AaCOI1, Encoding a CORONATINE INSENSITIVE 1-Like Protein of Artemisia annua L., Is Involved in Development, Defense, and Anthocyanin Synthesis

Abstract

Artemisia annua is an important medicinal plant producing the majority of the antimalarial compound artemisinin. Jasmonates are potent inducers of artemisinin accumulation in Artemisisa annua plants. As the receptor of jasmonates, the F-box protein COI1 is critical to the JA signaling required for plant development, defense, and metabolic homeostasis. AaCOI1 from Artemisia annua, homologous to Arabidopsis AtCOI1, encodes a F-box protein located in the nuclei. Expressional profiles of the AaCOI1 in the root, stem, leaves, and inflorescence was investigated. The mRNA abundance of AaCOI1 was the highest in inflorescence, followed by in the leaves. Upon mechanical wounding or MeJA treatment, expression of AaCOI1 was upregulated after 6 h. When ectopically expressed, driven by the native promoter from Arabidopsis thaliana, AaCOI1 could partially complement the JA sensitivity and defense responses, but fully complemented the fertility, and the JA-induced anthocyanin accumulation in a coi1-16 loss-of-function mutant. Our study identifies the paralog of AtCOI1 in Artemisia annua, and revealed its implications in development, hormone signaling, defense, and metabolism. The results provide insight into JA perception in Artemisia annua, and pave the way for novel molecular breeding strategies in the canonical herbs to manipulate the anabolism of pharmaceutic compounds on the phytohormonal level.

Genome-wide identification and expression analysis of the coronatine-insensitive 1 (COI1) gene family in response to biotic and abiotic stresses in Saccharum

“…Currently, as a polygenic family, the COI1 gene has been identified and reported in many plants, such as A. thaliana [ 31 ], O. sativa [ 18 ], Artemisia annua [ 51 ], T. aestivum [ 27 ], and Hevea brasiliensis [ 50 ]. In this study, a total of 156 COI1 proteins were identified from 19 land plant genomes, including 21 SsCOI1s from S. spontaneum , three ShCOI1s from R570, and seven SbCOI1s from S. bicolor , but no COI1 was obtained from five algae plants.…”

Plant grafting relieves asymmetry of jasmonic acid response induced by wounding between scion and rootstock in tomato hypocotyl

“…In the damage treatment of Aquilaria sinensis , AsCOI1 showed a high increase in expression at 1 h, about 20-fold higher, and the highest expression level at 6 h, about 33-fold higher [ 32 ]. In addition, the expression of AaCOI1 in the leaves of Artemisia annua was significantly upregulated 6 h after wounding [ 33 ]. SlCOI1 is essential for JA induced gene expression in tomatoes, and the JA signaling pathway with SlCOI1 as the co-receptor plays a different role in controlling the expression levels of early and late response genes in wounding responses [ 34 ].…”