A UHPLC-QTOF-MS/MS method for the simultaneous determination of eight triterpene compounds from Poria cocos (Schw.) Wolf extract in rat plasma: Application to a comparative pharmacokinetic study

Qi, Qian; Zhou, Na; Pengcheng, Qi; Zhang, Yuqian; Mu, Xiyan; Shi, Xiaowei; Wang, Qiao

doi:10.1016/j.jchromb.2018.10.011

Cited by 31 publications

(20 citation statements)

References 30 publications

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“…All the constituents identified in our present study were in agreement with those identified in previous reports [ 9 , 16 , 26 , 27 ]. Polyporenic acid C inhibits M-CSF and RANKL-induced osteoclastogenesis in BMMs [ 28 ], while pachymic acid enhances odontoblast differentiation and dentin mineralization via induction of heme oxygenase-1 [ 29 ].…”

Section: Resultssupporting

confidence: 93%

“…As shown in Figure 3 and Table 1, the PC used in our study contained 10 triterpenoids, including 6α-hydroxypolyporenic acid C, dehydrotumulosic acid, poricoic acid A, polyporenic acid C, 3-epidehydrotumulosic acid, dehydropachymic acid, pachymic acid, dehydrotrametenolic acid, dehydroeburicoic acid, and eburicoic acid, as determined using mass spectra and retention times. All the constituents identified in our present study were in agreement with those identified in previous reports [9,16,26,27]. Polyporenic acid C inhibits M-CSF and RANKL-induced osteoclastogenesis in BMMs [28], while pachymic acid enhances odontoblast differentiation and dentin mineralization via induction of heme oxygenase-1 [29].…”

Section: Phytochemical Constituents Of Pcsupporting

confidence: 92%

“…Therefore, the beneficial effects of PC on estrogen-deprivation-induced bone loss may be due to the activity of the constituents mentioned above. In addition, numerous pharmacokinetic studies have reported that dehydrotumulosic acid, poricoic acid A, polyporenic acid C, dehydropachymic acid, pachymic acid, and dehydrotrametenolic acid are absorbed, distributed, metabolized, and excreted after oral administration of PC [9,26,30]. Further studies are needed to elucidate the precise mechanisms of components responsible for the anti-osteoporotic activity shown by PC and its effects on bone homeostasis.…”

Section: Phytochemical Constituents Of Pcmentioning

confidence: 99%

“…In East Asian and European countries, the sclerotum of P. cocos has commonly been used therapeutically against various diseases, and as a functional food and dietary ingredient in tea, bread, and other baked goods [ 8 ]. P. cocos exerts beneficial effects on patients with insomnia, heart disorders, chronic edema, and nephrosis [ 9 ]. Recent pharmacological studies show that P. cocos and its constituents including polysaccharides possesses immunomodulatory, anti-inflammatory, anti-cancer, anti-hyperglycemic, and anti-nephritic activity [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Poria Cocos Ameliorates Bone Loss in Ovariectomized Mice and Inhibits Osteoclastogenesis In Vitro

et al. 2020

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Estrogen deprivation in postmenopausal women causes disruption of bone homeostasis, resulting in bone loss and osteoporosis. Conventional therapies can exert adverse effects. The sclerotum of Poria cocos has been used in traditional medicine and as a nutritional supplement and to treat various diseases. However, the effects of P. cocos on the bone remain largely undetermined. In this study, we examined the effects of P. cocos hydroethanolic extract (PC) on osteoclast differentiation and estrogen-deprivation-induced bone loss in an ovariectomized mouse model of postmenopausal osteoporosis. PC-mediated inhibition of receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast formation and resorption activity suppressed RANKL-induced expression of nuclear factor of activated T cells cytoplasmic 1 (NFATc1), which is a crucial transcription factor for osteoclast differentiation. In ovariectomized mice, PC markedly alleviated trabecular bone loss and reduced the accumulation of lipid droplets in the bone marrow. We additionally identified ten triterpenoid constituents of PC using UPLC-MS/MS analysis. Our results indicate that PC negatively regulated osteoclast differentiation and function, and can potentially be used to manage postmenopausal osteoporosis.

