A two step process for production of omega 3-polyunsaturated fatty acid concentrates from sardine oil using Cryptococcus sp. MTCC 5455 lipase

Aarthy, Mayilvahanan; Palanivel, Saravanan; Ayyadurai, Niraikulam; Gowthaman, M.K.; Kamini, Numbi Ramudu

doi:10.1016/j.molcatb.2015.12.013

Cited by 25 publications

(13 citation statements)

References 38 publications

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“…Another commercial lipase, porcine pancreatic lipase (Type 2), was also inefficient for the hydrolysis of soybean oil because a hydrolysis yield of less than 25% was obtained at a reaction time of 24 h . Commercial lipases from Candida rugosa (Type VII), Cryptococcus sp., and Lipozyme TL 100 L displayed high catalytic efficiency with hydrolysis yields of 70%, 83.7%, and 86%, respectively, after 24 h . To enhance the hydrolysis efficiency, Ting et al immobilized lipase derived from C. rugosa to chitosan beads and used the enzyme for soybean oil hydrolysis.…”

Section: Resultsmentioning

confidence: 99%

“…The sample was then titrated against 0.1 M KOH using phenolphthalein as the indicator. The hydrolysis yield was calculated on the basis of the acid value (AV) and saponification value (SV) as follows

Hydrolysis yield (() %) = \frac{AV}{SV} \times 100 = \frac{M_{KOH} \times C_{KOH} \times V_{KOH}}{SV \times m} \times 100

where M KOH is the molecular weight of KOH, C KOH is the molar concentration of KOH, V KOH is the volume of KOH used in the titration procedure (mL), and m is the sample weight (g). The saponification value of the gac oil was 192.41 mg KOH/g.…”

Section: Methodsmentioning

confidence: 99%

Section: Determination Of the Hydrolysis Yieldmentioning

confidence: 99%

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Liquid lipase‐catalyzed hydrolysis of gac oil for fatty acid production: Optimization using response surface methodology

Nguyen

et al. 2018

Biotechnology Progress

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Fatty acids are valuable products because they have wide industrial applications in the manufacture of detergents, cosmetics, food, and various biomedical applications. In enzyme‐catalyzed hydrolysis, the use of immobilized lipase results in high production cost. To address this problem, Eversa Transform lipase, a new and low‐cost liquid lipase formulation, was used for the first time in oil hydrolysis with gac oil as a triglyceride source in this study. Response surface methodology was employed to optimize the reaction conditions and establish a reliable mathematical model for predicting hydrolysis yield. A maximal yield of 94.16% was obtained at a water‐to‐oil molar ratio of 12.79:1, reaction temperature of 38.9 °C, enzyme loading of 13.88%, and reaction time of 8.41 h. Under this optimal reaction condition, Eversa Transform lipase could be reused for up to eight cycles without significant loss in enzyme activity. This study indicates that the use of liquid Eversa Transform lipase in enzyme‐catalyzed oil hydrolysis could be a promising and cheap method of fatty acid production. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 2018

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Section: Resultsmentioning

confidence: 99%

Hydrolysis yield (() %) = \frac{AV}{SV} \times 100 = \frac{M_{KOH} \times C_{KOH} \times V_{KOH}}{SV \times m} \times 100

Section: Methodsmentioning

confidence: 99%

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Liquid lipase‐catalyzed hydrolysis of gac oil for fatty acid production: Optimization using response surface methodology

Nguyen

et al. 2018

Biotechnology Progress

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“…The extracellular crude lipase obtained from Cryptococcus sp . was partially purified by ammonium sulfate precipitation (80% saturation).…”

Section: Methodsmentioning

confidence: 99%

“…Among them, the lipase from Cryptococcus sp. MTCC 5455 is unique and has potential in synthesis of alkyl esters, removal of triglyceride soil from fabrics, hydrolysis of oils, and degradation of aliphatic polymers in aqueous conditions . Therefore, an attempt was made for the first time to synthesize an industrially important fatty acid ester, starch oleate using the native lipase from Cryptococcus sp.…”

Section: Introductionmentioning

confidence: 99%

A Green Process for Starch Oleate Synthesis by Cryptococcus sp. MTCC 5455 Lipase and Its Potential as an Emulsifying Agent

et al. 2018

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Starch oleate is synthesized in an aqueous medium using lipase from the yeast Cryptococcus sp. MTCC 5455. The optimum conditions of esterification are found at 24 h and 30 °C with an oleic acid/starch molar ratio of 1:2 using 500 U of lipase and the degree of substitution was 0.26. Spectral techniques confirm the presence of oleate group in the modified potato starch. Scanning electron microscopic and X‐ray diffraction studies also reveal the morphological and crystallographic properties of starch which are disrupted during the esterification process. Thermogravimetric analysis indicates the decrease in thermal stability of starch oleate due to the transformed structure of starch from semi crystalline to an amorphous form. The synthesized starch oleate could impart 85% stability to emulsions and has potential as an emulsifier in food sector owing to its eco‐friendly preparation.

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Mass Transfer Modeling of α‐Eleostearic Acid from Tung Oil Concentration by Low‐Temperature Crystallization

et al. 2020

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With the significant attention paid to α-eleostearic acid (α-ESA) in a variety of biologically beneficial effects, the demand for high-concentration products is increasing. A method of cryogenic separation to predict the mass variation of α-ESA from tung oil was established in this study. The results showed that the best α-ESA crystallization condition was À 20°C in ethanol, which implied a low energy consumption when compared with the common crystallization temperature of À 60°C. The α-ESA concentration in the liquid phase was reduced by 90.4% compared to tung oil, and most of the α-ESA in the solution was recovered by mass transfer. On the other hand, a mathematical model based on the diffusion reaction theory is used in mass variation prediction. The effects of temperature and time on the mass variation in α-ESA in solution crystallization were examined. The results showed that the proposed model could estimate the composition and total mass of winterized tung oil at R 2 = 0.974. The model was validated by experiments with an error of � 10%.[a] J.

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A two step process for production of omega 3-polyunsaturated fatty acid concentrates from sardine oil using Cryptococcus sp. MTCC 5455 lipase

Cited by 25 publications

References 38 publications

Liquid lipase‐catalyzed hydrolysis of gac oil for fatty acid production: Optimization using response surface methodology

Liquid lipase‐catalyzed hydrolysis of gac oil for fatty acid production: Optimization using response surface methodology

A Green Process for Starch Oleate Synthesis by Cryptococcus sp. MTCC 5455 Lipase and Its Potential as an Emulsifying Agent

Mass Transfer Modeling of α‐Eleostearic Acid from Tung Oil Concentration by Low‐Temperature Crystallization

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