A Versatile Method for the <i>in vitro</i> Perfusion of Isolated Organs of Rats and Mice with Particular Reference to Liver

Long-Term Perfusion of Isolated Rat Liver

“…The liver was isolated by the usual surgical technique (20) and the biliary duct was cannulated. About 5 -7 min elapsed between cannulating the portal vein and completing surgery, and starting perfusion in the apparatus; in the meantime, the medium was infused in situ.…”

Differential Effects of Fasting and Nicotinic Acid on Various Enzymatic Activities of the 9,000 <i>g</i> Fraction of the Perfused Rat Liver

“…The liver was isolated by the usual surgical technique and the biliary duct was cannulated (16). About 3 5 min elapsed between cannulation of the portal vein and completion of the surgery, when perfusion was started.…”

Effect of Diazepam on Liver Function of Rats

“…The technique to perfuse isolated ra t liver, the m ethod to collect bile in this experim ental condition and Ihe conditions to perform the BSP test have been described in a separate paper [ 10]. Diazepam (Noan, am p. obtained from ltavizza, Muggib), was added to the perfusion liquid at a concentration of 5 0 /zg/ml.…”

“…In the bile besides the volum e excreted each 30 m in, the bilirubine [12] and BSP excretions [13] w ere determ ined. At the end o f the perfusion the following determ inations were carried out on sam ples of liver tissue: proteins [11], gly cogen [8], cholesterol [1], triglycerides [14], phospholipids [9] and succinic dehydrogenase [ 10]. …”