A Validated Rp-HPLC Method for Simultaneous Estimation of Pyrantel Pamoate and Praziquantel in Bulk and Pharmaceutical Dosage Form

Rajesh, R; James, Jithu Jerin

doi:10.22159/ijpps.2019v11i5.30488

Cited by 6 publications

(1 citation statement)

References 13 publications

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“…13 The literature described quite a few analytical methods for the estimation of ivermectin and praziquantel in combination with other drugs. These methods are based on techniques, such as ultraviolet spectroscopy, 16 diffuse reflectance spectroscopy, 16 high performance liquid chromatography, [17][18][19][20][21] high performance thin layer chromatography 22 and high performance liquid chromatography with tandem mass spectrometry. 23 To the best of our knowledge, till now only one HPLC method for the simultaneous quantification of ivermectin and praziquantel in Equimax paste is reported.…”

Section: Discussionmentioning

confidence: 99%

Chromatographic Quantification of Ivermectin and Pranziquantel in the Tablets Using Stability Indicating RP-HPLC Method

Devaka

Rao

2019

Pharm Sci

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Background: A new stability indicating RP-HPLC based assay method was developed to quantify ivermectin and praziquantel simultaneously and applied effectively to tablets. Methods: The simultaneous assay of ivermectin and praziquantel by RP-HPLC was done using an YMC C18 (250 mm × 4.6 mm, 5 µm) column with a mobile phase mixture of 0.1M disodium hydrogen phosphate (pH 4.5) and acetonitrile (55:45, v/v) using a isocratic flow rate of 1.0 ml/min and measured at 242 nm using photodiode array detector. All parameters were validated following the ICH guiding principles. The method was applied to quantify ivermectin and praziquantel simultaneously in tablets. Results: The retention values of ivermectin and praziquantel were 3.465 min and 4.468 min, respectively. The method’s linearity was found to be 1-3 µg/ml (ivermectin) and 25-75 µg/ml (praziquantel). The limit of detection was 0.010 µg/ml (ivermectin) and 0.046 µg/ml (praziquantel); limit of quantification was 0.033 µg/ml (ivermectin) and 0.155 µg/ml (praziquantel). The percent relative standard deviation of ivermectin and praziquantel was ˂1.0%. The percent assay was 99.51% and 99.20% for ivermectin and praziquantel, respectively. In tablets, the percent recovery of ivermectin and praziquantel was 99.60% and 99.38% with a percent relative standard deviation value of 0.353% and 0.106%, respectively. Stability indicating capability of the method was demonstrated through the stress degradation studies. Conclusion: The developed method was proved to be selective, precise and accurate for the quality control of ivermectin and praziquantel in tablets.

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Section: Discussionmentioning

confidence: 99%

Chromatographic Quantification of Ivermectin and Pranziquantel in the Tablets Using Stability Indicating RP-HPLC Method

Devaka

Rao

2019

Pharm Sci

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Characterization of the major degradation products of the praziquantel API by mass spectrometry: Development and validation of a stability-indicating reversed phase UPLC method

Kumar

Chalannavar

2022

Journal of Liquid Chromatography & Related Technologies

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Veteri̇ner Dozaj Formunda İki̇ Antelmi̇nti̇k İlacin Eşzamanli Kanti̇tati̇f Anali̇zi̇

BÜKER

YAŞAR

Dinç

2022

Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji

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In this research article, a new chromatographic method ultra-performance liquid chromatography (UPLC) method was developed for the quantification of ivermectin (IMT)-preziquantel (PRZQ) in a two-component mixture and veterinary dosage form mixture. The separation of the studied veterinary drugs (IMT and PRZQ) was carried out on Waters Acquity ® BEH C18 column (50mm x 2.1 mm i.d., 1.7 m) using a mobile phase consisting of water-acetonitrile-methanol (10:70:20, v/v). In the chromatographic analysis, flow rate was 0.38 mL/min, and column temperature was maintained at 42 0C. The chromatographic detection of IMT and PRZQ was made at the wavelength of 220.0 and 245.0 nm, respectively. The developed UPLC method was validated and applied to real samples consisting of IMT and PRZQ. Recovery assay results were found to be 100.4 % for PRZQ and 101.8 % for IMT. The presented UPLC method is introduced as an alternative choice for the quality control of pharmaceutical preparations containing these drugs. For the quality control of pharmaceutical preparations containing these drugs, the presented UPLC method is introduced as an alternative experimental analysis choice.

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A Validated Rp-HPLC Method for Simultaneous Estimation of Pyrantel Pamoate and Praziquantel in Bulk and Pharmaceutical Dosage Form

Cited by 6 publications

References 13 publications

Chromatographic Quantification of Ivermectin and Pranziquantel in the Tablets Using Stability Indicating RP-HPLC Method

Chromatographic Quantification of Ivermectin and Pranziquantel in the Tablets Using Stability Indicating RP-HPLC Method

Characterization of the major degradation products of the praziquantel API by mass spectrometry: Development and validation of a stability-indicating reversed phase UPLC method

Veteri̇ner Dozaj Formunda İki̇ Antelmi̇nti̇k İlacin Eşzamanli Kanti̇tati̇f Anali̇zi̇

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