A Tricin Derivative from Deschampsia antarctica Desv. Inhibits Colorectal Carcinoma Growth and Liver Metastasis through the Induction of a Specific Immune Response

Abstract

In colorectal carcinoma patients, distant metastatic disease is present at initial diagnosis in nearly 25% of them. The majority of patients with metastatic colorectal carcinoma have incurable disease; therefore, new therapies are needed. Agents derived from medicinal plants have already demonstrated therapeutic activities in human cancer cells. Antartina is an antitumor agent isolated from Desv. This study aimed to evaluate the antitumor properties of Antartina in colorectal carcinoma models. We used human and murine colorectal carcinoma cell lines for investigating proliferation, apoptosis, and cell-cycle effects of Antartina therapy Avatar and immunocompetent colorectal carcinoma animal models were applied for evaluating the effects of Antartina Immune response against colorectal carcinoma model was investigated using CTL assay, analyzing dendritic cell activation and intratumor T-cell subpopulation, and by tumor rechallenge experiments. Antartina inhibits human colorectal carcinoma cell proliferation; however, experiments in Avatar colorectal carcinoma model Antartina display a limited antitumor effect. In an immunocompetent colorectal carcinoma mice model, Antartina potently inhibited tumor growth and liver metastases, leading to complete tumor regressions in>30% of mice and increased animal survival. In addition, Antartina induced a potent specific cytotoxic T-cell response against colorectal carcinoma and a long-lasting antitumor immunity. Interestingly, Antartina increased tumor immunogenicity and stimulated dendritic cell activation. No toxic effects were observed at the doses employed. Our findings showed that Antartina has the ability to induce antitumor immunity against colorectal carcinoma and can be used to develop new tools for the treatment of colorectal carcinoma. .

Preservation of Features of Anatomical Polymorphism of Deschampsia antarctica É. Desv. (Poaceae) During In Vitro Clonal Reproduction

“…Deschampsia antarctica is expected to be rich in molecules with anticancer activity (Silva et al, 2020). For example, a tricin derivative form D. antarctica inhibits the growth of colorectal cancer and liver metastasis (Malvicini et al, 2018). is usefulness is another important reason for studying these plants in vitro.…”

The Plastid Genome of Deschampsia cespitosa (Poaceae)

“…Other studies on D. antarctica have included the search of genes expressed under cold stress [17], the later development of cold resistant transgenic rice plants [18] and the resistance to oxidative stress after exposure to UVB radiation [19]. Recently, the effect of Antartina TM , which was developed from extracts of D. antarctica , has been evaluated as an inhibitor of colorectal carcinoma growth and liver metastasis [20].…”

Protection against UVB-Induced Wrinkle Formation in SKH-1 Hairless Mice: Efficacy of Tricin Isolated from Enzyme-Treated Zizania latifolia Extract

“…Tricin (4′,5,7-trihydroxy-3′,5′-dimethoxyflavone) identified from various plants is reported to inhibit the growth of colorectal carcinoma [ 1 ] and breast cancer cells [ 2 ] as well as influenza virus activity [ 3 ]. Several studies have demonstrated that tricin isolated from Njavara rice has anti-inflammatory and antioxidative properties [ 4 , 5 ]; tricin and its derivatives isolated from Zizania latifolia (ETZL) were previously identified as the active components for inhibition of allergies and inflammation [ 6 ].…”