A unique microRNA profile in end-stage heart failure indicates alterations in specific cardiovascular signaling networks.

Abstract

This article has been withdrawn by the authors. Evaluation by the journal with image analysis software determined that in Fig. 5A, lanes 1-3 of the RB1 immunoblot were duplicated in lanes 4 -6, lane 4 of the ERBB2 immunoblot was duplicated in lane 6, lane 5 of the STAT3 immunoblot was duplicated in lane 8, and lanes 1-3 of the actin immunoblot were flipped horizontally and reused in lanes 6 -8. The authors state that RB1, ERRB2, STAT3, and actin in Fig. 5A were created from phosphor-chemiluminescent digital imaging. The authors also state that they have replicate data supporting the conclusions of Fig. 5, A and B. In Fig. 6B, evaluation by the journal of the original data determined that single cell background fluorescence was duplicated. The authors maintain that the concern is about a single background cell not a positive cell to show transfection efficiency and is inconsequential to proving transfection.

Circulating microRNAs as novel biomarkers for dilated cardiomyopathy

“…MiRNAs have been shown to be key molecular players in nearly all cellular processes, including cardiovascular development and pathophysiology [6,7]. Gain or loss of function experiments, expression arrays and bioinformatic analysis confirmed that specific miRNAs played essential roles in the biogenesis of DCM [6,[8][9][10][11][12][13]. This observation laid the theoretical foundation of the diagnostic application of miRNAs to DCM.…”

“…To test whether differentially expressed miRNAs of plasma from DCM patients mimic the pattern of cardiac miRNAs in end-stage HF because of DCM, 9 miRNAs representing the cardiac miRNA signature were assessed [10]. Surprisingly, we observed that 3 miRNAs (miR-1, miR-342, and miR-145) were barely detectable in the microarray and that none of the 6 remaining miRNAs significantly mirror the cardiac miRNA expression pattern (Fig.…”

Functional Network Analysis Reveals Versatile MicroRNAs in Human Heart

“…Although the miRNAs analyzed here was found significantly changed in human MI or HF [13][14][15], we still evaluated the statistical significance of the association between these miRNAs and MI or HF, with the introduced relevance score to quantity the miRNA-disease association. Relevance score assessed the potential functional association between a miRNA and disease by calculating the ratio of the common genes shared by miRNA target set and disease related gene set (See Materials and Methods).…”

“…To assess the regulatory capacity of each miRNA significantly dysregulated in HF and MI [13][14][15], the Cytoscape plugin NetworkAnalyzer [22] was firstly used to analyze the topological feature of its literaturereported [23] and predicted target genes [24][25][26][27] in three independent target gene sets (miRSel+MicroCosm, miRSel+PicTar, and miRSel+TargetScanHuman). NetworkAnalyzer is a built-in plugin for calculating network topological parameters in Cytoscape.…”

Epigenetic Modifications the Development of Different Heart Failure Phenotypes

“…On this way, signature of miRNA expression (especially miRNA-7, miRNA-123, miRNA-378) has been allowed to differ healthy and failing hearts and depended on reactivation of a fetal gene program. Indeed, these miRNAs have been displayed different expression levels in HF at early and end stage failing hearts [36]. However, low number of direct clinical evidence regarding specifically HF phenotypes' development relating to miRNA signature remains a part of scientific discussion [37].…”