A Virus Isolated from Sugar Maple

Lana, Allan Femi; Thomas, O. T.; Peterson, J. F.

doi:10.1111/j.1439-0434.1980.tb03689.x

Cited by 6 publications

(5 citation statements)

References 2 publications

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“…300 nm-long and genomic material from tobamoviruses were observed by EM in symptomatic leaves. Rod-shaped virus particles were also detected in young A. saccharum seedlings with chlorotic spots and mottle symptoms ( Lana et al, 1980 ), which were attributed to tobacco mosaic virus based on their serological and biological properties. Isometric particles of 26–30 nm diameter were detected in maple trees from Turkish urban areas ( A. negundo, A. pseudoplatanus, Acer campestre ) exhibiting mottling, mosaic, leaf deformation and lateral shoot formation ( Erdiller, 1986 ).…”

Section: Introductionmentioning

confidence: 99%

Next-Generation Sequencing Reveals a Novel Emaravirus in Diseased Maple Trees From a German Urban Forest

et al. 2021

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While the focus of plant virology has been mainly on horticultural and field crops as well as fruit trees, little information is available on viruses that infect forest trees. Utilization of next-generation sequencing (NGS) methodologies has revealed a significant number of viruses in forest trees and urban parks. In the present study, the full-length genome of a novel Emaravirus has been identified and characterized from sycamore maple (Acer pseudoplatanus) – a tree species of significant importance in urban and forest areas – showing leaf mottle symptoms. RNA-Seq was performed on the Illumina HiSeq2500 system using RNA preparations from a symptomatic and a symptomless maple tree. The sequence assembly and analysis revealed the presence of six genomic RNA segments in the symptomatic sample (RNA1: 7,074 nt-long encoding the viral replicase; RNA2: 2,289 nt-long encoding the glycoprotein precursor; RNA3: 1,525 nt-long encoding the nucleocapsid protein; RNA4: 1,533 nt-long encoding the putative movement protein; RNA5: 1,825 nt-long encoding a hypothetical protein P5; RNA6: 1,179 nt-long encoding a hypothetical protein P6). Two independent NGS sequencing runs from the same symptomatic maple tree detected the same genome segments. For one of these sequencing runs the cDNA library was prepared using a primer targeting the conserved genome terminal region, known to be shared between emaraviruses genome segments. We suggest, therefore, that the six identified genome segments represent the complete genome of a novel emaravirus from maple, which we tentatively name maple mottle-associated virus (MaMaV). Phylogenetic and sequence homology analyses place this virus on the distinct “subgroup a” clade within the Emaravirus genus along with – among others – rose rosette virus, Actinidia emaravirus 2, and fig mosaic virus. Validation RT-PCR assays performed on symptomatic and non-symptomatic trees suggest that MaMaV may be the symptom-inducing virus in the diseased trees. To our knowledge, this is the first time an Emaravirus is described from maple and is fully genetically characterized. With the discovery of MaMaV, the genus Emaravirus comprising negative-sense single-stranded viruses with very divergent genomes – that were until recently overlooked – has substantially increased counting 22 established and putative members.

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Section: Introductionmentioning

confidence: 99%

Next-Generation Sequencing Reveals a Novel Emaravirus in Diseased Maple Trees From a German Urban Forest

et al. 2021

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“…Tobamoviruses are present in forest soils in New York State, from which they may infect forest trees. Both TMV and ToMV have been detected in forest, shade, and ornamental tree species (9,11,25,28,35), which could then serve as reservoir hosts to maintain these viruses in forest ecosystems. Subsequent, abiotic soil transmission to trees may provide the mechanism for the localized spread of these viruses and their persistence in forest ecosystems.…”

