A Viral Epizootic in Cultured Populations of Juvenile Goldfish Due to a Putative Herpesvirus Etiology

Groff, J. M.; LaPatra, Scott E.; Munn, Robert J.; Zinkl, Joseph G.

doi:10.1177/104063879801000415

Cited by 78 publications

(96 citation statements)

References 10 publications

(14 reference statements)

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“…Mortality associated with HVHN can approach 100% (Jung & Miyazaki 1995, Groff et al 1998, Chang et al 1999, Stephens et al 2004. Herpesviral hematopoietic necrosis is caused by Cyprinid herpesvirus 2 (CyHV-2), a member of the cyprinid herpesvirus group that includes carp pox (CyHV-1) and koi herpesvirus (CyHV-3) (Waltzek et al 2005).…”

Section: Herpesviral Hematopoietic Necrosis (Hvhn) Is a Disease Of Gomentioning

confidence: 99%

Detection of the herpesviral hematopoietic necrosis disease agent (Cyprinid herpesvirus 2) in moribund and healthy goldfish: validation of a quantitative PCR diagnostic method

Goodwin

Merry²,

Sadler³

2006

Dis. Aquat. Org.

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Cyprinid herpesvirus 2 (CyHV-2) is a pathogen of goldfish Carassius auratus auratus L. that causes herpesviral hematopoietic necrosis (HVHN) disease. The disease is associated with necrosis of hematopoietic tissues and anemia with high mortality. We have developed a real time 5'-nuclease PCR method (Taqman) that quantitatively detects CyHV-2 with a linear response over 8 logs of target concentration. The coefficient of variability on replicate samples tested on different days was 13% and the calculated sensitivity approached 1 target molecule per reaction. The assay does not cross-react with other similar fish herpesviruses, including CyHV-1 (carp pox) and CyHV-3 (koi herpesvirus), but reliably detects known CyHV-2 positive fish. The assay detects CyHV-2 not just in clinical cases of HVHN but also in apparently healthy 1 yr old goldfish fingerlings and even in 3 to 5 yr old broodfish.

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Section: Herpesviral Hematopoietic Necrosis (Hvhn) Is a Disease Of Gomentioning

confidence: 99%

Detection of the herpesviral hematopoietic necrosis disease agent (Cyprinid herpesvirus 2) in moribund and healthy goldfish: validation of a quantitative PCR diagnostic method

Goodwin

Merry²,

Sadler³

2006

Dis. Aquat. Org.

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“…This is in contrast to previous reports, where intranuclear inclusion bodies with marginated chromatin were regularly found. 2,5,[8][9][10]12,14 However, the lack of inclusions in our study could be a result of the early stage of disease, as most investigated animals were subclinically infected. This is supported by the fact that despite the lack of inclusion bodies, CyHV-2 DNA was found in different organs by PCR as well as by ISH.…”

mentioning

confidence: 99%

Herpesviral Hematopoietic Necrosis in Goldfish in Switzerland

et al. 2015

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Cyprinid herpesvirus 2 is a pathogen of goldfish, inducing a disease referred to as herpesviral hematopoietic necrosis. The disease is described so far in Japan, North America, Taiwan, Australia, the United Kingdom, and recently also Italy. Here the authors describe histologic lesions in clinically affected fish in comparison with clinically normal but virus DNA-positive goldfish in Switzerland. While necrosis or enhanced single-cell necrosis in the hematopoietic tissue in the pronephros or mesonephros was evident in dead and sick animals, in clinically normal goldfish, only single-cell necrosis was observed. Virus DNA was demonstrated in dead as well as clinically affected and subclinically infected goldfish by polymerase chain reaction and in situ hybridization. This study identifies the presence of goldfish herpesvirus in Switzerland and highlights the fact that the virus might be more widespread than assumed, as clinically normal goldfish can also carry cyprinid herpesvirus 2, showing histologically similar lesions but of lesser extent and severity.

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“…Gross pathology includes pale gills, and swollen spleen and kidney (Jeffery et al, 2007). The disease has been reported in the USA (Goodwin et al, 2006;Goodwin et al, 2009;Groff et al, 1998), Taiwan (Chang et al, 1999), Australia (Stephens et al, 2004), UK (Jeffery et al, 2007), and Hungary (Doszpoly et al, 2011b). CyHV-2 seems to be widespread on commercial goldfish farms, with outbreaks occurring when fish are subjected to stress during permissive temperatures (Goodwin et al, 2009).…”

Section: Carp Herpesvirusesmentioning

confidence: 99%

“…The causative agent of herpesviral haematopoietic necrosis of goldfish was first identified in the early 1990s in Japan (Jung & Miyazaki, 1995), and later designated Cyprinid herpesvirus 2 (CyHV-2). This virus may cause severe mortality especially among juvenile goldfish (Chang et al, 1999;Groff et al, 1998;Jeffery et al, 2007;Jung & Miyazaki, 1995), at water temperatures between 15 and 25 °C (Jeffery et al, 2007). Clinical signs include lethargy, anorexia and inappetence.…”

Section: Carp Herpesvirusesmentioning

confidence: 99%