Genome-Wide Investigation of WRKY Transcription Factors Involved in Terminal Drought Stress Response in Common Bean

Abstract

WRKY transcription factor plays a key role in drought stress. However, the characteristics of the WRKY gene family in the common bean (Phaseolus vulgaris L.) are unknown. In this study, we identified 88 complete WRKY proteins from the draft genome sequence of the “G19833” common bean. The predicted genes were non-randomly distributed in all chromosomes. Basic information, amino acid motifs, phylogenetic tree and the expression patterns of PvWRKY genes were analyzed, and the proteins were classified into groups 1, 2, and 3. Group 2 was further divided into five subgroups: 2a, 2b, 2c, 2d, and 2e. Finally, we detected 19 WRKY genes that were responsive to drought stress using qRT-PCR; 11 were down-regulated, and 8 were up-regulated under drought stress. This study comprehensively examines WRKY proteins in the common bean, a model food legume, and it provides a foundation for the functional characterization of the WRKY family and opportunities for understanding the mechanisms of drought stress tolerance in this plant.

Genome-wide identification and functional characterization of Camelina sativa WRKY gene family in response to abiotic stress

“…The higher proportion of group II members was also detected in other plant WRKY families such as 58% in Arabidopsis, 64% in Caragana intermedia [48] and 66% in Manihot esculenta [49]. For each group, CsWRKY members exhibited the similar structure, but members in different group showed speci c feature, which is in agreement with reports in other plants such as chickpea [50] and common bean [47].…”

“…However, eleven variants including WRKYGXK, WRKYGKK, and WRKYGEK for this domain were detected in a number of CsWRKY proteins in group IIc. In supporting of this, several variants of WRKY domain were also found in in other plant species [13,40,51,52], suggesting that these variants might give WRKYs multiple biological functions [47] although it is worthy of future research. In addition, we also identi ed several CsWRKY proteins as the chimeric proteins containing both R-protein conserved domain and WRKY domain.…”

“…This indicates that a large-scale expansion of this family happened in C. sativa genome, which might be results of gene duplication (see discussion below). Subsequently, these CsWRKY proteins were characterized according to the speci c conserved sequences of WRKYs and number of their WRKY zinc-nger motifs [35], following the principle used Arabidopsis [35] and Common bean [47]. Based on phylogenetic analysis of CsWRKYs and AtWRKYs, 242 CsWRKYs were divided into three main groups: Group I, Group II (subgroup IIa, IIb, IIc, IId, and IIe) and Group III, with group II containing 149 members which accounts for the largest proportion (62%) among all the groups.…”

Identification of WRKY Gene Family from Dimocarpus longan and Its Expression Analysis during Flower Induction and Abiotic Stress Responses

“…Morus005757 shows significant up-regulation in response to dehydration stress, salinity stress, and SA and ABA (Abscisic acid) treatments [27]. Similar results were also found in wheat, common bean [28], grape [29], pineapple [30], soybean [31], moso bamboo [32], Caragana intermedia [33], peanut [34], and broomcorn millet [35]. These observations suggest that studying the WRKY gene families may provide valuable insights into the mechanism underlying abiotic stress tolerance in plants.…”

Comparative Physiological, Biochemical and Transcript Response to Drought in Sorghum Genotypes

“…Also, NAC1, MYB2 and WRKY1 plays a vital role in the transcriptional regulation and signal transduction in response to drought stress [46][47][48]. Significantly and differentially increase in the expression level of these genes was observed under 14 d drought treatment (Fig.…”