A tunable Caco-2/HT29-MTX co-culture model mimicking variable permeabilities of the human intestine obtained by an original seeding procedure

Biorelevant media resistant co-culture model mimicking permeability of human intestine

“…Intestinal absorption of digoxin in TB across the co-culture was intermediary between Caco-2 monolayers and HT29-MTX single cultures. This result was consistent with transport studies performed with rhodamine 123 in a previous work, which showed that P-gp expression was lower in the co-culture model compared to Caco-2 monolayers (Béduneau et al, 2014). Both FaSSIF and FeSSIF induced a decrease of the secretory transport of both rhodamine 123 and digoxin.…”

“…Moreover, intestinal absorption of propranolol in TB was similar between co-culture and HT29-MTX single culture whereas the apparent permeability across the Caco-2 monolayer was significantly higher. This was consistent with a previous study which demonstrated that goblet cells were predominant in the co-culture (Béduneau et al, 2014). Interestingly, control consisting in the addition of FeSSIF both in apical and basolateral compartments of the co-culture induced a dramatic toxic effect.…”

“…Associations of different cell types in one cell lines were suggested to turn these models more realistic and come closer to the barrier properties of the human small intestine (Antunes et al, 2013;Lasa-Saracíbar et al, 2014). The combination of Caco-2 and HT29-MTX mucin-secreting cells in one co-culture model has been reported, elucidating that the confluent cells were covered by a mucin layer and exhibited tailor-made barrier properties regarding the P-gp expression and paracellular permeability (Béduneau et al, 2014;Calatayud et al, 2012;Chen et al, 2010).…”

Flavonoid interactions during digestion, absorption, distribution and metabolism: a sequential structure–activity/property relationship-based approach in the study of bioavailability and bioactivity

“…This may explain why there seems to be a higher absorption of flavonoid glucosides in vivo than in vitro, considering that current cell-based models (usually Caco-2 cells) do not produce a mucus layer. Currently, cellular models involving co-culture of enterocytes with mucusproducing cells are increasingly being used to overcome this limitation, although data are scarce (Béduneau et al, 2014).…”

Drug Release and Targeting: the Versatility of Polymethacrylate Nanoparticles for Peroral Administration Revealed by Using an Optimized In Vitro-Toolbox

“…A tight cell layer is formed, producing a thin layer of mucin (46) with a composition closely related to the one seen in the human intestine (47). In contrast to co-cultures, e.g., of Caco-2 and HT29 cells (48), which provide deeper insights into the mechanisms of colloid-surface interaction at the human gut wall, culturing techniques for T-84 cells are relatively simple assuring shorter preparation times and a standardized measurement.…”