A tumor cell membrane-coated self-amplified nanosystem as a nanovaccine to boost the therapeutic effect of anti-PD-L1 antibody

Lactose-modified enzyme-sensitive branched polymers as a nanoscale liver cancer-targeting MRI contrast agent

“…The mice were sacrificed at 0.5 h, 1 h, 2 h and 24 h after injection, and their hearts, livers, spleens, lungs and kidneys and tumors were collected for fluorescence imaging (IVIS spectrum). 34…”

Konjac glucomannan hydrogel dressing and its combination with Chinese medicine for the wound treatment

“…Cell compatibility was tested using an MTT assay and live/dead staining was performed. 41 First, the KGM hydrogel was sterilized by ultraviolet light irradiation. L929 mouse fibroblast cells were treated with a cut 0.01 g weight of konjac glucomannan hydrogel and incubated for 24 and 72 h. The medium was discarded and cultured with MTT containing serum-free cell culture medium for 4 h. The MTT was then removed and DMSO was added to read the absorbance at 490 nm and the cell viability was calculated according to a previous study.…”

Nanomedicine embraces cancer radio-immunotherapy: mechanism, design, recent advances, and clinical translation

“…Biomimetic nanocarriers have the reputation for their biocompatibility, the intrinsic tumor-homing effect, and inherent immunogenic properties. 185–187 A myriad of engineered biomimetic membrane-based vesicles (single membrane-based vesicles, fused cell membrane-based vesicles, and exosomes), originating from blood cells, platelets, tumor cells, immune cells, and bacteria, have been utilized to deliver antigenic agents or other therapeutic agents to elicit an immune response in the practice of cancer vaccination or radio-immunotherapy. 188–192 These biomimetic nanocarriers provide a strong shelter to prevent premature leakage and enzymatic lysis of cargos such as nucleic acids or proteins.…”