Protection against UVB-Induced Wrinkle Formation in SKH-1 Hairless Mice: Efficacy of Tricin Isolated from Enzyme-Treated Zizania latifolia Extract

Abstract

Tricin, a flavone found mainly in rice bran and sugarcane, has various beneficial effects. It has proven to be a clinically safe and selective potent inhibitor of different cancer cell lines. In this study, we evaluated the efficacy of enzyme-treated Zizania latifolia (ETZL) and its major active compound tricin on skin photoaging in SKH-1 hairless mice. Tricin (0.3 mg/kg) and ETZL (50, 150, and 300 mg/kg) were orally administrated to mice for 14 weeks; no cytotoxicity was observed during the entire experimental period. After UVB exposure, we observed significant increases in keratinization, coarse wrinkles, loss of moisture, thickened epidermis, and collagen fiber degradation in the dorsal skin. These features of photoaging were significantly suppressed after oral administration of tricin or ETZL. In addition, the protein expression of collagen effectively increased in ETZL (150 and 300 mg/kg)-treated mice, while the increased metalloproteinase (MMP)-1 and MMP-3 expressions were reduced after exposure to tricin or ETZL, although the effects were not dose-dependent. These data indicate that ETZL may be effective for attenuation of UVB-induced skin damage and photoaging in hairless mice, possibly by inhibiting MMPs expression.

A Natural Flavone Tricin from Grains Can Alleviate Tumor Growth and Lung Metastasis in Colorectal Tumor Mice

“…Recent animal studies demonstrated the broad-spectrum bioactivities of tricin, ranging from anti-obesity effect in high-fatdiet-induced obese mice [ 33 ] to protection against UVB-induced wrinkle formation [ 34 ]. Nevertheless, the anti-metastatic and/or immunomodulatory effects of tricin in colon cancer model have not yet been reported.…”

Enzyme-Treated Zizania latifolia Ethanol Extract Protects from UVA Irradiation-Induced Wrinkle Formation via Inhibition of Lysosome Exocytosis and Reactive Oxygen Species Generation

“…In particular, limited information is available on wrinkle formation through ROS generation and lysosomal exocytosis by UVA irradiation compared with wrinkle formation by UVB irradiation. In addition to the known antioxidant effects of ZLE [ 38 ], it also showed an antiwrinkle effect on UVB-irradiated HDFs and SKH-1 hairless mice by blocking the MAPK signaling pathway resulting in the inhibition of NF-κB and activator protein-1 (AP-1) activities [ 18 , 19 ]. Both ZLE and tricin treatments showed skin protective effects and helped reduce wrinkle formation in UVA-irradiated human dermal fibroblasts and hairless mice in this study.…”

“…The aerial parts of Z. latifolia were purchased from Pureunsan Agricultural Association Corporation (Pocheon, Korea) and ZLE, prepared according to a previously described method [ 18 ], was provided from the BTC Corporation (Sangnok-gu, Ansan, Korea). The concentration of tricin in ZLE was determined by HPLC using an Agilent Infinity 1260 system (Agilent Technologies, Palo Alto, CA, USA), which was measured in our previous study [ 19 ]. The separation of tricin was conducted at 30 °C using a SUPELCO Discovery ® C18 column (4.6 × 250 mm, 5 μm, Merck KGaA).…”

“…The leaves of Z. latifolia show various beneficial effects on the improvement of metabolic and cardiac diseases and hypertension [ 14 , 15 , 16 , 17 ]. Z. latifolia also has protective effects on UVB induced-wrinkle formation and skin moisture content as well as antioxidant activity through regulation of the mitogen-activated protein kinase (MAPK) signaling pathway [ 18 , 19 ]. The leaves of Z. latifolia contain proteins, minerals, vitamins and many bioactive compounds.…”

“…In particular, the flavonoid tricin found in the leaves of Z. latifolia was shown to improve the cutaneous system by regulating antioxidative and anti-inflammatory signaling pathways [ 20 , 21 ]. The concentration of tricin increased in an ethanol extract of the aerial parts of Z. latifolia after they were treated with a mixture of hydrolytic enzymes (cellulase, hemicellulase and pectinase) [ 19 ]. In this study, we investigated the protective effects of enzyme-treated Z. latifolia extract (ZLE) and tricin on skin aging by UVA radiation through inhibition of lysosomal shedding and increased antioxidant activity.…”