A vacuolar H+-pyrophosphatase differential activation and energy coupling integrate the responses of weeds and crops to drought stress

Venancio, Josimara Barcelos; Catunda, Michelle Guedes; Ogliari, Juares; Rima, Janaína Aparecida Hottz; Okorokova‐Façanha, Anna L.; Okorokov, Lev A.; Façanha, Arnoldo Rocha

doi:10.1016/j.bbagen.2013.12.019

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…However, we only observed these features when comparing inoculated plants (Figure 7). Also, the reduced hydrolytic activity of those pumps observed in inoculated AVP1OX rice (Figure 6) demonstrates an enhanced efficiency of H + pumps’ coupling (Figure 8), as previously observed in plants exposed to stress (Gaxiola et al, 2001; Venancio et al, 2014; Schilling et al, 2017). This observation supports the idea that although plant inoculation with PGPM results in increased plant development, the inoculation is associated with a moderated biotic stress, which activates defense mechanisms and stimulates the plant (Shrivastava et al, 2018; Xu et al, 2018).…”

Section: Discussionsupporting

confidence: 79%

Inoculation With Piriformospora indica Is More Efficient in Wild-Type Rice Than in Transgenic Rice Over-Expressing the Vacuolar H+-PPase

et al. 2019

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Achieving food security in a context of environmental sustainability is one of the main challenges of the XXI century. Two competing strategies to achieve this goal are the use of genetically modified plants and the use of plant growth promoting microorganisms (PGPMs). However, few studies assess the response of genetically modified plants to PGPMs. The aim of this study was to compare the response of over-expressing the vacuolar H + -PPase (AVP) and wild-type rice types to the endophytic fungus; Piriformospora indica . Oryza sativa plants (WT and AVP) were inoculated with P. indica and 30 days later, morphological, ecophysiological and bioenergetic parameters, and nutrient content were assessed. AVP and WT plant heights were strongly influenced by inoculation with P. indica , which also promoted increases in fresh and dry matter of shoot in both genotypes. This may be related with the stimulatory effect of P. indica on ecophysiological parameters, especially photosynthetic rate, stomatal conductance, intrinsic water use efficiency and carboxylation efficiency. However, there were differences between the genotypes concerning the physiological mechanisms leading to biomass increment. In WT plants, inoculation with P. indica stimulated all H + pumps. However, in inoculated AVP plants, H + -PPase was stimulated, but P- and V-ATPases were inhibited. Fungal inoculation enhanced nutrient uptake in both shoots and roots of WT and AVP plants, compared to uninoculated plants; but among inoculated genotypes, the nutrient uptake was lower in AVP than in WT plants. These results clearly demonstrate that the symbiosis between P. indica and AVP plants did not benefit those plants, which may be related to the inefficient colonization of this fungus on the transgenic plants, demonstrating an incompatibility of this symbiosis, which needs to be further studied.

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Section: Discussionsupporting

confidence: 79%

Inoculation With Piriformospora indica Is More Efficient in Wild-Type Rice Than in Transgenic Rice Over-Expressing the Vacuolar H+-PPase

et al. 2019

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“…They were hulled at the beginning of cultivation because they took advantage more efficiently of the environmental factors available: high temperature and luminosity in a freshly prepared soil. These weeds have a C 4 photosynthetic cycle (Christin et al, 2014;Venâncio et al, 2014), which gives them higher photosynthetic rates compared to C 3 plants in those environmental conditions. The lowest densities occurred from 56 DAT (18.2 plants m -2 ) due to the weeds intraspecific and interspecific competition (Silva & Durigan, 2009) …”