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Section: Resultssupporting

confidence: 93%

Section: Phytochemical Constituents Of Pcsupporting

confidence: 92%

Section: Phytochemical Constituents Of Pcmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Poria Cocos Ameliorates Bone Loss in Ovariectomized Mice and Inhibits Osteoclastogenesis In Vitro

et al. 2020

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“…In our previous pharmacokinetics studies of JWKXS and other research group studies, most of these compounds also could be detected in plasma of rats (Shi et al, 2018). Based on these research results, we selected a series of chemical compound with definite neuronal activities to develop the quality control method of JWKXS and optimized the preparation procedures, which set a consolidated basis for the drug development (Zhu et al, 2010; Cheng and Tsai, 2016; Qian et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Jia-Wei-Kai-Xin-San, an Herbal Medicine Formula, Ameliorates Cognitive Deficits via Modulating Metabolism of Beta Amyloid Protein and Neurotrophic Factors in Hippocampus of Aβ1-42 Induced Cognitive Deficit Mice

et al. 2019

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Jia-Wei-Kai-Xin-San (JWKXS) is a Chinese medicine formula applied for treating morbid forgetfulness in ancient China. Today, this formula is frequently applied for Alzheimer’s disease and vascular dementia (VD) in clinic. Here, we developed it as granules and aimed to evaluate its anti-AD effect on β amyloid protein 1–42 (Aβ1-42) induced cognitive deficit mice and reveal the possible molecular mechanisms. Firstly, daily intra-gastric administration of chemically standardized of JWKXS granules for 7 days significantly ameliorated the cognitive deficit symptoms and inhibited cell apoptosis in hippocampus on Aβ1-42 injection mice. JWKXS granules significantly decreased Aβ level, increased superoxide dismutase activity and decreased malondialdehyde level in hippocampus of model mice. It also restored acetylcholine amounts, inhibited acetylcholinesterase activities and increased choline acetyltransferase activities. In addition, JWKXS granules enabled the transformation of precursors of NGF and BDNF into mature forms. Furthermore, JWKXS granules could regulate gene expressions related to Aβ production, transportation, degradation and neurotrophic factor transformation, which led to down-regulation of Aβ and up-regulation of NGF and BDNF. These findings suggested that JWKXS granules ameliorated cognitive deficit via decreasing Aβ levels, protecting neuron from oxidation damages and nourishing neuron, which could serve as alternative medicine for patients suffering from AD.

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Effects of vindoline on rat cytochrome P450 isoform activities in vitro

Zhang

Xie

Liu

et al. 2022

Biomedical Chromatography

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A specific ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) method has been described for the simultaneous determination of the metabolites of tacrine, bupropion, diclofenac, dextromethorphan and midazolam, which are the five probe drugs of the five cytochrome P450 (CYP450) isoforms CYP1A2, CYP2B, CYP2C11, CYP2D1 and CYP3A4.The inhibition degree was determined by calculating the IC 50 . The chromatographic separation was performed on a C 18 column with a mobile phase consisting of 0.1% formic acid and acetonitrile. The mass spectrometric analysis was conducted in positive electrospray ionization mode. The IC 50 values of CYP1A2, CYP2B, CYP2C11, CYP2D1 and CYP3A were 113.4, 83.78, 22.50, 9.081 and 52.76 μmol L À1 , respectively. The in vitro results demonstrated that vindoline could inhibit CYP2D1 activity in rats, and weak inhibitory effect on CYP2C11 and CYP3A, but had no obvious effects on CYP1A2 and CYP2B.

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A UHPLC-QTOF-MS/MS method for the simultaneous determination of eight triterpene compounds from Poria cocos (Schw.) Wolf extract in rat plasma: Application to a comparative pharmacokinetic study

Cited by 31 publications

References 30 publications

Poria Cocos Ameliorates Bone Loss in Ovariectomized Mice and Inhibits Osteoclastogenesis In Vitro

Poria Cocos Ameliorates Bone Loss in Ovariectomized Mice and Inhibits Osteoclastogenesis In Vitro

Jia-Wei-Kai-Xin-San, an Herbal Medicine Formula, Ameliorates Cognitive Deficits via Modulating Metabolism of Beta Amyloid Protein and Neurotrophic Factors in Hippocampus of Aβ1-42 Induced Cognitive Deficit Mice

Effects of vindoline on rat cytochrome P450 isoform activities in vitro

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