A two year global evaluation of the susceptibility of Candida species to fluconazole by disk diffusion

Liebowitz, L D; Ashbee, H. R.; Evans, E. G. V.; Chong, Yunsop; Mallátová, Naďa; Zaidi, Mussaret B.; Gibbs, David L.

doi:10.1016/s0732-8893(01)00243-7

Cited by 142 publications

(72 citation statements)

References 24 publications

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“…Este método, sin embargo, se practica sólo en laboratorios especializados que cuentan con personal capacitado. Por ello, se ha desarrollado un método más sencillo, como es el método de difusión de disco (20), el que, a su vez, ha sido modificado para lectura en un sistema computarizado (13,16,21) que lo hace más preciso y reproducible.…”

Section: Discussionunclassified

“…A pesar de que los aislamientos correspondientes a las especies más resistentes al fluconazol, como son C. krusei y C. glabrata, fueron relativamente infrecuentes, 2,2% y 3,5%, respectivamente, existe el peligro de que aumenten en el futuro, lo que señala la necesidad de estudios periódicos de vigilancia. Liebowitz et al (16) mostraron una disminución significativa (p>0,0001) de C. albicans al comparar dos años de estudio, 69,9% en 1998 y 67,3% en 1999, con el incremento subsiguiente de las especies diferentes a C. albicans.…”

Section: Discussionunclassified

“…Igual sucedió con otras especies que, aunque menos frecuentes, presentaron una baja cobertura en relación con la CIM 90. Los hallazgos anteriores están de acuerdo con lo informado en la literatura (7,8,(14)(15)(16)(17)22,24).…”

Section: Discussionunclassified

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Evaluación de la susceptibilidad de especies de Candida al fluconazol por el método de difusión de disco.

et al. 2003

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Las infecciones causadas por levaduras del género Candida han aumentado en forma dramática en las últimas décadas, especialmente a nivel hospitalario. Paralelamente con este aumento, se ha notado la aparición de resistencia a los antimicóticos, así como la selección de especies diferentes a Candida albicans, todo lo cual ha hecho necesaria la estandarización de pruebas de susceptibilidad in vitro. Entre las varias pruebas disponibles está la de difusión en agar con discos impregnados de antifúngicos, cuya lectura se hace electrónicamente (BIOMIC), lo que proporciona indicaciones objetivas de la concentración inhibitoria mínima (CIM). Se realizó el estudio para determinar la sensibilidad in vitro al fluconazol de 2.139 aislamientos de especies de Candida provenientes de pacientes atendidos en consulta externa o en salas de hospitalización de centros asistenciales en Colombia, Ecuador y Venezuela (región CELA). Candida albicans fue el microorganismo más frecuentemente aislado (62%), seguido a distancia por Candida parapsilosis (11%), Candida tropicalis (8,5 %), Candida glabrata (3,5%) y Candida krusei (2,2%). La CIM demostró que el 88,1% de estos aislamientos eran susceptibles al fluconazol, 5,1% eran susceptibles-dosisdependiente y 6,8% resistentes. El 92,1% de la especie C. albicans fue susceptible mientras que la resistencia al fluconazol fue más notoria en las restantes especies. El BIOMIC es un método rápido y sencillo para la vigilancia epidemiológica de resistencia en levaduras del género Candida recuperadas de pacientes atendidos en centros hospitalarios.Palabras clave: candidiasis, Candida, resistencia anfúngicos, fluconazol, prueba de disco. Resistance to fluconazole by Candida species detected using the disk diffusion method.Infections caused by yeasts belonging to the genus Candida have increased dramatically in the last decades, especially in hospital settings. Concomittantly, antimycotic resistance has emerged, as well as the appearance of non-Candida albicans isolates. To standardize in vitro antifungal susceptibility tests, the agar diffusion test was developed using disks impregnated with the antimycotic compound. Electronic recording of the inhibition zone (BIOMIC), furnishes objective values for the minimal inhibitory concentration (MIC). The fluconazole susceptibility patterns were determined for Candida species isolated from 2.139 patients seen in outpatient clinics or in health-care centers in Colombia, Ecuador and Venezuela. Candida albicans was the species most frequently isolated (62%), followed at a distance by Candida parapsilosis (11%), Candida tropicalis (8.5%), Candida glabata (3.5% ) and Candida krusei (2.2%). MIC determinations showed that 88.1% of these isolates were susceptible to fluconazole, 5.1% were susceptible-dose-dependant and 6.8% resistant. An important proportion (92.1%) of the C. albicans isolates proved susceptible while resistance predominated in the remaining species. These results indicate that the BIOMIC method is rapid and simple, constituting a suitable tool for ...

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Evaluación de la susceptibilidad de especies de Candida al fluconazol por el método de difusión de disco.

