A UV spectrophotometric method for the determination of folic acid in pharmaceutical tablets and dissolution tests

Abstract

Folic acid (FA) or pteroyl-L-glutamic acid, chemically known as N-[4-[[(2-amino-1,4-dihydro-4-oxo-6pteridinyl)methyl]amino]benzoyl]-L-glutamic acid, is a water-soluble B vitamin that helps build healthy cells. In this study, a new, simple, easy, accurate, precise, economic and sensitive UV spectrophotometric method for the determination of folic acid in commercial tablets and during dissolution tests is presented. Using a phosphate buffer solution at pH 9.0 it was possible to determine the concentration of folic acid in commercial tablets at a l max of 282.5 nm in a linear range of 1.0-17.5 mg ml À1 with a R 2 > 0.9999 and recovery between 100.6 and 101.1%. The method was also optimized for the quantification of folic acid in dissolution tests in the same linear concentration range. The pH effect on the UV spectra of folic acid solutions was eliminated with a first derivative. This procedure allowed the direct measurement of samples from the dissolution vessel (pH approximately equal to 6) without any pH correction. This method was based on the first derivative of the absorbance at l ¼ 377.2 nm, yielding an R 2 > 0.9997 with recovery percentages similar to those presented before. The statistical comparison between the proposed and the HPLC reference method showed excellent agreement and indicates no statistically significant difference in accuracy and precision at a 0.05 significance level.

Evaluation of Surfactants-Assisted Folic Acid-Loaded Pectin Submicrospheres: Characterization and Hemocompatibility Assay

“…Pectin submicrospheres behave as hydrophilic matrices whose release ability is currently related to their swelling ability in dissolution media. This result considered of good prognostic in obtaining further sustained drug delivery and controlled erosion/degradation with time and pH [41].…”

Evaluation of Bacterial Strains for the Induction of Plant Biochemicals, Nutritional Contents and Isozymes in Barley

“…It was mixed well, left for 2 hrs and then 10 mL of ethanol was added to the mixture. Absorbance was taken at 282 nm which was then used to calculate the quantity of folic acid with the help of standard curve made by using different concentration of ethanol [29].…”

In-line ATR-UV and Raman Spectroscopy for Monitoring API Dissolution Process During Liquid-Filled Soft-Gelatin Capsule Manufacturing

“…UV spectroscopy has been widely used in the pharmaceutical industry for the assessment of API potency, dissolution testing of dosage forms, and cleaning validation in a laboratory environment (15,16). Traditionally this is done by bringing manually prepared samples to UV spectrometer or by interfacing the UV detector with a high-performance liquid chromatography (HPLC) equipment.…”