A two-step homogeneous assay for apolipoprotein E-containing high-density lipoprotein-cholesterol

Takahashi, Yuji; Ito, Yasuki; Sakurai, ﻿Toshihiro; Wada, Norio; Nagasaka, Atsushi; Fujikawa, Masato; Chiba, Hitoshi; Hui, Shu‐Ping

doi:10.1177/0004563218795212

Cited by 3 publications

(2 citation statements)

References 33 publications

(59 reference statements)

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“…Serum creatinine, liver transaminases, TC, TG, HDL-C, HDL3-C, apoE-HDL-C, direct LDL-C, sdLDL-C, LDL-TG, and RLP-C were measured by automated standardized enzymatic analysis on a Cobas C501 analyzer (Roche Diagnostics, Germany). The specialized lipid assay kits were provided by the Denka-Seiken Corporation (Tokyo, Japan) as previously described 24, 31, 32, 38–43) . All specialized lipid assays had within and between run CVs were < 6.0%, and in most cases < 3.0%.…”

Section: Methodsmentioning

confidence: 99%

Lipoprotein Subfractions and Glucose Homeostasis in Prediabetes and Diabetes in Taiwan

Hsu

Cheng

et al. 2019

JAT

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Aims: Prediabetes and diabetes are associated with increased insulin resistance and decreased insulin production, dyslipidemia, and increased cardiovascular disease (CVD) risk. Our goals were to assess lipoprotein subfractions using novel assays in such subjects.Methods: Fasting normal, prediabetic, and diabetic Taiwanese men and women (n = 2,049) had their serum glucose, glycosylated hemoglobin, insulin, total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), HDL3-C, apolipoprotein E-HDL-C, direct low-density lipoprotein cholesterol (LDL-C), small dense LDL-C (sdLDL-C), LDL-TG, and remnant lipoprotein cholesterol (RLP-C) levels measured using novel assays. HDL2-C, LDL-C, and large-buoyant LDL-C (lbLDL-C) were calculated.Results: Prediabetic male and female subjects had significantly higher levels of TG, RLP-C, sdLDL-C, the sdLDL-C/LDL-C ratio, and LDL-TG than normal subjects, and statin treatment abolished this effect in men, but not in women. Diabetic male and female subjects had significantly higher TG and sdLDL-C/LDL-C ratios, and significantly lower levels of HDL-C, HDL2-C, HDL3-C, and apoE HDL-C than normal subjects, as did prediabetic women. Median direct LDL-C levels were > 100 mg/dL in all groups, even in those receiving statin therapy. Calculated LDL-C significantly underestimated direct LDL-C by > 10% in diabetic subjects.Conclusions: Our data indicate that prediabetic subjects were more likely to have significantly elevated RLP-C, sdLDL-C, and LDL-TG, while diabetic subjects were more likely to have significantly decreased HDL-C, HDL2-C, HDL3-C, and apoE HDL-C than normal subjects, and calculated LDL-C significantly underestimated their direct LDL-C. In our view, direct LDL-C and sdLDL-C should be measured and optimized in both diabetic and prediabetic subjects to reduce CVD risk.

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Section: Methodsmentioning

confidence: 99%

Lipoprotein Subfractions and Glucose Homeostasis in Prediabetes and Diabetes in Taiwan

Hsu

Cheng

et al. 2019

JAT

Self Cite

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“…The incremental area under the curve (iAUC) was calculated by subtracting fasting AUC from total AUC. sdLDL-C 5) , LDL-TG 9) , HDL3-C 20) , apoE-rich HDL-C 21) , and remnant-like particle (RLP)-C 22) were measured by the homogeneous method (Denka Co., Ltd. Tokyo, Japan). TRL-C was measured directly by the homogeneous method (Denka Co., Ltd.) using a twostep process where LDL and HDL are degraded, and lipoprotein spectrums from TG-rich lipoproteins (TRLs) to HDL 3) .…”

Section: Methodsmentioning

confidence: 99%

Circadian Rhythm of Subspecies of Low-Density Lipoprotein-Cholesterol and High-Density Lipoprotein-Cholesterol in Healthy Subjects and Patients with Type 2 Diabetes

Hayashi

Goto

et al. 2023

JAT

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Aims:We established automated assay kits for quantifying small dense low-density lipoprotein (sdLDL)cholesterol (C), LDL-triglyceride (TG), and high-density lipoprotein (HDL)3-C, and apolipoprotein (apo)E-rich HDL-C, and these have been recognized as sensitive biomarkers for predicting coronary artery disease. We investigated the circadian rhythms of these novel lipids to determine if fasting is required to determine basal levels. Methods: Forty-eight inpatients with type 2 diabetes and 19 healthy volunteers were studied. Blood samples were collected at seven time points, which were obtained after an overnight fast, before and 2 h after each meal, and before the next breakfast. sdLDL-C, LDL-TG, remnant-like particle (RLP)-C, TG-rich lipoprotein (TRL-C), HDL3-C, and apoE-rich HDL-C were measured by the homogeneous methods. NonHDL-C, large buoyant (lb) LDL-C and HDL2-C were calculated by subtracting sdLDL-C from LDL-C or HDL3-C from HDL-C, respectively.Results: Serum TG levels were significantly increased after meals in both healthy participants and patients with diabetes. RLP-C and TRL-C were also increased postprandially. LDL-TG, LDL-C, nonHDL-C, HDL2,3-C, and apoE-rich HDL-C did not exhibit significant fluctuation during the day in healthy participants and patients with diabetes. sdLDL-C was slightly increased postprandially in subjects with diabetes (1-2 mg/dl, 3%-9%), though its increase was not significant compared to the baseline (fasting) level. Significant postprandial reduction was observed with LDL-C and lbLDL-C. There was no influence of statin therapy or oral anti-diabetes drugs on the circadian rhythm of LDL-C subspecies.Conclusions: Subtle postprandial increase in sdLDL-C is considered a negligible level in general clinical practice. Fasting is not mandatory to measure basal concentrations of LDL and HDL subspecies.

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Evaluation of the apolipoprotein E (apoE)-HDL-associated risk factors for coronary heart disease using duo-functional electrochemical aptasensor

Tsai

Huang

et al. 2022

Anal Bioanal Chem

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A two-step homogeneous assay for apolipoprotein E-containing high-density lipoprotein-cholesterol

Cited by 3 publications

References 33 publications

Lipoprotein Subfractions and Glucose Homeostasis in Prediabetes and Diabetes in Taiwan

Lipoprotein Subfractions and Glucose Homeostasis in Prediabetes and Diabetes in Taiwan

Circadian Rhythm of Subspecies of Low-Density Lipoprotein-Cholesterol and High-Density Lipoprotein-Cholesterol in Healthy Subjects and Patients with Type 2 Diabetes

Evaluation of the apolipoprotein E (apoE)-HDL-associated risk factors for coronary heart disease using duo-functional electrochemical aptasensor

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