1, HSD3B2, HSD17B2 and StAR using RT-qPCR. We were also interested in ANX5A, due to the fact that annexin V is considered to regulate testosterone production [41]. We observed the highest relative expression of CYP11A1 in the PC-3 line compared to the rest of the investigated cell lines, and the differences were noticeable.…”