A true theranostic approach to medicine: Towards tandem sensor detection and removal of endotoxin in blood

Thompson, Michael; Blaszykowski, Christophe; Sheikh, Sonia; Romaschin, Alexander D.

doi:10.1016/j.bios.2014.07.008

Cited by 26 publications

(29 citation statements)

References 44 publications

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“…However, a growing body of BAW sensors presented in the literature outline instances, where the observed sensor response arises from the structural rearrangement of biomolecular probes immobilized on the sensing surface (Feiler et al, 2007;Lee et al, 2010;Thompson et al, 2015;Wang et al, 2006). In the case in point, we postulate that the signal observed for the M4CA platform is due to a combination of both the added mass of the cocaine analyte coupled to the tertiary structure rearrangement of MN4 aptamer upon cocaine binding.…”

Section: Sensor Performancementioning

confidence: 92%

“…The coil and piezoelectric substrate are located in a sample cell, wherein a running buffer is flowed, hence the EMPAS operates in an online and real-time detection format. In practice, the sensor has been successfully applied for the detection of proteins (Sheikh et al, 2011(Sheikh et al, , 2010, and lipopolysaccharide pathogens (Thompson et al, 2015), as well as for the evaluation of surface fouling (Sheikh et al, 2013. Functionalization of the EMPAS sensor platforms is generally achieved through the use of conditioning organic adlayers.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ultra-high frequency piezoelectric aptasensor for the label-free detection of cocaine

Neves

Blaszykowski²,

Bokhari

et al. 2015

Biosensors and Bioelectronics

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Section: Sensor Performancementioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Ultra-high frequency piezoelectric aptasensor for the label-free detection of cocaine

Neves

Blaszykowski²,

Bokhari

et al. 2015

Biosensors and Bioelectronics

Self Cite

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“…In some cases, diagnostics/sensing and therapeutic properties have been combined onto single NPs, providing for innovative tools – Nanotheranostics. Recently, several nanotheranostics strategies against bacteria have been described (Kuo et al, 2009 ; DeGrasse, 2012 ; Dai X. et al, 2013 ; Khlebtsov et al, 2013 ; Kim et al, 2013 ; Gamella et al, 2014 ; Pei et al, 2014 ; Setyawati et al, 2014 ; Patel and Janjic, 2015 ; Thompson et al, 2015 ; Jagtap et al, 2017 ; Mocan et al, 2017 ; Zhao et al, 2017 ).…”

Section: Other Potential Applications Of Npsmentioning

confidence: 99%

Nano-Strategies to Fight Multidrug Resistant Bacteria—“A Battle of the Titans”

McCusker²,

et al. 2018

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Infectious diseases remain one of the leading causes of morbidity and mortality worldwide. The WHO and CDC have expressed serious concern regarding the continued increase in the development of multidrug resistance among bacteria. Therefore, the antibiotic resistance crisis is one of the most pressing issues in global public health. Associated with the rise in antibiotic resistance is the lack of new antimicrobials. This has triggered initiatives worldwide to develop novel and more effective antimicrobial compounds as well as to develop novel delivery and targeting strategies. Bacteria have developed many ways by which they become resistant to antimicrobials. Among those are enzyme inactivation, decreased cell permeability, target protection, target overproduction, altered target site/enzyme, increased efflux due to over-expression of efflux pumps, among others. Other more complex phenotypes, such as biofilm formation and quorum sensing do not appear as a result of the exposure of bacteria to antibiotics although, it is known that biofilm formation can be induced by antibiotics. These phenotypes are related to tolerance to antibiotics in bacteria. Different strategies, such as the use of nanostructured materials, are being developed to overcome these and other types of resistance. Nanostructured materials can be used to convey antimicrobials, to assist in the delivery of novel drugs or ultimately, possess antimicrobial activity by themselves. Additionally, nanoparticles (e.g., metallic, organic, carbon nanotubes, etc.) may circumvent drug resistance mechanisms in bacteria and, associated with their antimicrobial potential, inhibit biofilm formation or other important processes. Other strategies, including the combined use of plant-based antimicrobials and nanoparticles to overcome toxicity issues, are also being investigated. Coupling nanoparticles and natural-based antimicrobials (or other repurposed compounds) to inhibit the activity of bacterial efflux pumps; formation of biofilms; interference of quorum sensing; and possibly plasmid curing, are just some of the strategies to combat multidrug resistant bacteria. However, the use of nanoparticles still presents a challenge to therapy and much more research is needed in order to overcome this. In this review, we will summarize the current research on nanoparticles and other nanomaterials and how these are or can be applied in the future to fight multidrug resistant bacteria.

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“…There are several methods in the literature of the past few years suggesting that the future of these assays could depend on biosensors. For instance, protein-based [65-69], peptide-based [70-74], antibody-based [75-78], aptamer-based [11,79-83], cell-based [64,84-87], and other material-based [88-91] biosensors and assays have emerged. The notable trend of emerging LPS detection techniques is in association with the quest for simplicity and stability [92,93]; sensitivity and ease of handling [20]; reproducibility and reliability [94]; and, finally, robustness and cost efficiency [42].…”

Section: Endotoxin Detection and Quantificationmentioning

confidence: 99%

Elucidating endotoxin-biomolecule interactions with FRET: extending the frontiers of their supramolecular complexation

et al. 2017

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Endotoxin has been one of the topical chemical contaminants of major concern to researchers, especially in the field of bioprocessing. This major concern of researchers stems from the fact that the presence of Gram-negative bacterial endotoxin in intracellular products is unavoidable and requires complex downstream purification steps. For instance, endotoxin interacts with recombinant proteins, peptides, antibodies and aptamers and these interactions have formed the foundation for most biosensors for endotoxin detection. It has become imperative for researchers to engineer reliable means/techniques to detect, separate and remove endotoxin, without compromising the quality and quantity of the end-product. However, the underlying mechanism involved during endotoxin-biomolecule interaction is still a gray area. The use of quantitative molecular microscopy that provides high resolution of biomolecules is highly promising, hence, may lead to the development of improved endotoxin detection strategies in biomolecule preparation. Förster resonance energy transfer (FRET) spectroscopy is one of the emerging most powerful tools compatible with most super-resolution techniques for the analysis of molecular interactions. However, the scope of FRET has not been well-exploited in the analysis of endotoxin-biomolecule interaction. This article reviews endotoxin, its pathophysiological consequences and the interaction with biomolecules. Herein, we outline the common potential ways of using FRET to extend the current understanding of endotoxin-biomolecule interaction with the inference that a detailed understanding of the interaction is a prerequisite for the design of strategies for endotoxin identification and removal from protein milieus.

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A true theranostic approach to medicine: Towards tandem sensor detection and removal of endotoxin in blood

Cited by 26 publications

References 44 publications

Ultra-high frequency piezoelectric aptasensor for the label-free detection of cocaine

Ultra-high frequency piezoelectric aptasensor for the label-free detection of cocaine

Nano-Strategies to Fight Multidrug Resistant Bacteria—“A Battle of the Titans”

Elucidating endotoxin-biomolecule interactions with FRET: extending the frontiers of their supramolecular complexation

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