A versatile, quantitative analytical method for pharmaceutical relevant lipids in drug delivery systems

Jeschek, Dominik; Lhota, Gabriele; Wallner, Jakob; Vorauer-Uhl, Karola

doi:10.1016/j.jpba.2015.11.020

Cited by 28 publications

(17 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Metal cations, such as the sodium ion present in DPPG and the quaternary ammonium ion in DPPC, can participate in reactions that produce a sharp decrease in viscosity [ 43 ]. Hydrolysis of phospholipids produces two main lysoforms, with the 1-acyl lysoform being predominant [ 44 ]. Higher levels of lipids reduce the surface tension of protein-containing solutions [ 45 ] and the electrical conductivity of aqueous solutions [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

Design and development of a biorelevant simulated human lung fluid

Hassoun

Royall

Parry

et al. 2018

Journal of Drug Delivery Science and Technology

View full text Add to dashboard Cite

Biorelevant fluids are required to enable meaningful in vitro experimental determinations of the biopharmaceutical properties of inhaled medicines, e.g. drug solubility, particle dissolution, cellular uptake. Our aim was to develop a biorelevant simulated lung fluid (SLF) with a well-defined composition and evidence-based directions for use. The SLF contained dipalmitoylphosphotidylcholine, dipalmitoylphosphatidylglycerol, cholesterol, albumin, IgG, transferrin and antioxidants. Freshly made SLF had pH 7.2, viscosity 1.138 × 10−3 Pa s, conductivity 14.5 mS/m, surface tension 54.9 mN/m and density 0.999 g/cm3. Colour, surface tension and conductivity were the most sensitive indicators of product deterioration. The simulant was stable for 24 h and 48 h at 37 °C and 21 °C, respectively, (in-use stability) and for 14 days when stored in a refrigerator (storage stability). To extend stability, the SLF was vacuum freeze-dried in batches to produce lyophilised powder that can be reconstituted readily when needed at the point of use. In conclusion, we have reported the composition and manufacture of a biorelevant, synthetic SLF, provided a detailed physico-chemical characterisation and recommendations for how to store and use a product that can be used to generate experimental data to provide inputs to computational models that predict drug bioavailability in the lungs.

show abstract

Section: Resultsmentioning

confidence: 99%

Design and development of a biorelevant simulated human lung fluid

Hassoun

Royall

Parry

et al. 2018

Journal of Drug Delivery Science and Technology

View full text Add to dashboard Cite

show abstract

“…To make sure that this analytical method could be applied for daily quality control along a manufacturing process, when consistent precisions is required, intra-and inter-day precisions were evaluated directly on a single nanoparticle suspension. The results obtained in these studies are presented in [22,41,54,85]. As part of the quantification process, repeatability and intermediate precision of the freeze-dried process were 0.37% and 0.52%, respectively.…”

Section: Repeatability and Intermediate Precision On Nanoparticle Susmentioning

confidence: 96%

“…Table 4, the repeatability of all analytes ranged from 0.9% to 5.3% and the accuracy from 83.5% to 112.2%. For lipid analysis repeatability, a value lower than 5% is generally accepted [22,41,54,85].…”

Section: Detection and Quantification Limitsmentioning

confidence: 99%

“…From an analytical point of view, the quality of these pharmaceuticals must be assured through the development of appropriate methods to characterize and quantify their lipid components and degradation products [18,19]. For this purpose, high-performance liquid chromatography (HPLC) is the technique of choice because of its ability to separate lipids into different classes or species based on their alkyl chain lengths and/or head group polarity [20][21][22][23]. For instance, reversed-phase (RP)-HPLC has become the most popular method for separating and analyzing triglyceride species in oils and fats, because it operates on the principle of both chain length and degree of unsaturation of fatty acids, thus enabling separation of individual triglyceride molecules [24,25].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development and validation of a novel UPLC-ELSD method for the assessment of lipid composition of nanomedicine formulation

Varache

Ciancone

Couffin

2019

International Journal of Pharmaceutics

View full text Add to dashboard Cite

This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Development and validation of a novel UPLC-ELSD method for the assessment of lipid composition of nanomedicine formulation.

show abstract

“…Moreover, powerful scavengers of freeradical oxidants result via H-atom donation as well as electron transfer, exerting their antioxidant activity. [48][49][50] However, the Fe 3 O 4 @OA-CS-5-FLU-NPs exhibited 5-FLU release at pH 5.2, indicating the nature of the loaded 5-FLU suggesting that the Fe 3 O 4 @OA-CS-5-FLU-NPs are proper carriers to deliver 5-FLU to targeted cancerous cell sites without early release.…”

Section: In Vitro Drug Release Studiesmentioning

confidence: 99%

<p>ROS-Responsive Chitosan Coated Magnetic Iron Oxide Nanoparticles as Potential Vehicles for Targeted Drug Delivery in Cancer Therapy</p>

Ayyanaar¹,

Balachandran²,

Bhaskar³

et al. 2020

IJN

View full text Add to dashboard Cite

Background and Objective: Cancer cells accumulate high concentrations of reactive oxygen species as a result of their faster and uninhibited metabolic activity. Cancer chemotherapeutic agents release an excess of severe adverse reactions as a result of targeting normal cells. This demands an improvement in targeted drug-delivery systems to selectively discharge anticancer drugs in the vicinity of such highly metabolically and mitotically active cells. Materials and Methods: Here, magnetic nanoparticles were synthesized by a traditional co-precipitation technique. Fe 3 O

show abstract

A versatile, quantitative analytical method for pharmaceutical relevant lipids in drug delivery systems

Cited by 28 publications

References 25 publications

Design and development of a biorelevant simulated human lung fluid

Design and development of a biorelevant simulated human lung fluid

Development and validation of a novel UPLC-ELSD method for the assessment of lipid composition of nanomedicine formulation

<p>ROS-Responsive Chitosan Coated Magnetic Iron Oxide Nanoparticles as Potential Vehicles for Targeted Drug Delivery in Cancer Therapy</p>

Contact Info

Product

Resources

About