A wheat salinity-induced WRKY transcription factor TaWRKY93 confers multiple abiotic stress tolerance in Arabidopsis thaliana

Qin, Yuxiang; Tian, Yanchen; Liu, Xiuzhi

doi:10.1016/j.bbrc.2015.06.128

Cited by 127 publications

(69 citation statements)

References 35 publications

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“…Weights of the samples were recorded at regular intervals. Determination of electrolyte leakage was performed as described by Qin et al (2015). Free proline concentrations in leaf extracts from drought-stressed wild type and transgenic Arabidopsis were determined as described by Bates et al (1973).…”

Section: Measurement Of Leaf Water Loss Electrolyte Leakage and Prolmentioning

confidence: 99%

OsMSR15 encoding a rice C2H2-type zinc finger protein confers enhanced drought tolerance in transgenic Arabidopsis

Zhang

et al. 2016

J. Plant Biol.

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Zinc nger proteins (ZFPs) play important roles in plant responses to biotic and abiotic stresses. Through microarray analysis, an Oryza sativa L. multi-stress-responsive gene, OsMSR15, was identied and subsequently cloned from rice Pei'ai 64S (Oryza sativa L.). Expression of OsMSR15 was strongly up-regulated by cold, drought and heat stresses in different tissues at different developmental stages of rice. OsMSR15 contains two C2H2-type zinc nger motifs, a nuclear localization signal (B box), a Leu-rich domain (Lbox) and a conserved EAR-motif close to its C-terminus. The OsMSR15-GFP fusion protein was localized to the nucleus. Yeast-one hybrid assay showed that OsMSR15 possesses transcriptional activation ability. Expression of OsMSR15 in Arabidopsis conferred drought tolerance, and transgenic plants showed hypersensitivity to exogenous ABA during the seed germination and post-germination stages. Transgenic plants also showed higher levels of free proline, less electrolyte leakage and increased expressions of a number of stress-responsive genes, including LEA3, RD29A, DREB1A and P5CS1 under drought stress. The obtained results indicate that OsMSR15 is an important regulator involved in plant response to drought stress.

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Section: Measurement Of Leaf Water Loss Electrolyte Leakage and Prolmentioning

confidence: 99%

OsMSR15 encoding a rice C2H2-type zinc finger protein confers enhanced drought tolerance in transgenic Arabidopsis

Zhang

et al. 2016

J. Plant Biol.

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“…Similarly, in rice, overexpression of OsWRKY11, OsWRKY45, and OsWRK72 results in enhanced drought tolerance (Qiu and Yu, 2009; Wu et al, 2009; Song et al, 2010; Ding et al, 2014). In other crops, HvWRKY38, TaWRKY1, TaWRKY33, TaWRKY44, and TaWRKY93 are also involved in the drought response (Marè et al, 2004; Qin et al, 2015; Wang X. et al, 2015). However, compared the grass family, the action of WRKY proteins in legumes is limited.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Investigation of WRKY Transcription Factors Involved in Terminal Drought Stress Response in Common Bean

Chen

Wang

et al. 2017

Front. Plant Sci.

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WRKY transcription factor plays a key role in drought stress. However, the characteristics of the WRKY gene family in the common bean (Phaseolus vulgaris L.) are unknown. In this study, we identified 88 complete WRKY proteins from the draft genome sequence of the “G19833” common bean. The predicted genes were non-randomly distributed in all chromosomes. Basic information, amino acid motifs, phylogenetic tree and the expression patterns of PvWRKY genes were analyzed, and the proteins were classified into groups 1, 2, and 3. Group 2 was further divided into five subgroups: 2a, 2b, 2c, 2d, and 2e. Finally, we detected 19 WRKY genes that were responsive to drought stress using qRT-PCR; 11 were down-regulated, and 8 were up-regulated under drought stress. This study comprehensively examines WRKY proteins in the common bean, a model food legume, and it provides a foundation for the functional characterization of the WRKY family and opportunities for understanding the mechanisms of drought stress tolerance in this plant.

