Plant grafting relieves asymmetry of jasmonic acid response induced by wounding between scion and rootstock in tomato hypocotyl

Abstract

Plant grafting is a sequential wound healing process. However, whether wounding induces a different jasmonic acid (JA) response within half a day (12 h) after grafting or non-grafting remains unclear. Using the tomato hypocotyl grafting method, we show that grafting alleviates the asymmetrical accumulation of JA and jasmonic acid isoleucine conjugate (JA-Ile) in scion and rootstock caused by wounding, and from 2 h after tomato micrografting, grafting obviously restored the level of JA-Ile in the scion and rootstock. Meanwhile, five JA-related genes, SlLOX11, SlAOS, SlCOI1, SlLAPA and SlJA2L, are detected and show significant changes in transcriptional expression patterns within 12 h of grafting, from asymmetrical to symmetrical, when the expression of 30 JA- and defense-related genes were analyzed. The results indicated that grafting alleviates the asymmetrical JA and defense response between scion and rootstock of the tomato hypocotyl within 12 h as induced by wounding. Moreover, we demonstrate that in the very early hours after grafting, JA-related genes may be involved in a molecular mechanism that changes asymmetrical expression as induced by wounding between scion and rootstock, thereby promoting wound healing and grafting success.

Effects of the Rhizosphere Fungus Cunninghamella bertholletiae on the Solanum lycopersicum Response to Diverse Abiotic Stresses

“…bertholletiae applications on numerous physiochemical parameters such as agronomic traits, phytohormone content, sugar content, and antioxidant activity in salinity, drought and heavy metal-stressed plants, (3) to elucidate the modulatory functions of C . bertholletiae in tomato plants under non-stress and stress conditions, and (4) to authenticate our physiochemical findings via the expression of patterns of different stress-responsive genes comprising SlACCase (Acetyl-coenzyme A carboxylase) [ 30 ], SlAOS (Allene oxide synthase) [ 31 ], SlICS (Isochorismate synthase ) [ 32 ], SlCDF3 (Cycling DNA binding with One Finger Factor) [ 33 ], SlGRAS6 (GAI; gibberellic-acid insensitive, RGA; repressor of GAI, SCR; scarecrow) [ 34 ], SlTAF1 (TATA-Box Binding Protein Associated Factor 1) [ 35 ], SlZH13 (Zinc finger-homeodomain protein) [ 36 ], SlRBOHD (Respiratory burst oxidase homolog protein D) [ 37 ], and SlRING1 (RING E3 ubiquitin ligase) [ 38 ].…”

Graft incompatibility between pepper and tomato can be attributed to genetic incompatibility between diverged immune systems

“…S11a-c, Table S12) All three of these hormones are known to play a role in defense processes, with SA serving a critical function in NLR-induced HR (Enyedi et al ., 1992; Lorenzo et al ., 2003; Koornneef & Pieterse, 2008). In addition, JA and ethylene are associated with graft junction formation (Wang et al ., 2020; Thomas et al ., 2022). At 7 DAG, SA, JA, and ethylene biosynthesis were upregulated in self and incompatible grafted samples relative to ungrafted controls, indicating that the 7 day response is dominated by general graft healing processes.…”