A universal reagent for phospholipid analysis

Vaskovsky, V.E.; Kostetsky, E. Ya.; Vasendin, I.M.

doi:10.1016/s0021-9673(00)85249-8

Cited by 568 publications

(208 citation statements)

References 58 publications

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“…DOPC and lyso-PC concentrations were determined by phosphorus assay. 24) Cell viability was assayed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay kit (Sigma-Aldrich) according to the manufacturer's instructions. The fluorescence of cells after treatment with 18 : 1 PE-CFcontaining NPs was observed using Motic BA410 (400×) fluorescence microscope (Hong Kong).…”

Section: )mentioning

confidence: 99%

Cargo-Free Nanoparticles Containing Cationic Lipids Induce Reactive Oxygen Species and Cell Death in HepG2 Cells

Yun

Bae

Ahn

2016

Biological & Pharmaceutical Bulletin

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These effects were dependent on the lipid concentration, surface density of cationic lipids, and particle size of NPs. However, neutral NPs consisting of 1,2-dioleoyl-3-phosphocholine did not elicit the effective ROS generation or cell death regardless of the lipid concentration and particle size. The present study suggests that DOTAP-and DOTMA-NPs are able to induce cancer cell death through production of ROS in the absence of any therapeutic cancer reagents. These results also provide a rational background for the design of delivery systems using cationic lipid-based NP formulations.

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Section: )mentioning

confidence: 99%

Cargo-Free Nanoparticles Containing Cationic Lipids Induce Reactive Oxygen Species and Cell Death in HepG2 Cells

Yun

Bae

Ahn

2016

Biological & Pharmaceutical Bulletin

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“…MGDG, DGDG, and SQDG were using for constructed of calibration curves. PL was quantified based on the content of phosphorus using molybdate reagent [46]. Neutral lipids were analyzed by TLC on the 12 × 12 cm plates covered with silica gel 60F254 in developing solvent mixture hexane/diethyl ether/ acetic acid 80:20:1 (v/v/v).…”

Section: Lipids Analysismentioning

confidence: 99%

Seasonal Composition of Lipids, Fatty Acids Pigments in the Brown Alga <i>Sargassum pallidum</i>: The Potential for Health

Gerasimenko¹,

Logvinov²

2016

OJMS

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The present study describes the content of total lipids (TL), the main components of polar and neutral lipids (NL) and photosynthetic pigments (PSP) in edible seaweed Sargassum pallidum (Ochrophyta) from the Sea of Japan, Russia, in different months of the year, taking into account life cycle. Special attention was paid to the fatty acids (FAs) of total lipids as well as the accumulation of important polyunsaturated fatty acids (PUFAs) in glyceroglycolipids (GL) and reserve lipids in the seasonal cycles of growth. The content of TL strongly varied in the course of a year. The major lipid components were GL (20.3% -36.4%) and NL (19.8% -30.6%), while phospholipids (PL) were present in negligible amounts (3.2% -6.9%). Significant seasonal variations were observed in content all classes of lipids. The PUFAs prevailed in total lipids and the content of n-6 PUFAs was higher than n-3 PUFAs on all stages of life with the exception of vegetation period. Monogalactosyldiacylglycerols (MGDG) and digalactosyldiacylglecerols (DGDG) were rich in PUFAs and n-3 PUFAs were dominated among them independently of stages of growth. Saturated fatty acids (SFA) were dominated in sulfoquinovosyldiacylglycerols (SQDG). Triacylglycerols (TAG) and diacylglycerols (DAG) contained many PUFAs, especially n-6 PUFAs. Significant concentration 20:3 n-6 was in the triacylglycerols. The concentration of chlorophylls and carotenoids was increased with the growth and maturation of S. pallidum and reached maximum in period of highest day length and water temperature. The content of pigments decreased with the onset of short days and decreasing water temperatures. Nutritional value and benefit for health of human this alga were evaluated. The n-6/n-3 PUFAs ratio, nutritional quality index (atherogenic and thrombogenic indices, and ratio between hypocholesterolemic and hypercholesterolemic fatty acids) were low, suggesting a high nutritional value of S. pallidum throughout the year.

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“…Detection of phospholipids was carried out by the method of Vaskovsky et al [17]. First, source reagent A was prepared as follows: 0.4 g of hydrochloric acid hydrazine in 14 mL of 4n =Cl were added to 10 g of sodium molybdate in 60 mL of 4n HCl.…”

Section: Detection Of Lipidsmentioning

confidence: 99%

Effect of hypoxia on the composition and state of lipids and oxygen-transport properties of erythrocyte haemoglobin

Ревин

Grunyushkin

Gromova

et al. 2016

Biotechnology & Biotechnological Equipment

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The aim of this study was to investigate the relationship between the composition and state of erythrocytic lipids and oxygen-binding properties of erythrocyte haemoglobin-derived haematoporphyrin under hypoxia. The results from this study showed that hypoxia causes a profound change in the composition of the lipids that form the bilayer of erythrocyte membranes, as well as in the functional characteristics of erythrocytes. The detected changes correlate with morphological reconstruction of erythrocytes and, consequently, affect the oxygen-transport properties of erythrocytes. The obtained results showed that the lipid phase plays an important role in the functioning of erythrocytes in health as well as in event of development of ischemic heart disease and other pathological processes that accompany hypoxia.

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A universal reagent for phospholipid analysis

Cited by 568 publications

References 58 publications

Cargo-Free Nanoparticles Containing Cationic Lipids Induce Reactive Oxygen Species and Cell Death in HepG2 Cells

Cargo-Free Nanoparticles Containing Cationic Lipids Induce Reactive Oxygen Species and Cell Death in HepG2 Cells

Seasonal Composition of Lipids, Fatty Acids Pigments in the Brown Alga <i>Sargassum pallidum</i>: The Potential for Health

Effect of hypoxia on the composition and state of lipids and oxygen-transport properties of erythrocyte haemoglobin

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A universal reagent for phospholipid analysis

Cited by 568 publications

References 58 publications

Cargo-Free Nanoparticles Containing Cationic Lipids Induce Reactive Oxygen Species and Cell Death in HepG2 Cells

Cargo-Free Nanoparticles Containing Cationic Lipids Induce Reactive Oxygen Species and Cell Death in HepG2 Cells

Seasonal Composition of Lipids, Fatty Acids Pigments in the Brown Alga &lt;i&gt;Sargassum pallidum&lt;/i&gt;: The Potential for Health

Effect of hypoxia on the composition and state of lipids and oxygen-transport properties of erythrocyte haemoglobin

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Seasonal Composition of Lipids, Fatty Acids Pigments in the Brown Alga <i>Sargassum pallidum</i>: The Potential for Health