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“…ABA helps mitigate drought through closure of guard cells of the stomata (Tuteja, 2007; Yoshida et al, 2015), simultaneously regulating the expression of several drought associated and cold stress responsive genes ( ABI genes, MYB2, RAB18, RD29A, ABF4, AOX1, DREB2 , etc. ; Rushton et al, 2012; Qin et al, 2015). The occurrence of ABRE and W-box elements upstream to the coding regions of specialized metabolism genes further determines the plant response to ABA under stress (Fujita et al, 2005; Gómes-Porras et al, 2007; Yoshida et al, 2015).…”

Section: Stress Conditions Regulating Primary and Specialized Metabolmentioning

confidence: 99%

“…It was also noted that WRKYs have a significant role in upregulating several other stress-responsive genes (like ABF3, ABIs, DREB2A, RDs , etc. ; Qin et al, 2015). The synergistic binding of WRKY to W-box ; ABF to ABRE ; MYB TFs to MYB recognition elements; CBFs to LTRE and GBFs to GATA was found to upregulate SamDC gene in O. sativa (Basu et al, 2014).…”

Section: Stress Conditions Regulating Primary and Specialized Metabolmentioning

confidence: 99%

“…Additionally, reports suggest that in O. sativa , the presence of W-box elements upstream to the polyamine synthesis gene ( SamDC ) plays a key role in its upregulation (Basu et al, 2014). Furthermore, Ta WRKY93 (WRKY protein from wheat) was found to enhance the levels of Pyrroline-5-carboxylate synthase (P5CS; Qin et al, 2015) involved in proline biosynthesis (Proline is known to be directly involved in drought mitigation as osmoticum; Chamoli and Verma, 2014). It was also noted that WRKYs have a significant role in upregulating several other stress-responsive genes (like ABF3, ABIs, DREB2A, RDs , etc.…”

Section: Stress Conditions Regulating Primary and Specialized Metabolmentioning

confidence: 99%

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Stress-Mediated cis-Element Transcription Factor Interactions Interconnecting Primary and Specialized Metabolism in planta

2016

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Plant specialized metabolites are being used worldwide as therapeutic agents against several diseases. Since the precursors for specialized metabolites come through primary metabolism, extensive investigations have been carried out to understand the detailed connection between primary and specialized metabolism at various levels. Stress regulates the expression of primary and specialized metabolism genes at the transcriptional level via transcription factors binding to specific cis-elements. The presence of varied cis-element signatures upstream to different stress-responsive genes and their transcription factor binding patterns provide a prospective molecular link among diverse metabolic pathways. The pattern of occurrence of these cis-elements (overrepresentation/common) decipher the mechanism of stress-responsive upregulation of downstream genes, simultaneously forming a molecular bridge between primary and specialized metabolisms. Though many studies have been conducted on the transcriptional regulation of stress-mediated primary or specialized metabolism genes, but not much data is available with regard to cis-element signatures and transcription factors that simultaneously modulate both pathway genes. Hence, our major focus would be to present a comprehensive analysis of the stress-mediated interconnection between primary and specialized metabolism genes via the interaction between different transcription factors and their corresponding cis-elements. In future, this study could be further utilized for the overexpression of the specific transcription factors that upregulate both primary and specialized metabolism, thereby simultaneously improving the yield and therapeutic content of plants.

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A wheat salinity-induced WRKY transcription factor TaWRKY93 confers multiple abiotic stress tolerance in Arabidopsis thaliana

Cited by 127 publications

References 35 publications

OsMSR15 encoding a rice C2H2-type zinc finger protein confers enhanced drought tolerance in transgenic Arabidopsis

OsMSR15 encoding a rice C2H2-type zinc finger protein confers enhanced drought tolerance in transgenic Arabidopsis

Genome-Wide Investigation of WRKY Transcription Factors Involved in Terminal Drought Stress Response in Common Bean

Stress-Mediated cis-Element Transcription Factor Interactions Interconnecting Primary and Specialized Metabolism in planta

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