Section: Discussionmentioning

confidence: 99%

Detection of Infectious Tobamoviruses in Forest Soils

Fillhart

Bachand

Castello

1998

Appl Environ Microbiol

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Our objectives were to evaluate elution and bait plant methods to detect infectious tobamoviruses in forest soils in New York State. Soils were collected from two forest sites: Whiteface Mountain (WF) and Heiberg Forest (HF). The effectiveness of four buffers to elute tomato mosaic tobamovirus (ToMV) from organic and mineral fractions of WF soil amended with ToMV was tested, and virus content was assessed by enzyme-linked immunosorbent assay (ELISA). The effectiveness ofChenopodium quinoa (Willd.) bait plants to detect the virus also was tested. Both methods then were utilized to detect tobamoviruses in 11 WF and 2 HF soil samples. A phosphate buffer (100 mM, pH 7.0) eluted more ToMV from soil than the other buffers tested. Mineral soil bound more virus than organic soil. Virus recoveries from virus-amended organic and mineral soils were 3 and 10%, respectively, and the detection sensitivity was 10 to 20 ng/g of soil. Roots of bait plants grown in all virus-amended soils tested positive by ELISA, and virus concentrations averaged 10 ng/g. Both ToMV and tobacco mosaic tobamovirus (TMV) were transmitted to C. quinoa by elution from one of two HF soil samples but not from the WF soil samples. A tobamovirus was detected by bait planting in 12 of 73 (16%) root extracts representing 5 of 13 soil samples (38%). Tobamovirus-like particles were seen by transmission electron microscopy in 6 of 12 infected root extracts. Tobamoviruses occur in forest soils in New York State. Abiotic soil transmission to trees may permit localized spread and persistence of these viruses in forest ecosystems.

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“…У сучасній літературі рідко трапляються результати з виявлення вірусів у лісових екосистемах порівняно з вивченням хвороб вірусного походження в агроценозах. Разом з тим останнім часом віруси були ідентифіковані на листопадних плодових рослинах і деяких широколистяних і хвойних деревах [4][5][6][7].…”

Section: результати та їх обговоренняunclassified

“…Застосування пестицидів на орних землях нині залишається одним з найбільш згубних антропогенних чинників для польової фауни всього Європейського континенту [1][2][3][4][5][6][7]. Так, вчені наголошують, що негативна дія пестицидів на тварин полягає не тільки у безпосередньо спричиненій загибелі внаслідок гострого отруєння.…”

Section: екологічне навантаження на мисливські ресурси агроландшафтівunclassified

“…Слід зауважити, що такі чинники, як радіаційне навантаження [1,2], різка зміна клімату, порушення гідрологічного режиму, безгосподарна діяльність людини, індуку-ють мінливість і появу нових патогенів, що є небезпечними для деревних і трав'яних рослин, грибів і біоти загалом. До таких патогенів відносяться і вірусні хвороби [3][4][5][6].…”

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Screening and biological properties of ТМV isolates (Tobamovirus) on plants of eastern plane (Platanus orientalis L.) and Norway maple (Acer platanoides L.)

Orlovskyi¹,

Moroz²,

Boiko³

2016

agro

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Researches for the viral infection in woody plants of Ukraine forest biocenosis were first studied. Sampling was conducted in forests, parks and gardens in the Ukrainian Polissya Area (Zhytomyr, Kyiv region). Visual studies, electron microscopy, ELISA, method of internally cell inclusions, indicator plants, computer support (microscope-object-monitor) are used in work. TMV isolate mainly causes necrosis in Platanus orientalis and induces a kind of mosaic symptoms that turns into chlorosis during July — August in Acer platanoides. Other symptoms are observed such as leaves twisting and dying off, necrosis, wood damage and premature defoliation. It is found that identified TMV isolate has specific serological similarity with viruses of taxonomic groups that affect tomato, tobacco, sunflower seeds, and medical plants such as plantain and hops

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A Virus Isolated from Sugar Maple

Cited by 6 publications

References 2 publications

Next-Generation Sequencing Reveals a Novel Emaravirus in Diseased Maple Trees From a German Urban Forest

Next-Generation Sequencing Reveals a Novel Emaravirus in Diseased Maple Trees From a German Urban Forest

Detection of Infectious Tobamoviruses in Forest Soils

Screening and biological properties of ТМV isolates (Tobamovirus) on plants of eastern plane (Platanus orientalis L.) and Norway maple (Acer platanoides L.)

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