A versatile assay for gelatinases using succinylated gelatin

Baragi, Vijaykumar M.; Shaw, Bonnie J.; Renkiewicz, Richard R.; Kuipers, Paul J.; Welgus, Howard G.; Mathrubutham, Mahesh; Cohen, Jon R.; Rao, Srinivasa K.

doi:10.1016/s0945-053x(00)00086-x

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Gelatin Zymography Assay2

Assays Using Succinylated Gelatin1

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Cited by 51 publications

(27 citation statements)

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“…Succinylated gelatin was primarily digested by MMP-2 and -9. The detection limit for catalytically active MMP-9 was 12.5 ng (1.0 nM) and that for MMP-2 was 20 ng (2 nM), using a 30 min incubation [10].…”

Section: Assays Using Succinylated Gelatinmentioning

confidence: 95%

Advances in assays of matrix metalloproteinases (MMPs) and their inhibitors

Cheng

Fang

2008

Journal of Enzyme Inhibition and Medicinal Chemistry

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Matrix metalloproteinases (MMPs) play an important role in many physiological and pathological processes. To assay the activities of MMPs is important in diagnosis and therapy of the MMPs associated diseases, such as neoplastic, rheumatic and cardiovascular diseases. Several assay systems have been developed, which include bioassay, zymography assay, immunoassay, fluorimetric assay, radio isotopic assay, phage-displayed assay, multiple-enzyme/multiple-reagent assay and activity-based profiling assay. The principle, application, advantage and disadvantage of these assays have been reviewed in this article.

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Section: Assays Using Succinylated Gelatinmentioning

confidence: 95%

Advances in assays of matrix metalloproteinases (MMPs) and their inhibitors

Cheng

Fang

2008

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…Gelatin zymography was performed as described previously [38]. Briefly, spleen cells were cultured in serum-free RPMI 1640 medium to exclude the effect of serum on the measurement of MMP.…”

Section: Gelatin Zymography Assaymentioning

confidence: 99%

Involvement of lymphocytes with a Th1 cytokine profile in bone cell damage associated with MMP-9 production in collagen-induced arthritis

Chen

Zhang

2004

Inflamm. res.

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“…All these compounds were assayed for enzymatic inhibitions against APN using Bestatin, a marked APNI, as the positive control. 12,13 Four compounds (2, 4, 17 and 19), with the IC 50 values lower than 100 lM, possess relatively good inhibitory activity against APN (the structures were shown in Fig. 2).…”

Section: Contents Lists Available At Sciverse Sciencedirectmentioning

confidence: 99%