A Two-stage Process for Increasing the Yield of Prebiotic-rich Extract from Pinus densiflora

Abstract

The importance of polysaccharides is increasing globally due to their role as a significant source of dietary prebiotics in the human diet. In the present study, in order to maximize the yield of crude polysaccharides from Pinus densiflora, response surface methodology (RSM) was used to optimize a two-stage extraction process consisting of steam explosion and water extraction. Three independent main variables, namely, the severity factor (Ro) for the steam explosion process, the water extraction temperature (°C), and the ratio of water to raw material (v/w), were studied with respect to prebiotic sugar content. A Box-Behnken design was created on the basis of the results of these single-factor tests. The experimental data were fitted to a second-order polynomial equation for multiple regression analysis and examined using the appropriate statistical methods. The data showed that both the severity factor (Ro) and the ratio of water to material (v/w) had significant effects on the prebiotic sugar content. The optimal conditions for the two-stage process were as follows: a severity factor (Ro) of 3.86, a water extraction temperature of 89.66 °C, and a ratio of water to material (v/w) of 39.20. Under these conditions, the prebiotic sugar content in the extract was 332.45 mg/g.

Evaluation of the Basic Properties for the Korean Major Domestic Wood Species

“…As a result, vegetative structures are relatively complex for the territorial area, with a diversity of tree species. In the Korean Plant Names Index (KPNI), the processes and the characteristics of the material (Choi et al, 2020Gong et al, 2021;Han et al, 2022;Jang, 2022c;Jung and Yang, 2018;Jung et al, 2019Jung et al, , 2021Jung et al, , 2022Kim, 2018, 2021;Kim et al, 2017Kim et al, , 2020Lee and Bae, 2021;Lee and Kim, 2022;Lee et al, 2021cLee et al, , 2022aLee et al, , 2022bLee et al, , 2022cMin et al, 2019).…”

Natural Dye Extraction from Merbau (Intsia bijuga) Sawdust: Optimization of Solid–Solvent Ratio and Temperature

“…They identified the optimal extraction conditions using response surface methodology (RSM; Jung et al, 2017). Jung and Yang (2018) employed RSM to optimize a two-stage extraction process that consisted of steam explosion and water extraction (Jung and Yang, 2018). The study conducted by Um et al (2020) aimed to investigate the optimization of ascorbic acid extraction from Rugosa rose (Rosa rugosa Thunb.)…”