A Type of Urea-Splitting Bacterium Found in the Human Intestinal Tract

Cooke, Jean V.; Keith, Hazel Reed

doi:10.1128/jb.13.5.315-319.1927

Cited by 21 publications

(6 citation statements)

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“…Corynebacterium ammoniagenes is a species with standing in nomenclature. It was first described by Cooke and Keith (1927) as Brevibacterium ammoniagenes, a urea-splitting bacterium isolated from the human intestinal tract. It was transferred to the genus Corynebacterium by Collins (1987).…”

Section: Corynebacterium Ammoniagenesmentioning

confidence: 99%

Scientific Opinion on the update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA (2017–2019)

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2020

EFS2

243

207

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The qualified presumption of safety (QPS) was developed to provide a safety pre‐assessment within EFSA for microorganisms. Strains belonging to QPS taxonomic units (TUs) still require an assessment based on a specific data package, but QPS status facilitates fast track evaluation. QPS TUs are unambiguously defined biological agents assessed for the body of knowledge, their safety and their end use. Safety concerns are, where possible, to be confirmed at strain or product level, and reflected as ‘qualifications’. Qualifications need to be evaluated at strain level by the respective EFSA units. The lowest QPS TU is the species level for bacteria, yeasts and protists/algae, and the family for viruses. The QPS concept is also applicable to genetically modified microorganisms used for production purposes if the recipient strain qualifies for the QPS status, and if the genetic modification does not indicate a concern. Based on the actual body of knowledge and/or an ambiguous taxonomic position, the following TUs were excluded from the QPS assessment: filamentous fungi, oomycetes, streptomycetes, Enterococcus faecium, Escherichia coli and bacteriophages. The list of QPS‐recommended biological agents was reviewed and updated in the current opinion and therefore now becomes the valid list. For this update, reports on the safety of previously assessed microorganisms, including bacteria, yeasts and viruses (the latter only when used for plant protection purposes) were reviewed, following an Extensive Literature Search strategy. All TUs previously recommended for 2016 QPS list had their status reconfirmed as well as their qualifications. The TUs related to the new notifications received since the 2016 QPS opinion was periodically evaluated for QPS status in the Statements of the BIOHAZ Panel, and the QPS list was also periodically updated. In total, 14 new TUs received a QPS status between 2017 and 2019: three yeasts, eight bacteria and three algae/protists.

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Section: Corynebacterium Ammoniagenesmentioning

confidence: 99%

Scientific Opinion on the update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA (2017–2019)

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2020

EFS2

243

207

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“…Urease is an enzyme of wide distribution as evidenced by the fact that some 200 species of bacteria, as well as higher plants and animals have been found to possess it (Sumner and Somers, 1953). Pasteur and Miguel were reported to have isolated "urobacilli" from air, soil, and sewage (Cooke and Keith, 1927). Subsequently, the enzyme was found in many soil and intestinal bacteria (Gibson, 1934;Stuart et al, 1945); but it is conspicuously absent among obligately anaerobic organisms.…”

mentioning

confidence: 99%

Physiological Study of an Obligately Anaerobic Ureolytic Bacterium

Gibbons

Doetsch

1959

J Bacteriol

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“…Although we used IMP production as an indicator in this study, natural substrates of the transporter remain unknown. C. stationis was isolated from the intestinal tract of infants based on ammonia production from urea (Cooke and Keith 1927 ). The growth of animal fecal bacteria relies on vitamins and enzyme cofactors from other members of the gut microbiota (Das et al 2019 ; Sharma et al 2019 ; Soto-Martin et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Mutational analysis in Corynebacterium stationis MFS transporters for improving nucleotide bioproduction

Kinose,

Shinoda,

Konishi

et al. 2024

Appl Microbiol Biotechnol

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Product secretion from an engineered cell can be advantageous for microbial cell factories. Extensive work on nucleotide manufacturing, one of the most successful microbial fermentation processes, has enabled Corynebacterium stationis to transport nucleotides outside the cell by random mutagenesis; however, the underlying mechanism has not been elucidated, hindering its applications in transporter engineering. Herein, we report the nucleotide-exporting major facilitator superfamily (MFS) transporter from the C. stationis genome and its hyperactive mutation at the G64 residue. Structural estimation and molecular dynamics simulations suggested that the activity of this transporter improved via two mechanisms: (1) enhancing interactions between transmembrane helices through the conserved “RxxQG” motif along with substrate binding and (2) trapping substrate-interacting residue for easier release from the cavity. Our results provide novel insights into how MFS transporters change their conformation from inward- to outward-facing states upon substrate binding to facilitate efflux and can contribute to the development of rational design approaches for efflux improvements in microbial cell factories. Keypoints • An MFS transporter from C. stationis genome and its mutation at residue G64 were assessed • It enhanced the transporter activity by strengthening transmembrane helix interactions and trapped substrate-interacting residues • Our results contribute to rational design approach development for efflux improvement Graphical Abstract

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A Type of Urea-Splitting Bacterium Found in the Human Intestinal Tract

Cited by 21 publications

References 0 publications

Scientific Opinion on the update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA (2017–2019)

Scientific Opinion on the update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA (2017–2019)

Physiological Study of an Obligately Anaerobic Ureolytic Bacterium

Mutational analysis in Corynebacterium stationis MFS transporters for improving nucleotide bioproduction

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