A titanium surface modified with zinc-containing nanowires: Enhancing biocompatibility and antibacterial property in vitro

Shao, Shui-yi; Chen, Jiaxi; Tang, Haiyan; Ming, Pan-pan; Yang, Jie; Zhu, Wenqing; Zhang, Songmei; Qiu, Jing

doi:10.1016/j.apsusc.2020.146107

Cited by 33 publications

(28 citation statements)

References 39 publications

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“…In our previous study, we successfully incorporated a novel acid-etched microstructured titanium surface modified with zinc-containing nanowires (Ti-NW-Zn), which proved that a zinc-modified titanium surface has good corrosion resistance and antibacterial activity. The excellent performance of zinc-modified implants was mostly due to the introduction of zinc ions ( Shao et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of Zinc Ions Released From Ti-NW-Zn Surface on Osteogenesis and Angiogenesis In Vitro and in an In Vivo Zebrafish Model

Zhu

et al. 2022

Front. Bioeng. Biotechnol.

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Zinc-modified titanium materials have been widely applied in oral implants. Among them, our previous studies have also successfully prepared a novel acid-etched microstructured titanium surface modified with zinc-containing nanowires (Ti-NW-Zn) and proved its excellent biocompatibility. It is well known that the functional regulation between angiogenesis and osteogenesis is of great importance for bone remodeling around implants. However, there are few reports concerning the biological safety of zinc ions released from materials and the appropriate concentration of released zinc ions which was more conducive to angiogenesis and bone regeneration. In this study, we investigated the effects of zinc ions released from Ti-NW-Zn surfaces on angiogenesis and osteogenesis using the zebrafish model and revealed the relationship between angiogenesis and osteogenesis via HUVECs and MC3T3-E1s in vitro. We found that the zinc ions released from Ti-NW-Zn surfaces, with a concentration lower than median lethal concentrations (LCs) of zebrafish, were biologically safe and promote osteogenesis and angiogenesis in vivo. Moreover, the proper concentration of zinc ions could induce the proliferation of HUVECs and osteogenic differentiation. The positive effects of the appropriate concentration of zinc ions on osteoblast behaviors might be regulated by activating the MAPK/ERK signaling pathway. These aspects may provide new sights into the mechanisms underlying zinc-modified titanium surfaces between osteogenesis and angiogenesis, to lay the foundation for further improving the materials, meanwhile, promoting the applications in dentistry.

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Section: Discussionmentioning

confidence: 99%

Effects of Zinc Ions Released From Ti-NW-Zn Surface on Osteogenesis and Angiogenesis In Vitro and in an In Vivo Zebrafish Model

Zhu

et al. 2022

Front. Bioeng. Biotechnol.

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“…In addition, our previous studies have also found that zinc-modi ed titanium surface has good corrosion resistance and antibacterial ability. The excellent performance of zinc-modi ed implants mainly bene ts from the introduction of zinc ions [25]. Zebra sh share 70 percent of their genetic homology with humans [26].…”

Section: Discussionmentioning

confidence: 99%

Effects of Zinc Ions Released from Ti-NW-Zn Surface on Osteogenesis and Angiogenesis in Vitro and in an in Vivo Zebrafish Model

Zhu

et al. 2021

Preprint

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Objective: Zinc-modified titanium materials have been widely applied in oral implants, among them, our previous studies have also successfully prepared a novel acid-etched micro-structured titanium surface modified with zinc-containing nanowires (Ti-NW-Zn). However, there are very few reports concerned about the biological safeties of zinc ions released from material surface and the appropriate concentration range of released zinc ions which was more conducive to angiogenesis and bone regeneration. Danio rerio (Zebrafish) represents a powerful alternative in vivo model to study both angiogenesis and osteogenesis. Then, the aim of this study is to investigate the effects of zinc ions released from Ti-NW-Zn surfaces on angiogenesis and osteogenesis via zebrafish model, and further revealed the relationship between angiogenesis and osteogenesis via HUVECs and MC3T3-E1s in vitro models.Materials and methods: Tg (Fli‐1:EGFP)y1 zebrafish embryos were used to investigate the in vivo angiogenesis exposure to zinc ions, Wild-type Zebrafish (Danio rerio) adult fish and their embryos were used to investigate the in vivo bone regeneration and survival rate. Cell experiments including proliferation, adhesion, osteogenic differentiation and signaling pathway experiments were carried out by CCK-8, Laser scanning confocal microscope and Western blot, etc. Zinc ion release assay was detected by a Zinc assay kit.Results: The zinc ions released from Ti-NW-Zn surfaces were far lower than median lethal concentrations (LCs) of both embryos and adult fish, which was beneficial to osteogenesis and angiogenesis promotion of zebrafish model. Moreover, the appropriate concentration range of zinc ions was 1-2 ppm in vitro models, which could induce HUVECs proliferations, and the conditioned medium (CM) collected from the supernatant of 1-2 ppm zinc ions-induced HUVECs medium could obviously promote MC3T3-E1 osteoblasts behaviors via activated the MAPK/ERK signaling pathway.Conclusion: Zinc ions released from Ti-NW-Zn surfaces was at a biological safe and appropriate concentration, which could promote the angiogenesis and osteogenesis in vivo and vitro. The positive effects of the appropriate concentration of zinc ions on osteoblast behaviors might be regulated by activating the MAPK/ERK signaling pathway.

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“… 106 Also, Shao et al detected improved biocompatibility and antibacterial properties of zinc-containing nanowires. 107 Further studies are required to demonstrate the molecular mechanism of zinc ions in bone metabolism.…”

Section: Peri-implant Bone Remodeling Of Implants With Metal Ions Inc...mentioning

confidence: 99%

Impact of exogenous metal ions on peri-implant bone metabolism: a review

2021

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A titanium surface modified with zinc-containing nanowires: Enhancing biocompatibility and antibacterial property in vitro

Cited by 33 publications

References 39 publications

Effects of Zinc Ions Released From Ti-NW-Zn Surface on Osteogenesis and Angiogenesis In Vitro and in an In Vivo Zebrafish Model

Effects of Zinc Ions Released From Ti-NW-Zn Surface on Osteogenesis and Angiogenesis In Vitro and in an In Vivo Zebrafish Model

Effects of Zinc Ions Released from Ti-NW-Zn Surface on Osteogenesis and Angiogenesis in Vitro and in an in Vivo Zebrafish Model

Impact of exogenous metal ions on peri-implant bone metabolism: a review

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