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“…An approved standard method (M38-A) disc diffusion method which had been applied to filamentous fungi tested [24] [25] [26] to evaluate the pathological activity of filamentous fungi to antifungal agents. Using an approved standard method (M44-P) had been used for disc diffusion method for yeasts developed by [25].…”

Section: Biologymentioning

confidence: 99%

“…Good alternatives methods had been characterized such as E-test and disk diffusion and they were simpler and faster techniques [26].…”

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Green Synthesis of Novel 5-Arylazo-2-[(2S, 3S, 4R, 5R)-3, 4, 5-Trihydroxy-6-(Hydroxymethyl) Tetrahydro-2H-Pyran-2-Yloxy]-4, 6-Dimethyl 3-Nicotinonitrile

Abdellattif¹,

Arief²,

Abdel‐Rahman³

et al. 2017

IJOC

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Introduction: Pyridone derivatives played important roles in the last decade to approach many and different functionalities, especially as antitumor, antibacterial, anti-fungal, and many of pharmacological activities. Methodology: Novel compounds of 5-Arylazo-2-[(substituted)-3, 4, 5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-2-yloxy]-4, 6-dimethylnicotinonitrile, (3a-e), generally called (fluroarylazopyridine glucosides) were synthesised via green protocol, microwave. Results: The compounds were investigated by (IR, 1 HNMR, 13 CNMR and mass spectrometry).Where some of pharmacological activities like antibacterial and antifungal studies had been investigated and characterized. It was found that 3a-d had characterized by high activities as antibacterial and antifungal. Where microwave synthetic methods were more efficient, gave higher products quantity, and more saving for time requirement and for using of much more solvents.

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Characterization and thermal studies of nano‐synthesized Mn(II), Co(II), Ni(II) and Cu(II) complexes with adipohydrazone ligand as new promising antimicrobial and antitumor agents

Gaber

Khedr

Elsharkawy

2017

Applied Organom Chemis

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New seven complexes of N 1 ,N 6 -bis((2-hydroxynaphthalin-1-yl)methinyl)) adipohydrazone (H 2 L) with MnCl 2 •4H 2 O, CoCl 2 •6H 2 O, NiCl 2 •6H 2 O, CuCl 2 •2H 2 O, Cu(NO 3 ) 2 •3H 2 O, CuSO 4 •5H 2 O, and Cu(OAc) 2 •2H 2 O have beenprepared and characterized by the aid of elemental and thermal analyses, spectra (FT-IR, 1 H NMR, MS, UV-Vis, ESR, X-ray powder diffraction), molar conductance and magnetic moment measurements. The XRD results unambiguously confirmed the nano-sized particles of the complexes. The results showed that H 2 L behaves as dibasic tetra-dentate ligand towards the metal ions of interest. The low molar conductance values revealed the non-electrolytic nature for the chelates. The magnetic moment data, UV-Vis and ESR spectra denoted the formation of octahedral geometries for Mn(II) and Ni(II) complexes, whereas Co(II), Cu(II) complexes exhibited tetrahedral arrangement. The activation parameters for the thermal decomposition stages were calculated from TGA curves using Coats-Redfern and Horowitz-Metzger methods. The obtained data were confirmed by 3-D molecular modeling of the ligand and some complexes. The investigated compounds were screened for their antimicrobial activities against different types of organisms and antitumor activities towards human liver Carcinoma (HEPG2) cell to access their potential chemotherapeutic use. The free ligand (H 2 L) exhibited a weak inhibition of cell viability with IC 50 of 11.80 μg/ml, complexes 4, 6 and 7 showed a moderate activity with IC 50 of 5.56, 7.71 and 5.67 μg/ml, whereas complexes 1, 2, 3, and 5 displayed a strong anticancer activity with IC 50 of 4.65, 3.97, 3.30 and 4.84 μg/ml, compared with IC 50 value of 4.73 μg/ml for the doxorubicin (standard cytotoxin drug).

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A two year global evaluation of the susceptibility of Candida species to fluconazole by disk diffusion

Cited by 142 publications

References 24 publications

Evaluación de la susceptibilidad de especies de Candida al fluconazol por el método de difusión de disco.

Evaluación de la susceptibilidad de especies de Candida al fluconazol por el método de difusión de disco.

Green Synthesis of Novel 5-Arylazo-2-[(2S, 3S, 4R, 5R)-3, 4, 5-Trihydroxy-6-(Hydroxymethyl) Tetrahydro-2H-Pyran-2-Yloxy]-4, 6-Dimethyl 3-Nicotinonitrile

Characterization and thermal studies of nano‐synthesized Mn(II), Co(II), Ni(II) and Cu(II) complexes with adipohydrazone ligand as new promising antimicrobial and antitumor agents

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