Ultra-high frequency piezoelectric aptasensor for the label-free detection of cocaine

Neves, Miguel A. D.; Blaszykowski, Christophe; Bokhari, Sumra; Thompson, Michael

doi:10.1016/j.bios.2015.05.038

Cited by 73 publications

(65 citation statements)

References 80 publications

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“…Biosensors have the great potential for rapid and on-site detection of analytes in body fluids and environment samples, due to ease for miniaturization and capability of measuring complex matrices with minimal sample preparation [7][8][9]. In the past few decades, biosensors have been developed to measure numerous analytes in various matrices, such as heavy metals [10,11], small molecule [12,13], targeted DNA [14], peptides [15], enzyme [16], protein [15,17], antigen [18], biomarkers [7,8] and even bacteria [19,20].…”

Section: Introductionmentioning

confidence: 99%

A novel colorimetric biosensor based on non-aggregated Au@Ag core–shell nanoparticles for methamphetamine and cocaine detection

Mao

Yang

et al. 2017

Talanta

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We report a novel colorimetric biosensor based on non-aggregation Au@Ag core-shell nanoparticles to detect methamphetamine and cocaine. The biosensor consisted of a reporter probe (RP) that is a specific single-stranded DNA (ssDNA) sequence coated on Au@Ag nanoparticles, a capture probe (CP) conjugated with magnetic beads, and an illicit drug-binding DNA aptamer (Apt). Au@Ag nanoparticles were synthesized by seed growth and characterized by scanning electron microscope (SEM), high-resolution transmission electron microscopy (HR-TEM), and UV-vis spectra. Methamphetamine (METH) was used as an example to evaluate the feasibility of the biosensor and to optimize the detection conditions. We demonstrated that this sensing platform was able to detect as low as 0.1nM (14.9ngL) METH with a negligible interference from other common illicit drugs. Various concentrations of METH were spiked into urines, and the biosensor yielded recoveries more than 83.1%. In addition, the biosensor also showed a high sensitivity to detect cocaine. These results demonstrated that our colorimetric sensor holds promise to be implemented as a visual sensing platform to detect multiple illicit drugs in biological samples and environmental matrices.

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Section: Introductionmentioning

confidence: 99%

A novel colorimetric biosensor based on non-aggregated Au@Ag core–shell nanoparticles for methamphetamine and cocaine detection

Mao

Yang

et al. 2017

Talanta

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“…In particular, DNA aptamers MN4 selective to the cocaine that were immobilized at the surface of the crystal by crosslinking with S-(11-trichlorosilyl-undecanyl)-benzenethiosulfonate. The detection of cocaine with immobilized aptamers by the EMPAS was possible with a limit of detection (LOD) of 0.9 μM [ 54 ]. Using the EMPAS and the crystal with immobilized antibodies against HIV, the label-free detection of HIV-1 and HIV-2 was performed.…”

Section: Surface Acoustic Methodsmentioning

confidence: 99%

Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods

Dizon

Tatarko

Hianik

2020

Sensors

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This review is focused on the application of surface and volume-sensitive acoustic methods for the detection of milk proteases such as trypsin and plasmin. While trypsin is an important protein of human milk, plasmin is a protease that plays an important role in the quality of bovine, sheep and goat milks. The increased activity of plasmin can cause an extensive cleavage of β-casein and, thus, affect the milk gelation and taste. The basic principles of surface-sensitive acoustic methods, as well as high-resolution ultrasonic spectroscopy (HR-US), are presented. The current state-of-the-art examples of the application of acoustic sensors for protease detection in real time are discussed. The application of the HR-US method for studying the kinetics of the enzyme reaction is demonstrated. The sensitivity of the acoustics biosensors and HR-US methods for protease detection are compared.

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“…Neves et al generated two sensitive cocaine aptasensors that rely on an electromagnetic piezoelectric acoustic sensor (EMPAS) platform as the basis of ultra-high frequency with tuned signal-to-noise ratio [53]. The sensing interface consists of a S-(11-trichlorosilyl-undecanyl) benzenethiosulfonate (BTS) adlayer-coated quartz disc onto which a structure-switching cocaine aptamer was immobilized, completing the preparation of the MN4 cocaine aptamer with an apparent Kd of 45 ± 12 μM and limit of detection of 0.9 μM.…”

Section: Acoustic Aptasensorsmentioning

confidence: 99%

Aptamers for Diagnostics with Applications for Infectious Diseases

İlgü¹,

Fazlioglu²,

Ozturk³

et al. 2019

Recent Advances in Analytical Chemistry

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Aptamers are in vitro selected oligonucleotides (DNA, RNA, oligos with modified nucleotides) that can have high affinity and specificity for a broad range of potential targets with high affinity and specificity. Here we focus on their applications as biosensors in the diagnostic field, although they can also be used as therapeutic agents. A small number of peptide aptamers have also been identified. In analytical settings, aptamers have the potential to extend the limit of current techniques as they offer many advantages over antibodies and can be used for real-time biomarker detection, cancer clinical testing, and detection of infectious microorganisms and viruses. Once optimized and validated, aptasensor technologies are expected to be highly beneficial to clinicians by providing a larger range and more rapid output of diagnostic readings than current technologies and support personalized medicine and faster implementation of optimal treatments.

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Ultra-high frequency piezoelectric aptasensor for the label-free detection of cocaine

Cited by 73 publications

References 80 publications

A novel colorimetric biosensor based on non-aggregated Au@Ag core–shell nanoparticles for methamphetamine and cocaine detection

A novel colorimetric biosensor based on non-aggregated Au@Ag core–shell nanoparticles for methamphetamine and cocaine detection

Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods

Aptamers for Diagnostics with Applications for Infectious Diseases

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