A validated HPLC – FLD method for the determination of mycotoxin levels in sun dried fermented cocoa beans: Effect of cola nut extract and powder

Kyei-Baffour, Vincent; Kongor, John Edem; Anyebuno, George; Budu, Agnes Simpson; Firibu, Saalia Kwesi; Afoakwa, Emmanuel Ohene

doi:10.1016/j.lwt.2021.111790

Cited by 10 publications

(4 citation statements)

References 24 publications

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“…Furthermore, most chromatographic methods require solid-phase pre-treatment and immunoaffinity techniques in order to reduce interferences and increase their efficiency in detection [ 15 ]. Despite their flaws, they were widely used, and HPLC is considered as the reference method for the qualitative and quantitative determination of mycotoxins [ 16 , 17 ]. However, because of the complicated and time-demanding sample preparation that is required in HPLC, immunological methods like ELISA are preferred in research studies and in routine analyses [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Evaluation of Three Commercial Elisa Kits Used for the Detection of Aflatoxins B1, B2, G1, and G2 in Feedstuffs and Comparison with an HPLC Method

Maggira¹,

Sakaridis²,

Ioannidou³

et al. 2022

Veterinary Sciences

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Various analytical techniques for detecting mycotoxins have been developed in order to control their concentration in food and feed. Conventional analytical approaches for mycotoxin identification include thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and gas chromatography (GC). Rapid methods for mycotoxin analysis are also becoming increasingly relevant. One of the most common rapid methods for determining these compounds is the enzyme-linked immunosorbent assay (ELISA). The current study aimed to compare three available ELISA kits for the detection and quantification of aflatoxins B1, B2, G1, and G2 in spiked feed samples at known quantities. All three ELISA kits were validated and showed good performance with high recovery rates and LOD and LOQ values lower than the MRL. The developed HPLC-FL method was validated for all the compounds determining the accuracy, precision, linearity, decision limit, and detection capability with fairly good results. Unknown feed samples (corn, silage, pellet, barley, wheat, soya, and sunflower) were also tested using the best ELISA kit and HPLC, and the results were compared. Both ELISA and HPLC were proven to be suitable methods for mycotoxin analysis. The analytical technique should be determined primarily by the availability and number of samples.

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Section: Introductionmentioning

confidence: 99%

Comparative Evaluation of Three Commercial Elisa Kits Used for the Detection of Aflatoxins B1, B2, G1, and G2 in Feedstuffs and Comparison with an HPLC Method

Maggira¹,

Sakaridis²,

Ioannidou³

et al. 2022

Veterinary Sciences

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“…Nowadays, several analytical methods have been developed for the determination of AFB 1 , such as enzyme-linked immunosorbent assay (ELISA), spectrophotometry, and chromatography methods. , The spectrophotometry and chromatography techniques include gas chromatography–mass spectrometry, high-performance liquid chromatography–fluorescence detector, and high-performance liquid chromatography–mass spectrometry. − Nevertheless, due to the low residue level of AFB 1 in the complex food matrices, the high accuracy determination of AFB 1 in these matrices is still challenging using the abovementioned techniques. For example, on the basis of reports about the proficiency test (PT) from the European Union Reference Laboratories, about 10% of the participants reported unsatisfactory results in the case of mycotoxin analysis .…”

Section: Introductionmentioning

confidence: 99%

Development of a Matrix Certified Reference Material for Quantification of Aflatoxin B₁ in Peanut Oils

Zhao,

Li,

et al. 2024

ACS Food Sci. Technol.

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Aflatoxin B 1 in a peanut oil matrix certified reference material (CRM, GBW10176) has been issued for accurate determination of aflatoxin B 1 in real samples. This matrix CRM was prepared from commercial peanut oils naturally contaminated by aflatoxin B 1 , with the aid of a liquid−liquid extraction process. Specially, the certified value of CRM was determined using two individual approaches, liquid chromatography−isotope dilution tandem mass spectrometry and standard addition liquid chromatography−fluorescence detection techniques. More importantly, assessments of homogeneity and short-and long-term stabilities confirmed that the GBW10176 CRM was sufficiently homogeneous between packages or within package, and it could be stable up to 7 days stored below 50 °C or 18 months at 4 °C. The metrological traceability of GBW10176 was ensured by the application of pure aflatoxin B 1 (GBW10172). Its uncertainty was evaluated with the contributions from the characterization, homogeneity, and stability. Resultantly, the certified value of the aflatoxin B 1 CRM (GBW10176) was 23.4 μg/kg with an uncertainty of 3.1 μg/kg (k = 2).

