A yeast protein analogous to Escherichia coli RecA protein whose cellular level is enhanced after UV irradiation

Angulo, Jaime F.; Schwencke, Jaime; Moreau, P.; Moustacchi, E.; Devoret, Raymond

doi:10.1007/bf00397980

Cited by 34 publications

(8 citation statements)

References 31 publications

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“…Given the importance of recombination and DNA repair in virtually all living systems, it is possible that an essential and versatile protein such as RecA may have been highly conserved throughout evolution. The detection of RecA-like activities in eukaryotic organisms such as Ustilago (37) and Saccharomyces (38)(39)(40) raises interesting questions as to the potential functional and structural similarities between these proteins and their counterparts in prokaryotic organisms.…”

Section: Discussionmentioning

confidence: 99%

Induction of the Bacillus subtilis SOS-like response by Escherichia coli RecA protein.

Love¹,

Yasbin²

1986

Proc. Natl. Acad. Sci. U.S.A.

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A plasmid that expresses the Escherichia coli RecA protein partially restored DNA repair and recombination capability and induction of the SOS-like (SOB) response in a recE4 mutant of Bacillus subtilis. In the presence of DNAdamaging agents, the E. coli RecA protein induced din operon expression, Weigle-reactivation activity, and synthesis of a B.subtilis recombination protein (Recbs) analogous to RecA but was unable to stimulate prophage induction. In addition, the RecA protein was capable of inducing the SOB response in competent recE4 strains of B. sublitis, independent of exposure to DNA-damaging agents. The results suggest that (i) the SOS response of E. coli and the SOB response of B. subtilis are strikingly similar from both a phenotypic and a regulatory standpoint and that RecA and LexA protein analogs exist in B. subtilis, (it) the Recbs protein is capable of regulating its own production, and (iii) SOS-inducing (RecA-activating) signals are generated in B. subtilis following either DNA damage or the development of physiological competence.

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Section: Discussionmentioning

confidence: 99%

Induction of the Bacillus subtilis SOS-like response by Escherichia coli RecA protein.

Love¹,

Yasbin²

1986

Proc. Natl. Acad. Sci. U.S.A.

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“…Several DNA-damage-inducible genes have been identified in S. cerevisiae, and these include the DNA ligase gene CDC9 (36), and several genes of unknown function: din genes (46), ddr genes (26), and the RecAsc gene (1 (35), RADIO (42), and the CDC9 genes (2) reveals a short common sequence in the RAD2, RAD7, and RADIO genes (Fig. 10).…”

Section: Resultsmentioning

confidence: 99%

Nucleotide sequence, transcript mapping, and regulation of the RAD2 gene of Saccharomyces cerevisiae

Madura¹,

Prakash²

1986

J Bacteriol

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We determined the nucleotide sequence, mapped the 5' and 3' mRNA termini, and examined the regulation of the RAD2 The RAD2 gene of Saccharomyces cerevisiae is one of 10 genes, RADI, RAD2, RAD3, RAI4, RAD7, RADIO, RAD14,-RAD16, RAD23, and MMS19, involved in excision repair of DNA containing pyrimidine dimers or cross-links (27,28,44,55). Mutants in the RADI, RAD2, RAD3, RAD4, RADIO, and MMSJ9 genes are highly defective in incision activity (27,44,55), while mutants in the other four genes show various degrees of incision defects (27,28,55). To study the structure, regulation, and function of these genes, we and others have cloned and characterized the RADI (15, 56), RAD2 (13, 33), RAD3 (14, 31, 32, 41), RAD7 (35), and RADIO (37, 42, 54) genes. We had previously located the and RADIO genes (15,37,42). In contrast, disruptions or deletions of the RAD3 gene are recessive lethal mutations (14,32,41). In this paper we report the complete nucleotide sequence of the RAD2 gene, map its 5' and 3' mRNA termini, and show that steady-state levels of RAIJ2 mRNA increase significantly after UV irradiation of yeast cells. MATERIALS AND METHODSYeast and bacterial strains. S. cerevisiae 7799-4B MATa his4-17 ura3-52 RAD+ was used for transcript analyses, and strain DBY746 MATa his3-Al leu2-3 leu2-112 trpl-289 ura3-52 RAD+ was used for RAD2-lacZ fusion analyses.

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“…Low basal activity was observed by direct assay ( Table 2). As some DNA repair genes from yeasts are known to be inducible by DNA-damaging agents such as UV (1,9,27,28,41,46,48) or 4-nitroquinolone-N-oxide (27, 48), we monitored p-galactosidase activity following treatment with these agents. In addition, the response to heat shock was examined.…”

Section: Resultsmentioning

confidence: 99%

The REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis

1989

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The REV) gene of Saccharomyces cerevisiae is required for nora induction of mutatio by pysical ad chemical agents. We (23,24).The REV) gene product is thought to constitute part of a DNA repair process that is "error prone" or "'mutagenic" in its function (14,20,25). Just as SOS processing by umuDC is distinguished from other Rec functions and from uvrABCcontrolled excision repair in Escherichia coli (56), mutagenic repair is distinct from the other major repair pathways in yeasts (14,20,25 In this paper we describe the isolation of the REV) gene and the mutant allele rev)-) and report their nucleotide and deduced amino acid sequences. Deletion analysis was used to determine the functional limits of the REVI coding sequence and its 5'-and 3'-flanking sequences. A preliminary characterization of the response of REVl::lacZ fusions to heat shock and DNA-damaging agents is presented. Similarity analysis suggests that a segment of the REVI protein is homologous to UMUC protein. MATERIALS AND METHODSMedia. LB medium (29) (with antibiotic supplements as appropriate) was used to propagate E. coli strains and plasmid-bearing derivatives. YEPD and synthetic complete (SC) yeast media have been described before (24). SC-URA is SC without uracil, SC-LYS is SC without lysine, and SC-LEU is SC without leucine.Yeast strains. S. cerevisiae S288C a gal2 mel mal SUC2 CUP) was used as the source of wild-type DNA for library construction. Strains XL68-3D a rev)-) lys)-) leu2-3 his4-38 trpl-289 ura3-52, XL69-5A a REVI lys)-) leu2-3 trpl-289 his3M, and XL7O-1B a RPV) lysl-l leu2-3 trp)-289 ura3-52 were constructed in this laboratory, using standard techniques for mating, sporulation, and tetrad analysis (52).Bacterial strains. E. coli BNN45 (F metB thi hsdR lacY supE44 supF), derived from ED8654 (31), was used routinely as a cloning host. E. coli DH1 recAl (F-Alac-pro, endA) gyrA96 thi-) hsdR) 7 supE44 relAI) (13) was used as a cosmid host. E. coli JM107 (Alac-pro endAl gyrA96 thi-) hsdR)7 supE44 relA) (F' traD36 proAB+ lacIZAM)5]) (57) was used as the host for M13 vectors. E. coli GM215 (F-dam-3 endAl rna-) thi-) supE44) (30) Was used to produce Damplasmid DNA for BclI subcloning.Plasmids, enzymes, and biochemicals. Plasmids YCp5O, YIp5, YEp13, and pHC79 have been described previously (6,15, 19,50

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A yeast protein analogous to Escherichia coli RecA protein whose cellular level is enhanced after UV irradiation

Cited by 34 publications

References 31 publications

Induction of the Bacillus subtilis SOS-like response by Escherichia coli RecA protein.

Induction of the Bacillus subtilis SOS-like response by Escherichia coli RecA protein.

Nucleotide sequence, transcript mapping, and regulation of the RAD2 gene of Saccharomyces cerevisiae

The REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis

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