Section: Resultsmentioning

confidence: 99%

Phytosociological Survey and Weed Interference in Eggplants Cultivation1

et al. 2016

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-Eggplants are vegetables of great importance in the South and Southeast regions of Brazil, which are subject to interference from weeds. In order to collaborate with weed management in this vegetable, this study was carried out to determine the periods of weed interference in growing eggplants. The study was conducted in the Brazilian municipality of Jaboticabal, SP, at the Faculty of Agricultural and Veterinary Sciences, UNESP [Universidade Estadual Paulista "Júlio de Mesquita Filho" (São Paulo State University)], in the period from February to July 2013. The experiment was arranged in a randomized block design with 22 treatments, three replications divided in 11 periods of coexistence and weed control with eggplants: 14, 28, 42, 56, 70, 84, 98, 112, 126, 140 and 154 days after transplanting (DAT). In each treatment, a phytosociological study was carried out to identify the important species of weeds in eggplant crops. The main weeds were Eleusine indica, Nicandra physaloides, Portulaca oleracea and Cyperus rotundus due to their high relative importance. The interference of these weeds caused losses of up to 96% to the standard commercial fruit yield. The period before interference was 6 DAT and the total period of interference was 102 DAT. Therefore, it is concluded that after the initial six days after transplanting eggplants, the crop should be free of weeds within the next 96 days.Keywords: Solanum melongena, competition, productivity, weed community. Nápoli: 14, 28, 42, 56, 70, 84, 98, 112, 126, 140 Palavras-chave: Solanum melongena, competição, produtividade, comunidade infestante. RESUMO -A berinjela é uma hortaliça de grande importância nas regiões Sul e Sudeste e, que está sujeita a interferências das plantas daninhas. Com o intuito de colaborar com o manejo das plantas daninhas nessa hortaliça, objetivou-se determinar os períodos de interferência das plantas daninhas no cultivo de berinjela Nápoli. O estudo foi realizado no município de Jaboticabal, SP, na Faculdade de Ciências Agrárias e Veterinárias da UNESP, no período de fevereiro a julho de 2013, no delineamento em blocos ao acaso com 22 tratamentos e três repetições, divididos em 11 períodos de convivência e de controle das plantas daninhas com a berinjela

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“…Specific inhibitors, including 0.2 mM vanadate (Na 3 VO 4 ; Sigma-Aldrich) and 5 nM concanamycin a (Sigma-Aldrich), were used to determine the hydrolytic activities of P-H + -ATPase and V-H + -ATPase, respectively. PPi hydrolysis, performed by H + -PPase, was determined through its dependence on K + …”

Section: Methodsmentioning

confidence: 99%

Embryogenic Competence Acquisition in Sugar Cane Callus Is Associated with Differential H⁺-Pump Abundance and Activity

et al. 2018

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Somatic embryogenesis is an important biological process in several plant species, including sugar cane. Proteomics approaches have shown that H pumps are differentially regulated during somatic embryogenesis; however, the relationship between H flux and embryogenic competence is still unclear. This work aimed to elucidate the association between extracellular H flux and somatic embryo maturation in sugar cane. We performed a microsomal proteomics analysis and analyzed changes in extracellular H-flux and H-pump (P-H-ATPase, V-H-ATPase, and H-PPase) activity in embryogenic and non-embryogenic callus. A total of 657 proteins were identified, 16 of which were H pumps. We observed that P-H-ATPase and H-PPase were more abundant in embryogenic callus. Compared to non-embryogenic callus, embryogenic callus showed higher H influx, especially on maturation day 14, as well as higher H-pump activity (mainly, P-H-ATPase and H-PPase activity). H-PPase appears to be the major H pump in embryogenic callus during somatic embryo formation, functioning in both vacuole acidification and PPi homeostasis. These results provide evidence for an association between higher H-pump protein abundance and, consequently, higher H flux and embryogenic competence acquisition in the callus of sugar cane, allowing for the optimization of the somatic embryo conversion process by modulating the activities of these H pumps.

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A vacuolar H+-pyrophosphatase differential activation and energy coupling integrate the responses of weeds and crops to drought stress

Cited by 7 publications

References 35 publications

Inoculation With Piriformospora indica Is More Efficient in Wild-Type Rice Than in Transgenic Rice Over-Expressing the Vacuolar H+-PPase

Inoculation With Piriformospora indica Is More Efficient in Wild-Type Rice Than in Transgenic Rice Over-Expressing the Vacuolar H+-PPase

Phytosociological Survey and Weed Interference in Eggplants Cultivation1

Embryogenic Competence Acquisition in Sugar Cane Callus Is Associated with Differential H⁺-Pump Abundance and Activity

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A vacuolar H+-pyrophosphatase differential activation and energy coupling integrate the responses of weeds and crops to drought stress

Cited by 7 publications

References 35 publications

Inoculation With Piriformospora indica Is More Efficient in Wild-Type Rice Than in Transgenic Rice Over-Expressing the Vacuolar H+-PPase

Inoculation With Piriformospora indica Is More Efficient in Wild-Type Rice Than in Transgenic Rice Over-Expressing the Vacuolar H+-PPase

Phytosociological Survey and Weed Interference in Eggplants Cultivation1

Embryogenic Competence Acquisition in Sugar Cane Callus Is Associated with Differential H+-Pump Abundance and Activity

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Embryogenic Competence Acquisition in Sugar Cane Callus Is Associated with Differential H⁺-Pump Abundance and Activity