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“… 85 Ochratoxins Penicillium sp. 85 Cocoa beans Aflatoxins A. flavus 86 Ochratoxins A. carbonarius 86 Animal feed Aflatoxins A. flavus 84 …”

Section: Introductionmentioning

confidence: 99%

Survey of knowledge, and attitudes to storage practices preempting the occurrence of filamentous fungi and mycotoxins in some Ghanaian staple foods and processed products

Kortei,

Badzi,

Nanga

et al. 2023

Sci Rep

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Mycotoxigenic fungi can infect and produce potent mycotoxins in foodstuffs prior to harvest, during harvest (field fungi), and in storage after harvest (storage fungi), which when ingested, can result in adverse health effects. This study was aimed at assessing the knowledge, attitudes, and practices adopted by the Ghanaian populace to help mitigate the occurrence of molds and mycotoxins in foods. A cross-sectional survey involving a structured questionnaire was conducted with 642 respondents from twelve regions of Ghana. Descriptive statistics and analyses of variance were calculated. Correct Classification Rate (CCR) was measured to assess the utility of a logistic regression model. The results of the study showed that the majority of 299 (46.6%) of the respondents were between the ages of 18–25. Age and educational level were related to knowledge about the occurrence of fungi and mycotoxins in foods (p < 0.05). More than half the respondents, 50% indicated that they knew of aflatoxins as a major mycotoxin present in food. Higher education directly influenced on the knowledge of mycotoxicosis and the management of stored food to present intoxication by fungal metabolites. 502 (32.9%) knew that consuming foods with toxins could cause stomach aches. The most commonly consumed food commodity despite the presence of visible growth of fungi was bread (35.3%). The average KAP score for knowledge showed that, out of 100%, there was adequate knowledge (63.8%) among the members of the Ghanaian populace. Favorable environmental conditions of high humidity (> 85% ERH) and temperature (> 28–32 °C) enhance the proliferation of fungi in most foods and the attendant production of mycotoxins such as aflatoxins, ochratoxins, and fumonisins are associated with several severe human and animal health conditions; mycotoxicosis was associated with high fever, pain, vomiting, suppression of immunity, cancer, etc. when these foods are consumed on regular basis for a prolonged length of time. Future examination of the food items used for the School Feeding Programme in Ghana will offer opportunities to examine the risks of feeding youth with fungal-contaminated food preparations from providers.

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A validated HPLC – FLD method for the determination of mycotoxin levels in sun dried fermented cocoa beans: Effect of cola nut extract and powder

Cited by 10 publications

References 24 publications

Comparative Evaluation of Three Commercial Elisa Kits Used for the Detection of Aflatoxins B1, B2, G1, and G2 in Feedstuffs and Comparison with an HPLC Method

Comparative Evaluation of Three Commercial Elisa Kits Used for the Detection of Aflatoxins B1, B2, G1, and G2 in Feedstuffs and Comparison with an HPLC Method

Development of a Matrix Certified Reference Material for Quantification of Aflatoxin B₁ in Peanut Oils

Survey of knowledge, and attitudes to storage practices preempting the occurrence of filamentous fungi and mycotoxins in some Ghanaian staple foods and processed products

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A validated HPLC – FLD method for the determination of mycotoxin levels in sun dried fermented cocoa beans: Effect of cola nut extract and powder

Cited by 10 publications

References 24 publications

Comparative Evaluation of Three Commercial Elisa Kits Used for the Detection of Aflatoxins B1, B2, G1, and G2 in Feedstuffs and Comparison with an HPLC Method

Comparative Evaluation of Three Commercial Elisa Kits Used for the Detection of Aflatoxins B1, B2, G1, and G2 in Feedstuffs and Comparison with an HPLC Method

Development of a Matrix Certified Reference Material for Quantification of Aflatoxin B1 in Peanut Oils

Survey of knowledge, and attitudes to storage practices preempting the occurrence of filamentous fungi and mycotoxins in some Ghanaian staple foods and processed products

Contact Info

Product

Resources

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Development of a Matrix Certified Reference Material for Quantification of Aflatoxin B₁ in Peanut Oils