A Voltammetric Study on the Interaction of Novobiocin With Cysteine

Bçer, Ender; Qetnkaya, Pakze

doi:10.4067/s0717-97072009000100011

Cited by 4 publications

(1 citation statement)

References 29 publications

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“…Also, CYS has been identified as a valuable biomarker [18]. There are therefore numerous research studies focused on the interactions of CYS with folates, catechol, quinoids, benzoquinones, and some drugs [18][19][20][21][22][23][24][25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Combined computational and experimental studies on cysteine-sulfadiazine adduct formation

2020

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The electrochemical characterization of sulfadiazine-cysteine (SD-CYS) adduct formation was performed in phosphate buffer (pH 7) on the basis of voltammetric current and peak potential measurements. Due to the association of cysteine with sulfadiazine, the reduction peak currents of mercuric and mercurous cysteine thiolates decreased and their peak potentials simultaneously shifted to less negative potentials. By using the current changes of mercurous cysteine thiolate, it was determined that cysteine and sulfadiazine are associated with a 1:1 stoichiometry with a conditional association constant of 1.99 ×10 4 M −1. In addition to experimental studies, a computational approach was carried out to study the geometrical parameters, electron densities, and UV-Vis absorption spectra of sulfadiazine and SD-CYS adduct in water. Calculated (B3LYP/6-311 ++ G(d,p) level) and experimental UV-Vis absorption spectra of the compounds were found to be in good agreement in water. The computational study suggests that cysteine bound to the C(5) on the pyrimidine ring via SH-group nucleophilic attack. Computational results reveal that sulfadiazine and its derivatives effectively bind cysteine and may lead to new molecules/drugs to target cysteine.

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Section: Introductionmentioning

confidence: 99%

Combined computational and experimental studies on cysteine-sulfadiazine adduct formation

2020

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Reduction of Ru^VI≡N to Ru^III—NH₃ by Cysteine in Aqueous Solution

et al. 2018

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The reduction of metal nitride to ammonia is a key step in biological and chemical nitrogen fixation. We report herein the facile reduction of a ruthenium(VI) nitrido complex [(L)Ru(N)(OH)] (1, L = N, N'-bis(salicylidene)- o-cyclohexyldiamine dianion) to [(L)Ru(NH)(OH)] by l-cysteine (Cys), an ubiquitous biological reductant, in aqueous solution. At pH 1.0-5.3, the reaction has the following stoichiometry: [(L)Ru(N)(OH)] + 3HSCHCH(NH)CO → [(L)Ru(NH)(OH)] + 1.5(SCHCH(NH)CO). Kinetic studies show that at pH 1 the reaction consists of two phases, while at pH 5 there are three distinct phases. For all phases the rate law is rate = k[1][Cys]. Studies on the effects of acidity indicate that both HSCHCH(NH)CO and SCHCH(NH)CO are kinetically active species. At pH 1, the reaction is proposed to go through [(L)Ru(NHSCHCHNHCOH)(OH)] (2a), [(L)Ru(NHSCHCHNHCOH)(OH)] (3), and [(L)Ru(NH)(OH)] (4) intermediates. On the other hand, at pH around 5, the proposed intermediates are [(L)Ru(NHSCHCHNHCO)(OH)] (2b) and [(L)Ru(NH)(OH)] (4). The intermediate ruthenium(IV) sulfilamido species, [(L)Ru(NHSCHCHNHCOH)(OH)] (2a) and the final ruthenium(III) ammine species, [(L)Ru(NH)(MeOH)] (5) (where HO was replaced by MeOH) have been isolated and characterized by various spectroscopic methods.

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Density Functional Theory Study of Cu₆ Nanoclusters as a Phenylalanine Detector

Bahota,

Singh,

Yadav

et al. 2023

ACS Omega

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Research on amino acids is an attractive area because of their application in metabolism, cancer treatment, growth, and repair of body tissue, and RNA and DNA syntheses. Twenty amino acids are primarily responsible for protein synthesis. In our study, we used a Cu 6 nanocluster as an amino acid detector. For the investigation, we adsorbed amino acids on the Cu 6 nanocluster and studied their UV−visible spectra. It is observed that all of the Cu 6 −amino acid complexes have peaks at near 380 nm wavelength except the Cu−phenylalanine complex, where two UV−visible peaks are found at wavelengths 351 nm (excitation energy 3.49 eV) and 403 nm (excitation energy 3.02 eV), respectively, which originated from the HOMO − 2 to LUMO (28%) and HOMO − 1 to LUMO (38%) transitions. Due to this unique transition, the Cu 6 nanocluster can be used for the detection of the phenylalanine amino acid out of the 20 amino acids.

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A Voltammetric Study on the Interaction of Novobiocin With Cysteine

Cited by 4 publications

References 29 publications

Combined computational and experimental studies on cysteine-sulfadiazine adduct formation

Combined computational and experimental studies on cysteine-sulfadiazine adduct formation

Reduction of Ru^VI≡N to Ru^III—NH₃ by Cysteine in Aqueous Solution

Density Functional Theory Study of Cu₆ Nanoclusters as a Phenylalanine Detector

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A Voltammetric Study on the Interaction of Novobiocin With Cysteine

Cited by 4 publications

References 29 publications

Combined computational and experimental studies on cysteine-sulfadiazine adduct formation

Combined computational and experimental studies on cysteine-sulfadiazine adduct formation

Reduction of RuVI≡N to RuIII—NH3 by Cysteine in Aqueous Solution

Density Functional Theory Study of Cu6 Nanoclusters as a Phenylalanine Detector

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Reduction of Ru^VI≡N to Ru^III—NH₃ by Cysteine in Aqueous Solution

Density Functional Theory Study of Cu₆ Nanoclusters as a Phenylalanine Detector