Untitled

Patolsky, Fernando; Lichtenstein, Amir; Kotler, Moshe; Willner, Itamar

doi:10.1002/1521-3757(20010618)113:12<2321::aid-ange2321>3.3.co;2-i

Cited by 13 publications

(18 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PCR [41,167,168], even on the electrode surface [113,115,116] (biological methods). The ultimate goal is that the label disturbs hybridisation specificity and efficiency as little as possible while maintaining the activity which provides good analytical response.…”

Section: Methods Based On Covalently Bound Markersmentioning

confidence: 99%

“…Willner's group has comprehensively shown that faradaic impedance spectroscopy is a very sensitive and versatile tool for detection of hybridisation and mutation detection but not very suitable for rapid biosensing [81,82,83,84,113,114,115,116,117]. A hybridisation event causes an increase in the negative charge on the interface.…”

Section: Impedancementioning

confidence: 99%

“…horseradish peroxidase (HRP) [81,82,83,84] or alkaline phosphatase [115,116], incorporated in the biotinylated reporter probe.…”

Section: Impedancementioning

confidence: 99%

“…These methods have been adapted to point mutation [113,115] and viral genome detection [116]. In these the polymerase (DNA) or transcriptase inverse (RNA) reaction proceeds in situ, i.e.…”

Section: Impedancementioning

confidence: 99%

See 3 more Smart Citations

Current strategies for electrochemical detection of DNA with solid electrodes

de-los-Santos-Álvarez

Lobo-Castañón

Miranda‐Ordieres

et al. 2004

Analytical and Bioanalytical Chemistry

115

View full text Add to dashboard Cite

A review of current strategies aimed at detecting nucleic acids (NA) using NA-modified solid electrodes reveals the versatility and potential of electrochemical detection in this field. What emerged at the beginning of 90s as a very promising detection system in DNA technology is now resulting in the first commercial devices. Many aspects of the experimental design, for example surface immobilisation and detection schemes, are outlined and evaluated. Although most approaches use hybridisation as the recognition reaction, those not based on hybridisation are also included. As is finally shown, great advances have been achieved, although further developments are required if electrochemical devices are to be suitable for routine measurement.

show abstract

Section: Methods Based On Covalently Bound Markersmentioning

confidence: 99%

Section: Impedancementioning

confidence: 99%

“…horseradish peroxidase (HRP) [81,82,83,84] or alkaline phosphatase [115,116], incorporated in the biotinylated reporter probe.…”

Section: Impedancementioning

confidence: 99%

“…These methods have been adapted to point mutation [113,115] and viral genome detection [116]. In these the polymerase (DNA) or transcriptase inverse (RNA) reaction proceeds in situ, i.e.…”

Section: Impedancementioning

confidence: 99%

See 2 more Smart Citations

Current strategies for electrochemical detection of DNA with solid electrodes

de-los-Santos-Álvarez

Lobo-Castañón

Miranda‐Ordieres

et al. 2004

Analytical and Bioanalytical Chemistry

115

View full text Add to dashboard Cite

show abstract

“…In the search for simpler techniques a number of electrochemical detection methods that dispense with labeling treatments are being studied, 2 and various signal amplification methods have been developed. These include types based on electroactive diffusion species, [3][4][5][6][7] intercalating or groove binding species, [8][9][10][11] ligation of nucleobases labeled with electroactive species, [12][13][14][15] and methods based on more than one mediator (intercalators, groove binders, or nucleobases) and diffusion markers. [16][17][18][19] The authors, in a previous report, achieved detection of target DNAs using a gold electrode modified with a probe peptide nucleic acid (PNA) possessing a ferrocene moiety as the signal part at one end and cysteine as an anchor part at the other end (Fc-PNA, Fig.…”

Section: Introductionmentioning

confidence: 99%

Signal Enhancement for Gene Detection Based on a Redox Reaction of [Fe(CN)6]4– Mediated by Ferrocene at the Terminal of a Peptide Nucleic Acid as a Probe with Hybridization-amenable Conformational Flexibility

Aoki

Tao

2008

ANAL. SCI.

View full text Add to dashboard Cite

Electrochemically enhanced DNA detection was demonstrated by utilizing the couple of a synthesized ferroceneterminated peptide nucleic acid (PNA) with a cysteine anchor and a sacrificial electron donor [Fe(CN)6] 4-. DNA detection sensors were prepared by modifying a gold electrode surface with a mixed monolayer of the probe PNA and 11-hydroxy-1-undecanethiol (11-HUT), protecting [Fe(CN)6] 4-from any unexpected redox reaction. Before hybridization, the terminal ferrocene moiety of the probe was subject to a redox reaction due to the flexible probe structure and, in the presence of [Fe(CN)6] 4-, the observed current was amplified based on regeneration of the ferrocene moiety. Hybridization decreased the redox current of the ferrocene. This occurred because hybridization rigidified the probe structure: the ferrocene moiety was then removed from the electrode surface, and the redox reaction of [Fe(CN)6] 4-was again prevented. The change in the anodic current before and after hybridization was enhanced 1.75-fold by using the electron donor [Fe(CN)6] 4-. Sequence-specific detection of the complementary target DNA was also demonstrated.

show abstract

Magnetically Amplified DNA Assays (MADA): Sensing of Viral DNA and Single‐Base Mismatches by Using Nucleic Acid Modified Magnetic Particles

et al. 2003

Self Cite

View full text Add to dashboard Cite

Die verbesserte Erkennung von viraler DNA und Einzelbasenmutationen wird durch die Einführung von Biotin‐Gruppen in DNA‐Replika auf magnetischen Teilchen erzielt. Die Biochemolumineszenz der DNA‐Erkennungs‐Ereignisse kann leichter elektrokatalytisch weitergegeben werden. Durch Rotieren der magnetischen Teilchen auf einer Elektrodenoberfläche werden die einzelnen Teilschritte zusätzlich verstärkt.

show abstract

Untitled

Cited by 13 publications

References 0 publications

Current strategies for electrochemical detection of DNA with solid electrodes

Current strategies for electrochemical detection of DNA with solid electrodes

Signal Enhancement for Gene Detection Based on a Redox Reaction of [Fe(CN)6]4– Mediated by Ferrocene at the Terminal of a Peptide Nucleic Acid as a Probe with Hybridization-amenable Conformational Flexibility

Magnetically Amplified DNA Assays (MADA): Sensing of Viral DNA and Single‐Base Mismatches by Using Nucleic Acid Modified Magnetic Particles

Contact Info

Product

Resources

About