A Versatile Monoclonal Antibody Specific to Human SERPINB5

Teoh, Sonia Soo Yee; Wang, Hong; Risbridger, Gail P.; Whisstock, James C.; Bird, Phillip I.

doi:10.1089/hyb.2012.0035

Cited by 3 publications

(5 citation statements)

References 26 publications

(28 reference statements)

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“…The MYBBP1A gene had the signature of positive selection in the ancestor of toothed whales, and is involved in p53 activation playing a fundamental role in programmed cell death 40 . In baleen whales we also found genes that could protect against the tumor growth, like the SERPINB5 gene, that has an important role in angiogenesis and metastasis 41 . Considering the evolution of the enormous body size in cetaceans, molecular variants in CXCR2, MYBBP1A and SERPINB5 could arise as a protective mechanism against the development of tumors.…”

Section: Tsgs With the Signature Of Positive Selection Are Related Tomentioning

confidence: 76%

Positive selection and gene duplications in tumour suppressor genes reveal clues about how cetaceans resist cancer

Tejada‐Martinez

Magalhães

Opazo

2020

Preprint

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Cetaceans are the longest-lived species of mammals and the largest in the history of the planet. They have developed mechanisms against diseases like cancer, however their underlying molecular and genetic basis remain unknown. The goal of this study was to investigate the role of natural selection in the evolution of tumor suppressor genes in cetaceans. We found signal of positive selection 29 tumor suppressor genes and duplications in 197 genes. The turnover rate of tumor suppressor genes was almost 6 times faster in cetaceans when compared to other mammals. Those genes with duplications and with positive selection are involved in important cancer regulation mechanisms (e.g. chromosome break, DNA repair and biosynthesis of fatty acids). They are also related with multiple ageing and neurological disorders in humans (e.g. Alzheimer, Nijmegen breakage syndrome, and schizophrenia). These results provide evolutionary evidence that natural selection in tumor suppressor genes could act on species with large body sizes and extended life span, providing insights into the genetic basis of disease resistance. We propose that the cetaceans are an important model in cancer, ageing and neuronal, motor and behavior disorders.

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Section: Tsgs With the Signature Of Positive Selection Are Related Tomentioning

confidence: 76%

Positive selection and gene duplications in tumour suppressor genes reveal clues about how cetaceans resist cancer

Tejada‐Martinez

Magalhães

Opazo

2020

Preprint

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“…Of the several that we tested, two efficiently recognize mouse maspin ( Supplementary Fig. 1 ): a monoclonal (13G11) arising from our previous hybridoma screen 16 and a commercial antibody (Santa Cruz H130) reported to recognize mouse maspin 17 . In immunoblotting experiments both antibodies reported the absence of maspin in adult knockout skin ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…For analysis of wt or knockout skin, mouse anti-maspin monoclonal antibody 13G11 (ref. 16 and Supplementary Fig. 6 ) was diluted 1:500 in 1% BSA ( w / v ) in PBS and incubated overnight on sections at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…For xenotransplant analysis, mouse anti-maspin monoclonal antibody 16F7 (ref. 16 ) was diluted 1:200 in 1% BSA ( w / v ) in PBS and incubated overnight on sections at room temperature. Bound antibody was detected by biotinylated rabbit anti-mouse IgG (Rockland) at 1:200 dilution for 30 min, followed by HRP-conjugated streptavidin (Chemicon) at 1:200 dilution for 30 min and visualized using the Liquid DAB Chromogen Substrate System (DAKO, Denmark).…”

Section: Methodsmentioning

confidence: 99%

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Maspin is not required for embryonic development or tumour suppression

et al. 2014

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Maspin (SERPINB5) is accepted as an important tumour suppressor lost in many cancers. Consistent with a critical role in development or differentiation maspin knockout mice die during early embryogenesis, yet clinical data conflict on the prognostic utility of maspin expression. Here to reconcile these findings we made conditional knockout mice. Surprisingly, maspin knockout embryos develop into overtly normal animals. Contrary to original reports, maspin re-expression does not inhibit tumour growth or metastasis in vivo, or influence cell migration, invasion or survival in vitro. Bioinformatic analyses reveal that maspin is not commonly under-expressed in cancer, and that perturbation of genes near maspin may in fact explain poor survival in certain patient cohorts with low maspin expression.

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“…For BRINP1 antibody production, rats were immunised with gel slices containing purified, denatured recombinant BRINP1 produced in a Baculovirus expression system. Antiserum was validated by indirect immunofluorescence of COS-1 cells transiently expressing human BRINP1 and immunoblotting of corresponding COS-1 cell lysates, following approaches described by Teoh et al [ 39 ]. No signal was detected in mock-transfected (control) COS-1 cell samples.…”

Section: Methodsmentioning

confidence: 99%

Brinp1 −/− mice exhibit autism-like behaviour, altered memory, hyperactivity and increased parvalbumin-positive cortical interneuron density

Berkowicz

Featherby

et al. 2016

Molecular Autism

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BackgroundBMP/RA-inducible neural-specific protein 1 (Brinp1) is highly conserved in vertebrates, and continuously expressed in the neocortex, hippocampus, olfactory bulb and cerebellum from mid-embryonic development through to adulthood.MethodsBrinp1 knock-out (Brinp1−/−) mice were generated by Cre-recombinase-mediated removal of the third exon of Brinp1. Knock-out mice were characterised by behavioural phenotyping, immunohistochemistry and expression analysis of the developing and adult brain.ResultsAbsence of Brinp1 during development results in a behavioural phenotype resembling autism spectrum disorder (ASD), in which knock-out mice show reduced sociability and changes in vocalisation capacity. In addition, Brinp1−/− mice exhibit hyper-locomotor activity, have impaired short-term memory, and exhibit poor reproductive success.Brinp1−/− mice show increased density of parvalbumin-expressing interneurons in the adult mouse brain. Brinp1−/− mice do not show signs of altered neural precursor proliferation or increased apoptosis during late embryonic brain development. The expression of the related neuronal migration genes Astn1 and Astn2 is increased in the brains of Brinp1−/− mice, suggesting that they may ameliorate the effects of Brinp1 loss.ConclusionsBrinp1 plays an important role in normal brain development and function by influencing neuronal distribution within the cortex. The increased cortical PV-positive interneuron density and altered behaviour of Brinp1−/− mice resemble features of a subset of human neurological disorders; namely autism spectrum disorder (ASD) and the hyperactivity aspect of attention deficit hyperactivity disorder (ADHD).Electronic supplementary materialThe online version of this article (doi:10.1186/s13229-016-0079-7) contains supplementary material, which is available to authorized users.

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A Versatile Monoclonal Antibody Specific to Human SERPINB5

Cited by 3 publications

References 26 publications

Positive selection and gene duplications in tumour suppressor genes reveal clues about how cetaceans resist cancer

Positive selection and gene duplications in tumour suppressor genes reveal clues about how cetaceans resist cancer

Maspin is not required for embryonic development or tumour suppression

Brinp1 −/− mice exhibit autism-like behaviour, altered memory, hyperactivity and increased parvalbumin-positive cortical interneuron density

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